8,135 results

1. pRS306-URA3-PHIS3-AqLumSynth –Sapphire

   Plasmid

   #116931

   Purpose

   Yeast integrative vector for the exression of the 20nm GEM under the HIS3 promoter

   Depositor

   Liam Holt

   Article

   Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

   Insert

   lumazine synthase
   

   Tags

   Sapphire

   Expression

   Yeast

   Promoter

   HIS3

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBHM2673

   Plasmid

   #241720

   Purpose

   Integration of DNA into Cryptococcus neoformans Safe Haven 1 locus, contains BSD marker for selection on blasticidin S

   Depositor

   Hiten Madhani

   Article

   Boucher et al Microbiol Spectr. 2025 Sep 25:e0193525.

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Sept. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYES2-HTH-HHT2

   Plasmid

   #86482

   Purpose

   yeast expression of HHT2 with His10-TEV-HA tag

   Depositor

   Akash Gunjan

   Article

   Singh et al Nat Cell Biol. 2009 Aug;11(8):925-33. do

   Insert

   HHT2 (HHT2 Budding Yeast)
   

   Tags

   HIS10-TEV-HA (HTH)

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   Jan. 27, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. p415 TEF roGFP2‐Tsa2ΔCR

   Plasmid

   #83238

   Purpose

   The genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.

   Depositor

   Tobias Dick

   Article

   Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do

   Insert

   roGFP2-Tsa2dCr
   

   Tags

   roGFP2 fusion with Tsa2dCr

   Expression

   Yeast

   Promoter

   TEF

   Available Since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGKB

   Plasmid

   #85077

   Purpose

   expression in Pichia pastoris

   Depositor

   Sheng Yang

   Article

   Yang et al Yeast. 2014 Apr;31(4):115-25.

   Type

   Empty backbone

   Expression

   Yeast

   Promoter

   GAP

   Available Since

   March 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSP323 pRS306 pRPS2-Mkk2DS-NLS-mCherry

   Plasmid

   #68280

   Purpose

   SKARS: Mpk1 MAPK activity sensor: Mkk2DS (1-100) 2xNLS mCherry

   Depositor

   Serge Pelet

   Article

   Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

   Insert

   Mkk2DS NLS mCherry (MKK2 Budding Yeast)
   

   Tags

   mCherry

   Expression

   Yeast

   Promoter

   RPS2

   Available Since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUG34-NES-EGFP-cPHx3

   Plasmid

   #183669

   Purpose

   PtdIns(3,4)P2 biosensor for expression in yeast

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   PLEKHA1 (PLEKHA1 Frog, Human)
   

   Tags

   X. laevis map2k1.L(32-44)-EGFP

   Expression

   Yeast

   Mutation

   Amino acids 169-329 (tandem trimer)

   Promoter

   MET25

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFA6a-link-yoEGFP-CaURA3

   Plasmid

   #44872

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized EGFP with CaUra3 selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoEGFP
   

   Expression

   Yeast

   Available Since

   May 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAP01

   Plasmid

   #59981

   Purpose

   The function of this material is to facilitate the capture and expression of bacterial gene clusters.

   Depositor

   Bradley Moore

   Article

   Yamanaka et al Proc Natl Acad Sci U S A. 2014 Feb 4;111

   Type

   Empty backbone

   Use

   Synthetic Biology; Transformation-associated reco…

   Tags

   no

   Expression

   Bacterial and Yeast

   Promoter

   no

   Available Since

   Nov. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-ATG8(416)/GFP-AUT7(416)

    Plasmid

    #49425

    Purpose

    Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS416 vector.

    Depositor

    Daniel Klionsky

    Article

    Guan et al Mol Biol Cell. 2001 Dec;12(12):3821-38.

    Insert

    autophagy-related 8 (ATG8 Budding Yeast)
    

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Promoter

    ATG8

    Available Since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFA6a-TID-HA3-KanMX6

    Plasmid

    #227980

    Purpose

    To insert biotin ligase, TurboID, at the C-terminus of genes; yeast BioID, KanMX6 marker

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    TurboID
    

    Tags

    HA tag

    Expression

    Yeast

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCast

    Plasmid

    #200169

    Purpose

    E. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozyme

    Depositor

    Yong-Su Jin

    Article

    Kuanyshev et al Biotechnol Bioeng. 2021 Jan;118(1):372-3

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDP0437

    Plasmid

    #221127

    Purpose

    Plasmid for generating integration cassette to C-terminally tag gene of interest with pHluorin::HPHMX

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    pHluorin
    

    Expression

    Yeast

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEasyG1_nat

    Plasmid

    #184911

    Purpose

    Template to generate via PCR a single gRNA for expression in S. cerevisiae

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Expression

    Bacterial and Yeast

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRS416-HIS3p-TID-FKBP-HA3

    Plasmid

    #228006

    Purpose

    Expresses TurboID-FKBP fusion from HIS3 promoter; yeast BioID

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    TurboID-FKBP
    

    Tags

    HA tag

    Expression

    Yeast

    Promoter

    HIS3

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFa6-TID-FKBP-3HA-KanMX6

    Plasmid

    #228024

    Purpose

    To integrate TID-FKBP fusion at yeast promoter; yeast BioID with KanMX6 marker

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    TurboID-FKBP
    

    Tags

    HA tag

    Expression

    Yeast

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. TurboID-V5_pRS415

    Plasmid

    #107167

    Purpose

    expresses V5-tagged TurboID in the yeast cytosol

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Expression

    Yeast

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMetYCgate

    Plasmid

    #105074

    Purpose

    Yeast mating-based Split Ubiquitin Assay, bait vector for in vivo cloning

    Depositor

    Wolf Frommer

    Article

    Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

    Type

    Empty backbone

    Tags

    Cub-PLV

    Expression

    Yeast

    Available Since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYES2-gRNA-hyg-MCS

    Plasmid

    #107734

    Purpose

    E. coli-S. cerevisiae shuttle plasmid harbors gRNA expression cassettes targeting S. cerevisiae chromosomal X-3 site (HXK1 gene). Can function as a gRNA expression empty backbone plasmid.

    Depositor

    Sheng Yang

    Article

    Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

    Insert

    X-3 site gRNA expression cassettes
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    SNR52p

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNXgate33-3HA

    Plasmid

    #105075

    Purpose

    Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

    Depositor

    Wolf Frommer

    Article

    Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

    Type

    Empty backbone

    Tags

    3xHA and NubG

    Expression

    Yeast

    Available Since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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