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Plasmid#166140PurposeEGFP expression under the control of the Galactose-inducible promoter yeast. Contains CEN/ARS element for low copy within yeast.DepositorArticleInsertEGFP
UseTagsExpressionYeastMutationPromoterGAL1Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBHM2404
Plasmid#173441PurposepRS316-mNeonGreen-CBP-2XFLAG-NAT1, contains C. neoformans optimized mNeonGreen and NAT1 marker for use as PCR templateDepositorInsertmNeonGreen-CBP-2XFLAG-NAT1
UseTagsCBP-2xFLAGExpressionBacterial and YeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBHM2406
Plasmid#173442PurposepRS316-mNeonGreen-CBP-2XFLAG-HYGB, contains C. neoformans optimized mNeonGreen and HYG marker for use as PCR templateDepositorInsertmNeonGreen-CBP-2XFLAG-HYGB
UseTagsCBP-2xFLAGExpressionBacterial and YeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
TurboID-V5_pRS415
Plasmid#107167Purposeexpresses V5-tagged TurboID in the yeast cytosolDepositorInsertTurboID (BirA mutant)
UseTagsExpressionYeastMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pS1643 (pFA6-NATMX-CDC42pr-yGFP1-10)
Plasmid#86420PurposeN-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoterDepositorInsertCDC42pr-GFP1-10
UseTagsGFP1-10ExpressionYeastMutationPromoterAvailable sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAS9cd
Plasmid#141252PurposeConstitutive expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterTEF1 (Cas9), pSNR52 (gRNA)Available sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-Strep-tagII-HIS3MX6
Plasmid#20756DepositorTypeEmpty backboneUsePcr-based c-terminal taggingTagsExpressionYeastMutationPromoterAvailable sinceMarch 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTA-HUH
Plasmid#221152PurposeHisG-URA3-HisG recycling type marker in pTAs for Yarrowia lipolyticaDepositorInsertHisG-URA3-HisG
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
miniTurbo-V5_pRS415
Plasmid#107168Purposeexpresses V5-tagged miniTurbo in the yeast cytosolDepositorInsertminiTurbo (BirA mutant)
UseTagsExpressionYeastMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
JME4580
Plasmid#129659PurposeHPHex_CrisprCas9-yl_RFP: CAS9 vector with Hygromycinex marker for gRNA cloning using GoldenGateDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
JME4472
Plasmid#129658PurposeURA3ex_CrisprCas9-yl_RFP: CAS9 vector with URA3ex marker for gRNA cloning using GoldenGateDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
POM43-mTFP1
Plasmid#69853PurposeN-terminal tagging with mTFP1 using LEU2 as a selection marker in S. cerevisiae.DepositorInsertmTFP1 minus start and stop codon
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMetYC-Dest
Plasmid#105081PurposeYeast mating-based Split Ubiquitin Assay, bait vector for Gateway cloning. Cub-PLV does not contain a start codon.DepositorTypeEmpty backboneUseTagsCub-PLVExpressionYeastMutationPromoterAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
p406ADH1
Plasmid#15974DepositorInsertADH1 promoter + polylinker + CYC1 terminator (ADH1 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationADH1 promoter is between SacI and XbaI restrictio…PromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pNX32-Dest
Plasmid#105083PurposeYeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloningDepositorTypeEmpty backboneUseTagsNubGExpressionYeastMutationPromoterAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
SJZ9-amdS
Plasmid#228261PurposeTemplate plasmid for yeast gene knockout, allows the use of acetamide as sole nitrogen sourceDepositorInsertamdS
UseTagsExpressionYeastMutationPromoterENO2 S.c.Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
p404TEF1
Plasmid#15972DepositorInsertTEF1 promoter + polylinker + CYC1 terminator (TEF1 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationTEF1 promoter is between SacI and XbaI restrictio…PromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pXN22-Dest
Plasmid#105085PurposeYeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloningDepositorTypeEmpty backboneUseTagsNubG-3xHAExpressionYeastMutationPromoterAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymScarletI3-URA3
Plasmid#224075PurposeYeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with URA3 selectionDepositorInsertymScarletI3
UseTagsymScarletI3ExpressionYeastMutationPromoterAvailable sinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymScarletI3-Kan
Plasmid#224077PurposeYeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with Kan selectionDepositorInsertymScarletI3
UseTagsymScarletI3ExpressionYeastMutationPromoterAvailable sinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only