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8,265 results

Showing: 81 - 100 of 8265 results

  8. pRS416-yZ3EV-Z3pr-yEGFP (RB3579)

    Plasmid
    #69100
    Purpose
    URA+ yeast shuttle vector containing yZ3EV TF cassette and a Z3pr driving expression of yEGFP
    Depositor
    David Botstein
    Inserts
    yZ3EV cassette
    Z3 promoter
    yEGFP
    Expression
    Yeast
    Available Since
    Sept. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    69100-plasmid-map-sequence-id-122356

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  10. pCfB2188-PrARG8-GFP::URA3

    Plasmid
    #216512
    Purpose
    Integrative fluorescent reporter for amino acid biosynthesis sensing pathways, comprising GFP driven by the ARG8 promoter within the Easy clone vector, requires NotI digestion for integration in yeast
    Depositor
    Juha Saarikangas
    Article
    Paukstyte et al Mol Biol Cell. 2025 Apr 2:mbcE24040162.
    Insert
    PrARG8-yeGFP
    Use
    Cre/Lox; Integrative
    Expression
    Yeast
    Promoter
    ARG8
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    216512-plasmid-map-sequence-id-429384

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  11. pCfB2197-PrGNP1-mK2-NatMX

    Plasmid
    #216513
    Purpose
    Integrative fluorescent reporter for amino acid uptake sensing pathway, comprising mKate2 driven by the GNP1 promoter within the Easy clone vector, requires NotI digestion for integration in yeast
    Depositor
    Juha Saarikangas
    Article
    Paukstyte et al Mol Biol Cell. 2025 Apr 2:mbcE24040162.
    Insert
    PrGnp1-mKate2
    Use
    Cre/Lox; Integrative
    Expression
    Yeast
    Promoter
    GNP1
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    216513-plasmid-map-sequence-id-429383

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  17. TDH3pro-rtTA-tetO7pro-T7pol-pRS413

    Plasmid
    #239293
    Purpose
    Expresses the reverse tetracycline activator (rtTA) and T7 polymerase; Plasmid #1 of the tet-on overexpression system
    Depositor
    Anita Hopper
    Article
    Nostramo et al Mol Cell. 2025 Feb 20;85(4):726-741.e6.
    Inserts
    T7 polymerase with NLS and P278L
    reverse tetracycline transactivator (rtTA)
    Expression
    Yeast
    Mutation
    Contains M2-SE-G72P mutations, described in Addge…
    Promoter
    TDH3 and tetracycline-responsive element (TRE) an…
    Available Since
    June 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    239293-plasmid-map-sequence-id-476828

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  20. pCAS

    Plasmid
    #60847
    Purpose
    Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast
    Depositor
    Jamie Cate
    Article
    Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife
    Inserts
    S. pyogenese Cas9
    RNA pol III promoter (tRNA-Tyr)
    hepatitis delta virus ribozyme, genomic
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    NLS/His8 and TTT 3' extension prior to sgRNA
    Expression
    Bacterial and Yeast
    Mutation
    L4 is UUCG tetraloop and guide targets LYP1 (CATA…
    Available Since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    60847-plasmid-map-sequence-id-197511

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Showing: 81 - 100 of 8265 results