8,057 results

1. pILGFP5EC4

   Plasmid

   #185880

   Purpose

   A plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (9 copy)

   Depositor

   Claudia Vickers

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pILGFP3AG4ARSd

   Plasmid

   #185876

   Purpose

   A plasmid without an ARS sequence for in vivo gene amplification (HamAmp) at RPL25 locus (~12 copy)

   Depositor

   Claudia Vickers

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDS257

   Plasmid

   #34971

   Purpose

   Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep.

   Depositor

   Michael Glotzer

   Article

   Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

   Insert

   Mid2(SS/TM)-GFP-LOVpep
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS406-PHO5-GFP-hFAPP1 PH domain

   Plasmid

   #58723

   Purpose

   Expresses GFP-Tagged human FAPP1 PH domain in yeast

   Depositor

   Tim Levine

   Article

   Levine et al Curr Biol. 2002 Apr 30;12(9):695-704.

   Insert

   Four-phosphate-adaptor protein 1 PH domain (PLEKHA3 Human)
   

   Use

   Tags

   GFP and Myc

   Expression

   Yeast

   Mutation

   Promoter

   PHO5

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pRS305-LEU2-pHIS3-SV40NLS-PfV-GS-GFPMUT3

   Plasmid

   #203657

   Purpose

   Yeast integrative vector for the expression of the GFPMUT3-tagged nuclear localizing 40nm nucGEMs under the HIS3 promoter.

   Depositor

   Liam Holt

   Article

   Shu T et et al bioRxiv 2022

   Insert

   PfV
   

   Use

   Tags

   GFPMUT3

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYSD300

   Plasmid

   #212930

   Purpose

   Encodes the S. cerevisiae Aga1 protein (Part3) with pGBK1 promoter, tENO2 terminator, HIS3 selectable marker and CEN/ARS yeast origin.

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   AGA1 (AGA1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. PHBA02

   Plasmid

   #83536

   Purpose

   Constitutive TEF1 promoter mediated expression of the UBiC and ARO4* genes

   Depositor

   Claudia Vickers

   Article

   Williams et al Metab Eng Commun. 2016 May 11;3:142-152.

   Insert

   pTEF1-ARO4*-CYC1t-pTEF1-UBiC-CYC1t
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pTEF1

   Available since

   Dec. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. 3413 pBF339 Bcl-XL

   Plasmid

   #8773

   Depositor

   Stanley Korsmeyer

   Article

   Gross et al Mol Cell Biol 2000 May;20(9):3125-36.

   Insert

   Bcl-XL (Bcl2l1 Mouse)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

9. pXP318

   Plasmid

   #26837

   Depositor

   Nancy DaSilva, Suzanne Sandmeyer

   Article

   Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 3, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMJ123: pRS426-His-MBP-SpuFz1_PSP1

    Plasmid

    #205256

    Purpose

    S. cerevisiae expression vector for SpuFz1 reprogrammed guide (targeting PSP1)

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1
    

    Use

    Tags

    10xHis, MBP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. RIB VP64 R1

    Plasmid

    #161493

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R1_gRNA_MS2

    Depositor

    Brigitte Gasser, Diethard Mattanovich

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Targeting R1 in the RIB1 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. 3xFLAG-dCas9/pTEF1p-CYC1t

    Plasmid

    #62190

    Purpose

    Expresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujii et al Biol Methods Protoc. 2022 Oct 17;7(1):bp

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Yeast

    Mutation

    human codon-optimized, D10A, H840A

    Promoter

    TEF1 promoter

    Available since

    Feb. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDB4280

    Plasmid

    #98699

    Purpose

    A Cas9-encoding plasmid containing the 5' portion of the ura4 marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-ura4 system

    Depositor

    Li-Lin Du

    Article

    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. p426-SNR52p-gRNA.csr-1.Y-SUP4t

    Plasmid

    #68060

    Purpose

    gRNA for csr-1 locus in N. crassa

    Depositor

    Christian Hong

    Article

    Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

    Insert

    gRNA for csr-1 locus
    

    Use

    CRISPR; N. crassa, fungi

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. p413TEF-PKM1

    Plasmid

    #34607

    Depositor

    Markus Ralser

    Article

    Bluemlein et al Oncotarget. 2011 May;2(5):393-400.

    Insert

    PKM1 (PKM Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. YIplac211-Mup1-mScarlet2I

    Plasmid

    #140152

    Purpose

    Plasmid for pop-in/pop-out replacement of Mup1 with Mup1-mScarlet2I

    Depositor

    Benjamin Glick

    Article

    Casler et al Elife. 2020 Jun 25;9. pii: 56844. doi: 1

    Insert

    MUP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFA6a-HyTkAX

    Plasmid

    #73898

    Purpose

    fission yeast drug counter-selectable selection marker

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Insert

    hyg-TK
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAV0620

    Plasmid

    #133506

    Purpose

    Expression of eGFP tagged PCNA to monitor DNA replication

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(pcn1)-eGFP-pcn1-terminator(nmt1)-natMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pWS2247

    Plasmid

    #123026

    Purpose

    Tet promoter (TetO(x7)-pLEU2m)

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    pTet [2]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. HO-Poly-HO

    Plasmid

    #51661

    Purpose

    yeast plasmid for integration of target sequence at HO locus

    Depositor

    David Stillman

    Article

    Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

    Type

    Empty backbone

    Use

    Yeast integration

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge