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FRP792_insul-(lexA-box)2-PminCYC1-Citrine-TCYC1

Plasmid

#58433

Purpose

Induces Citrine expression from a promoter containing 2 lexA boxes in yeast cells

Depositor

Joerg Stelling

Publication

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

CitrineA206K

Expression

Yeast

Promoter

insul-(lexA-box)2-PminCYC1

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PhyB-mCherry-PEX3

Plasmid

#51572

Purpose

Constitutively express peroxisome localized PhyB in Saccharomyces cerevisiae

Depositor

Chao Tang

Publication

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PhyB (PHYB Mustard Weed)

Tags

PEX3 and mCherry

Expression

Yeast

Mutation

1-908aa only

Promoter

ADH1pr

Available since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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PhyB-mCherry-Htb2

Plasmid

#51569

Purpose

Constitutively express nuclear localized PhyB in Saccharomyces cerevisiae

Depositor

Chao Tang

Publication

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PhyB (PHYB Mustard Weed)

Tags

HTB2 and mCherry

Expression

Yeast

Mutation

1-908aa only

Promoter

ADH1pr

Available since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only

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M3925 trp1::KanMX3 Disruptor Converter

Plasmid

#51681

Purpose

Yeast marker swap plasmid for converting Trp1 to KanMX3

Depositor

David Stillman

Publication

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

KanMX3

Use

Yeast marker swap

Expression

Yeast

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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M3929 his3::KanMX3 Disruptor Converter

Plasmid

#51679

Purpose

Yeast marker swap plasmid for converting HIS3 to KanMX3

Depositor

David Stillman

Publication

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

KanMX3

Use

Yeast marker swap

Expression

Yeast

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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HO-pMET-poly-KanMX4-HO

Plasmid

#51665

Purpose

yeast plasmid for integration of target sequence at HO locus, Kan selection, pMET inducible promoter

Depositor

David Stillman

Publication

Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

Type

Empty backbone

Use

Yeast integration

Expression

Yeast

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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pICXNH3

Plasmid

#49020

Purpose

FX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag and 3C protease cleavage site

Depositor

Raimund Dutzler, Eric Geertsma

Publication

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Yeast

Promoter

AOX1

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pAAH5

Plasmid

#41829

Purpose

Used as a PCR template for tagging yeast proteins with the SNAP tag by homologous recombination and selection for hygromycin resistance.

Depositor

Melissa Moore

Publication

Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

Type

Empty backbone

Use

Pcr template

Expression

Bacterial and Yeast

Promoter

T7,TEF

Available since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoPA-TagRFP-SpHis5

Plasmid

#44854

Purpose

Yeast tagging vector to create gene fusion with yeast optimized photoactivatable TagRFP with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoPA-TagRFP

Expression

Yeast

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pETCON-HtsptLB12v.3

Plasmid

#45121

Depositor

David Baker

Publication

Procko et al J Mol Biol. 2013 Sep 23;425(18):3563-75.

Insert

HtsptLB12v.3

Tags

Aga2p for yeast surface display and c-myc epitope…

Expression

Yeast

Promoter

GAL

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pFA6a-link-yomKeima-Kan

Plasmid

#44902

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mKeima with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomKeima

Expression

Yeast

Available since

May 17, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoClover-SpHis5

Plasmid

#44857

Purpose

Yeast tagging vector to create gene fusion with yeast optimized Clover with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoClover

Expression

Yeast

Available since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

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pAG306-GPD-VMA1 chr I

Plasmid

#41896

Purpose

Yeast expression of VMA1.

Depositor

Dan Gottschling

Publication

Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

Insert

VMA1 (VMA1 Budding Yeast)

Expression

Yeast

Mutation

Contains silent mutation at 342A-G and L87S mutat…

Promoter

GPD

Available since

April 17, 2013

Availability

Academic Institutions and Nonprofits only

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pCT-4M5.3

Plasmid

#41844

Depositor

Dane Wittrup

Publication

Midelfort et al J Mol Biol. 2004 Oct 22;343(3):685-701.

Insert

4M5.3

Tags

HA and c-myc

Expression

Yeast

Available since

Jan. 29, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTAL5-BB

Plasmid

#36033

Depositor

Tom Ellis

Publication

Blount et al PLoS One. 2012;7(3):e33279. Epub 2012 Ma

Type

Empty backbone

Expression

Yeast

Promoter

PGAL1

Available since

July 3, 2012

Availability

Academic Institutions and Nonprofits only

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pDRf1

Plasmid

#36027

Depositor

Wolf Frommer, Dominique Loque

Publication

Loque et al Nature. 2007 Mar 8;446(7132):195-8. Epub

Type

Empty backbone

Expression

Yeast

Available since

May 30, 2012

Availability

Academic Institutions and Nonprofits only

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pDS271

Plasmid

#34974

Depositor

Michael Glotzer

Publication

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2-GFP-LOVpep (MID2 A. sativa, Budding Yeast)

Expression

Yeast

Mutation

T406A, T407A, V416I

Promoter

TEF

Available since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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413-Met25 Kar2 TorsinA GFP

Plasmid

#32119

Depositor

Susan Lindquist

Publication

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

TorsinA (TOR1A Human)

Tags

GFP and Kar2

Expression

Yeast

Available since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
416Gal-FUS-R521C-YFP

Plasmid

#29622

Depositor

Aaron Gitler

Publication

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Tags

YFP

Expression

Yeast

Mutation

R521C

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pDR-GW FLIPglu-600μΔ13V

Plasmid

#28001

Depositor

Wolf Frommer

Publication

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

FLIPglu-600μΔ13V

Tags

eCFP and eYFP

Expression

Yeast

Mutation

F16A in mglB

Available since

March 31, 2011

Availability

Academic Institutions and Nonprofits only