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Plasmid#51567PurposeConstitutively express PM (Plasma membrane) localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
UseTagsCAAX and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable sinceAug. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
YIp ADE2-HphMx
Plasmid#122916PurposeADE2-HphMx integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceOct. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUG-amdSYM-mak10TR
Plasmid#75433PurposeGenerates truncated version of MAK10 gene, which is required for dsRNA L-A propagation in S.cerevisiaeDepositorInsertsMAK10TR
ApR
amdSYM
UseTagsExpressionYeastMutationN-terminal 740 bp of MAK10 ORFPromoterNative promoter and PTEFAvailable sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKT0209
Plasmid#8730DepositorInsertyEGFP
UseTagsCodon optimized linkerExpressionYeastMutationPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLH_Scr15
Plasmid#100537PurposeCEN/ARS plasmid with expression cassettes for PhyBNT-CreN and PIF3-NLS-CreC fusion proteins for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.DepositorUseCre/Lox and Synthetic BiologyTagsCreC, CreN, and SV40 NLSExpressionYeastMutationN-terminal version of PhyB (AA 1-621)/ Mutation o…PromoterFBA1 and TDH3Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
JME4390
Plasmid#129656PurposeLEU2ex_CrisprCas9-yl_RFP: CAS9 vector with LEU2ex marker for gRNA cloning using GoldenGateDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG414GAL-ccdB
Plasmid#14143DepositorTypeEmpty backboneUseGateway destinationTagsExpressionYeastMutationPromoterAvailable sinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pH3F-GAL1
Plasmid#85775PurposeN-ICE plasmid pH3F-GAL1DepositorInsertloxP-HphMX-loxP-GAL1p-3FLAG
UseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pICE URA3
Plasmid#122882PurposeURA3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceDec. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHflu2
Plasmid#203880PurposepHI1 with insertion of ampicillin-resistance geneDepositorInsertbroad-spectrum class A beta-lactamase TEM-116
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Yeast_Target-AID3S
Plasmid#188646PurposeExpresses Target-AID3S in yeastsDepositorInsertnCas9-AID2S-UGI
UseTagsUGIExpressionYeastMutationD10A for SpCas9PromoterpGal1Available sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.028
Plasmid#184965PurposeTest effect of extending -Eco1 a1/a2 in yeastDepositorInsertEco1: RT and ncRNA(extended), a1/a2 length: 27
UseTagsExpressionYeastMutationhuman codon optimized RT, a1/a2 length extended t…PromoterGal7Available sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pE3n
Plasmid#17457Purposemodified pENTR vector for N-terminal 6x MYC tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTags6xMycExpressionBacterial, Insect, Mammalia…MutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAG303GPD-ccdB
Plasmid#14134DepositorTypeEmpty backboneUseGateway destinationTagsExpressionYeastMutationPromoterAvailable sinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
LHP398 - Ub-K48R/K63R
Plasmid#32167DepositorUseTagsExpressionYeastMutationK48R and K63RPromoterCUP1Available sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pK3FS
Plasmid#85776PurposeN-ICE plasmid pK3FSDepositorInsertloxP-KanMX-loxP-3FLAG
UseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-pro-alphaf(I)-E2-Crimson
Plasmid#117660PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpre-pro-alphaf(I)-E2-Crimson
UseTagsExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-T7-kanMX6
Plasmid#20760DepositorTypeEmpty backboneUsePcr-based c-terminal taggingTagsExpressionYeastMutationPromoterAvailable sinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mScarlet-H-mNeonGreen-kanMX (pKBJ009)
Plasmid#173456PurposeContains mScarletH-mNeonGreen tandem fluorescent protein timerDepositorInsertmScarlet-H-mNeonGreen
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
MET-HYGRO
Plasmid#218113PurposeIntegration backbone for the MET15 integration site of S. cerevisiae. Includes a HygromycinR gene and a sfGFP drop-out.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only