8,265 results

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PCUP1-DuDre-Atg8-404

Plasmid

#69201

Purpose

RFP-Atg8 (PAS marker) with improved property

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

DuDre-Atg8

Tags

DsRed Express 2

Expression

Yeast

Available Since

Sept. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pAG305GAL-ccdB

Plasmid

#14137

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Expression

Yeast

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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pMEG_YLCas9_empty

Plasmid

#117826

Purpose

CRISPR/Cas9 plasmid for GoldenMOCS in Y. lipolytica

Depositor

Michael Sauer

Article

Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

Insert

YlpTEF_hCas9_YlCyc1TT

Expression

Bacterial and Yeast

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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FRP792_insul-(lexA-box)2-PminCYC1-Citrine-TCYC1

Plasmid

#58433

Purpose

Induces Citrine expression from a promoter containing 2 lexA boxes in yeast cells

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

CitrineA206K

Expression

Yeast

Promoter

insul-(lexA-box)2-PminCYC1

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pRS426-GFP-COF1

Plasmid

#31844

Depositor

David Drubin

Article

Okreglak et al J Cell Biol. 2007 Sep 24;178(7):1251-64.

Insert

Cofilin (COF1 Budding Yeast)

Tags

GFP (S65T)

Expression

Yeast

Promoter

T7

Available Since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only
p413_Csy4NLS

Plasmid

#103139

Purpose

Expresses Csy4-NLS under TEF promoter

Depositor

Jens Nielsen

Article

Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

Insert

Csy4

Tags

NLS

Expression

Yeast

Promoter

TEF

Available Since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pBKWH-p53

Plasmid

#133900

Purpose

Positive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWH

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Insert

p53 (Trp53 Mouse)

Expression

Yeast

Promoter

T7

Available Since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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pFA6a-mNeonGreen-HIS3MX6

Plasmid

#129100

Purpose

For tagging genes at 3'end (C-terminal end of protein) in S. cerevisiae with mNeonGreen

Depositor

Wei-Lih Lee

Insert

mNeonGreen

Expression

Yeast

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pDRFLIP38-AtLEA4-5

Plasmid

#178189

Purpose

SED1 biosensor for expression in Saccharomyces cerevisiae

Depositor

Cesar Cuevas-Velazquez, Jose Dinneny

Article

Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

Insert

SED1 biosensor

Expression

Yeast

Promoter

pPMA1

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAG416GPD-Cerulean-VPS21

Plasmid

#18842

Depositor

Susan Lindquist

Article

Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

VPS21 (VPS21 Budding Yeast)

Tags

Cerulean

Expression

Yeast

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
ZJOM30

Plasmid

#133673

Purpose

Integration of ERG6-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Present on ER and lipid droplets.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

p1k-ERG6-GFP (ERG6 Budding Yeast)

Tags

EGFP

Expression

Yeast

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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ZJOM20

Plasmid

#133644

Purpose

Integration of PEX1-2GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast peroxisome.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

p1k-PEX1-2GFP (PEX1 Budding Yeast)

Tags

EGFP

Expression

Yeast

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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EC2_3_dCas9_Mxi1_sgRNA

Plasmid

#163708

Purpose

Yeast low copy plasmid with dCas9, sgRNA expression cassette and Mxi1 transcriptional repressor

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Inserts

dCas9
Mxi1+SV40 NLS

Expression

Yeast

Mutation

Point mutations D10A and H840A

Promoter

ScTEF1

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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HsSPHK1

Plasmid

#118091

Purpose

Expresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)

Depositor

Kevin Lynch

Article

Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do

Insert

Homo sapiens Sphingosine Kinase 1

Tags

FLAG

Expression

Yeast

Promoter

GAL1, 10

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221-Sen1

Plasmid

#99994

Purpose

Shuttle yeast Sen1 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Sen1 (SEN1 Budding Yeast)

Expression

Yeast

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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p416 25Q GAL

Plasmid

#1185

Depositor

Susan Lindquist

Article

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 25Q (HTT Human)

Tags

GFP

Expression

Yeast

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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pAG416GPD-Cerulean-YPT31

Plasmid

#18849

Depositor

Susan Lindquist

Article

Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

YPT31 (YPT31 Budding Yeast)

Tags

Cerulean

Expression

Yeast

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pBS-SKII-3XHA-HALO-URA

Plasmid

#188932

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-HALO-URA

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAG415GPD-EGFP-ccdB

Plasmid

#14314

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Yeast

Available Since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoSuperfolderGFP-CaURA3

Plasmid

#44873

Purpose

Yeast tagging vector to create gene fusion with yeast optimized SuperfolderGFP with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoSuperfolderGFP

Expression

Yeast

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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