8,086 results

1. HO-Poly-HO

   Plasmid

   #51661

   Purpose

   yeast plasmid for integration of target sequence at HO locus

   Depositor

   David Stillman

   Article

   Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

   Type

   Empty backbone

   Use

   Yeast integration

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. 3xFLAG-dCas9/pTEF1p-CYC1t

   Plasmid

   #62190

   Purpose

   Expresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujii et al Biol Methods Protoc. 2022 Oct 17;7(1):bp

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Yeast

   Mutation

   human codon-optimized, D10A, H840A

   Promoter

   TEF1 promoter

   Available Since

   Feb. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDB4280

   Plasmid

   #98699

   Purpose

   A Cas9-encoding plasmid containing the 5' portion of the ura4 marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-ura4 system

   Depositor

   Li-Lin Du

   Article

   Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. YIplac211-VPS10-msGFPx3

   Plasmid

   #105274

   Purpose

   Plasmid for pop-in/pop-out replacement of Vps10 with Vps10-msGFPx3.

   Depositor

   Benjamin Glick

   Article

   Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

   Insert

   Vps10 portion (PEP1 Budding Yeast)
   

   Use

   Tags

   msGFPx3

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Jan. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSMF2

   Plasmid

   #112819

   Purpose

   Expresses wild-type Rpb1 in yeast cells

   Depositor

   Stephen Fuchs

   Article

   Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

   Insert

   RPB1 (RPO21 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Native promoter

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA6a-hygMV

   Plasmid

   #73891

   Purpose

   fission yeast drug selection marker

   Depositor

   Kazunori Tomita

   Article

   Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

   Insert

   hygMV
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   pombe TEF

   Available Since

   Nov. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXZX351

   Plasmid

   #169454

   Purpose

   pRS426-SNR52p-gRNA.MATα_option1-SUP4t; 20-bp gRNA sequence targeting MATα cassette

   Depositor

   Jef Boeke

   Article

   Xie et al G3 (Bethesda). 2018 Jan 4;8(1):173-183.

   Insert

   gRNA for MATα
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pI1-MTU-DO-G418

   Plasmid

   #160012

   Purpose

   Integrative vector for K. marxianus, targeting the LAC4 locus. G418 selection.

   Depositor

   John Morrissey

   Article

   Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

   Type

   Empty backbone

   Use

   Kluyveromyces marxianus

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   n/a

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. 3413 pBF339 Bcl-XL

   Plasmid

   #8773

   Depositor

   Stanley Korsmeyer

   Article

   Gross et al Mol Cell Biol 2000 May;20(9):3125-36.

   Insert

   Bcl-XL (Bcl2l1 Mouse)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

10. pDM291-tetR'-tup11Δ70

    Plasmid

    #41028

    Depositor

    Michael Nick Boddy

    Article

    Zilio et al Yeast. 2012 Aug 29. doi: 10.1002/yea.292

    Insert

    tetR'-tup11Δ70
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    tup11 starts at aa70

    Promoter

    Available Since

    Dec. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. p426-SNR52p-gRNA.csr-1.Y-SUP4t

    Plasmid

    #68060

    Purpose

    gRNA for csr-1 locus in N. crassa

    Depositor

    Christian Hong

    Article

    Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

    Insert

    gRNA for csr-1 locus
    

    Use

    CRISPR; N. crassa, fungi

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available Since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBS-SKII-3XHA-fSNAP-Phleomycin

    Plasmid

    #188915

    Purpose

    Integration cassette to create C-terminal fusions for fluorescent tagging

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    3XHA-fSNAP-Phleomycin
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. DdMCU

    Plasmid

    #67642

    Purpose

    DdMCU-Flag in pACT2 vector human codon optimised

    Depositor

    Vamsi Mootha

    Article

    Kovacs-Bogdan et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

    Insert

    DdMCU
    

    Use

    Tags

    Flag tag with linker

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available Since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEZY202

    Plasmid

    #18705

    Purpose

    pEG202 converted Gateway Destination vector for yeast two-hybrid bait vector

    Depositor

    Yu-Zhu Zhang

    Article

    Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

    Type

    Empty backbone

    Use

    Two-hybrid bait vector

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    July 26, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG423GAL-ccdB-EGFP

    Plasmid

    #14197

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCfB2196

    Plasmid

    #67549

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked bleMX marker, integration into S. cerevisiae XI-5 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJRoC30_Th3

    Plasmid

    #188064

    Purpose

    PROSS-designed high-redox potential laccase from Trametes hirsuta

    Depositor

    Sarel Fleishman

    Article

    Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

    Insert

    PROSS-designed high-redox potential laccase from Trametes hirsuta
    

    Use

    Tags

    S. cerevisiae evolved α-factor prepro-leader sign…

    Expression

    Yeast

    Mutation

    20 mutations, described in publication

    Promoter

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJRoC30_Tv9nL

    Plasmid

    #188069

    Purpose

    PROSS-designed high-redox potential laccase from Trametes versicolor

    Depositor

    Sarel Fleishman

    Article

    Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

    Insert

    PROSS-designed high-redox potential laccase from Trametes versicolor
    

    Use

    Tags

    S. cerevisiae evolved α-factor prepro-leader sign…

    Expression

    Yeast

    Mutation

    79 PROSS mutations, described in publication

    Promoter

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGWKS7

    Plasmid

    #139414

    Purpose

    Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mRuby3 marker.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    mRuby3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available Since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. FRP2372

    Plasmid

    #127578

    Purpose

    Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Lys2 selection marker

    Depositor

    Joerg Stelling

    Article

    Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

    Insert

    PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
    

    Use

    Synthetic Biology; Shuttle vector

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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