8,400 results
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Plasmid#108687PurposePlasmid for assembly of donor DNA templates consisting of three expression unitsDepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only
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pUDP013
Plasmid#103873PurposeBroad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Ogataea (para)polymorphaDepositorInsertHH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha
UseCRISPRExpressionYeastPromoterScTDH3Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMEL10
Plasmid#107916PurposeURA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y
UseCRISPRExpressionYeastAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXNubWtgate
Plasmid#105080PurposeYeast mating-based Split Ubiquitin Assay, positive controlDepositorTypeEmpty backboneTagsNubIExpressionYeastAvailable SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMetOYC-Dest
Plasmid#105082PurposeYeast mating-based Split Ubiquitin Assay, bait vector for Gateway cloningDepositorTypeEmpty backboneTagsCub-PLV and mOST4ExpressionYeastAvailable SinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAV103
Plasmid#63193PurposepRS403 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionWorm and YeastAvailable SinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPMVAu5 (R)
Plasmid#98296PurposeEnhancing the upper-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.DepositorInsertPSSA1-ERG10-TNAT1-PRPL4A-ERG13-TEFM1-PRPL15A-tHMG1-TEBS1
ExpressionYeastAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB4279
Plasmid#98698PurposeA control plasmid containing the intact bsdMX markerDepositorInsertCas9
UseCRISPRExpressionYeastAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCTcon2-5INS_16
Plasmid#102292PurposeYeast Surface Display of 5Ins_16 scFVDepositorInsertInsulin binding scFV
Tags3x(G4S) Linker, G3S Linker, HA, and MycExpressionYeastPromoterGAL1Available SinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDR FLIP43 GPS1
Plasmid#101084PurposeGA biosensor expression plasmidDepositorInsertGPS1
Tags6HIS and cMycExpressionYeastPromoterpPMA1Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS423-CUP1-His-Ubi
Plasmid#99541PurposeYeast episomal vector for expression of His-tagged ubiquitin; HIS3 markerDepositorAvailable SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
YEp195-CUP1-His-Smt3
Plasmid#99538PurposeYeast episomal vector for expression of His-tagged Smt3 (SUMO); URA3 markerDepositorAvailable SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-CLIP-KanMX6
Plasmid#87025PurposeTag S. pombe genes in C terminal with CLIPDepositorInsertCLIP
ExpressionBacterial and YeastAvailable SinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mEGFP-KanMX6
Plasmid#87023PurposeTag S. pombe genes in C terminal with mEGFPDepositorInsertmEGFP
ExpressionBacterial and YeastAvailable SinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
E2-Crimson-FKBP(M)
Plasmid#85002PurposeExpresses E2-Crimson-FKBP(M)DepositorInsertE2-Crimson-FKBP(M)
ExpressionYeastAvailable SinceDec. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB3051(gRNA X-3 XI-2 XII-2)
Plasmid#73293PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-3, XI-2, and XII-2DepositorInsertguiding RNA
UseCRISPR; GrnaExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-hygMV
Plasmid#73891Purposefission yeast drug selection markerDepositorInserthygMV
ExpressionBacterial and YeastPromoterpombe TEFAvailable SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pILGFP92
Plasmid#83549PurposeUsed to evaluate the expression output of TDH3 promoter with yEGFP used as the reporter gene.DepositorInsertTDH3 promoter-yEGFP
ExpressionYeastPromoterTDH3Available SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS35
Plasmid#83797PurposeUseful for tagging yeast proteins with C-terminal mCherry tag; hygromycin-B selectionDepositorInsertmCherry
TagsmCherryExpressionYeastAvailable SinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHLU
Plasmid#64167Purposeharbours marker genes HIS3, LEU2 and URA3DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only