8,436 results
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Plasmid#226181PurposeExpresses Cas9 and gRNA targeting KU70 gene in K. phaffii.DepositorInserttRNA-sgRNA-tRNA
UseCRISPRExpressionBacterial and YeastPromoterTEF1p (Komagataella phaffii)Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYAL_HLA-A2_MART1
Plasmid#171177PurposeDisplays MART-1 peptide in HLA-A*02 as a single-chain trimerDepositorInsertMART1_HLA-A2*01 single-chain trimer fusion
ExpressionBacterial and YeastPromoterGAL1Available sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCdOpt-BMX
Plasmid#203929PurposeThis plasmid contains a codon optimized BleMX drug resistance cassette for generating gene deletions in C. glabrata, C. auris, C. albicans or S. cerevisiaeDepositorInsertBleMX
ExpressionYeastMutationBleMX was codon optimized for Candida albicans or…Available sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pABY-c45
Plasmid#201771PurposeExpresses mNeonGreen-tagged NbALFA under TEF1 promoter in yeastDepositorInsertNbALFA
Tags40aa Linker, mNeonGreenExpressionYeastPromoterTEF1Available sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSC5GGv1
Plasmid#194645PurposepSC5 Golden-Gate Cloning Plasmid (BsaI - Site 1)DepositorTypeEmpty backboneUseSynthetic Biology; Diatom expressionExpressionBacterial and YeastAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS306-OsTIR1F74A
Plasmid#198416PurposeExpresses OsTIR1F74A under the control of ADH1 promoter in budding yeastDepositorInsertOsTIR1F74A
ExpressionYeastPromoterADH1 promoterAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSC5
Plasmid#188602PurposeSuperior Conjugative Plasmid to Diverse YeastDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-ATG8(416)/GFP-AUT7(416)
Plasmid#160389PurposeExpresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter , NAT selectionDepositorInsertautophagy-related 8 (ATG8 Budding Yeast)
UseCen/arsExpressionYeastPromoterEndogenous ATG8 promoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNH605-PACE-YFP
Plasmid#172002PurposeFluorescent reporter for Rpn4 activityDepositorInsert4xPACE-YFP
UseVector for genomic integration into the yeast gen…ExpressionYeastPromoterCYC1 core promotor with 4 tandem repeats of Rpn4 …Available sinceNov. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pYM28-BirA*
Plasmid#160290PurposeUsed for C-terminal BirA* tagging of proteins in Saccharomyces cerevisiae; Selection marker: HIS3DepositorInsertBirA*
ExpressionYeastAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGBW-m4046483
Plasmid#153868PurposeYeast expression plasmid for SARS-CoV-2 nsp3DepositorInsertSARS-CoV-2 nsp3 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
TagsCleavable thrombin;6xHISExpressionYeastMutationwtPromoterpGAL1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBKWH-p53
Plasmid#133900PurposePositive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWHDepositorAvailable sinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM10
Plasmid#133639PurposeIntegration of VPS4-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast late endosome.DepositorAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM18
Plasmid#133631PurposeIntegration of NAB2-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast nucleus.DepositorAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
BB3cN_pGAP_23*_pPFK300_Cas9
Plasmid#104911PurposehCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTCDepositorInsertsHH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
UseCRISPRExpressionYeastAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCfB8644
Plasmid#126919PurposePlasmid containing integration cassette at IntC2 integration site in Yarrowia lipolytica for overexpression of XdCrtI and XdCrtYBDepositorInsertXdCrtI and HMG1
ExpressionYeastAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-N2H-N2
Plasmid#125548PurposeGateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment2DepositorTypeEmpty backboneUseIn vitro expressionTagsNanoLuc fragment2 attached to n-terminus of Gatew…ExpressionMammalian and YeastPromotersynthetic promoter containing yeast, mammalian, a…Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FRP2374
Plasmid#127579PurposeRepressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Met15 selection markerDepositorInsertPRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
UseSynthetic Biology; Shuttle vectorExpressionYeastAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pICE ADE2
Plasmid#122876PurposeADE2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailable sinceMay 25, 2019AvailabilityAcademic Institutions and Nonprofits only