8,432 results
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pLyGo-Kp-1
Plasmid#163143PurposepLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-MF pre-sequence and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionYeastPromoterAOX1Available sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXZX501
Plasmid#169455PurposepRS426-SNR52p-gRNA.MATa_option3-SUP4t; 20-bp gRNA sequence targeting MATa cassetteDepositorInsertgRNA for MATa
ExpressionYeastPromoterSNR52Available sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNA0306
Plasmid#169452PurposepRS415-TEF1p-Cas9-CYC1t; Cas9 expression plasmidDepositorInsertCas9
ExpressionYeastPromoterTEF1pAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCSN075
Plasmid#166732PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS and Mxi, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-NLS-Mxi1
UseCRISPRTagsMxi1 and SV40 NLSExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
yo-msGFP2
Plasmid#160471PurposeEncodes a yeast codon optimized version of msGFP2DepositorInsertyo-msGFP2
ExpressionYeastAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cip-pAct1-Mnase-SAT1
Plasmid#162557PurposeFor the C. auris MNase control strain, the URA3 auxotrophy marker of the CIp-pACT1-3xFLAG-MNase-SV40-CYC was replaced by SAT1DepositorInsert3FLAG-Mnase-SV40
ExpressionYeastPromoterActineAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
ZJOM57
Plasmid#133656PurposeIntegration of PEX3-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast peroxisome.DepositorAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pI1-MTU-DO-URA
Plasmid#160009PurposeIntegrative vector for K. marxianus, targeting the LAC4 locus. Uracil selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusExpressionYeastPromotern/aAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMTU-DO-HPH
Plasmid#160008PurposeLow-copy expression vector for K. marxianus, hygromycin selectionDepositorTypeEmpty backboneUseKluyveromyces marxianusExpressionYeastPromotern/aAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
p416-TEF-membrane localization signal-tagBFP-wrmScarlet11-TEF terminator
Plasmid#158585PurposeExpresses membrane localized tagBFP with wrmScarlet11 C-terminal fusion in S. cerevisiaeDepositorInsertmembrane localization signal-tagBFP-wrmScarlet11
TagstagBFPExpressionYeastMutationGGGS linkerPromoterTEFAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
HsSPHK1
Plasmid#118091PurposeExpresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)DepositorInsertHomo sapiens Sphingosine Kinase 1
TagsFLAGExpressionYeastPromoterGAL1, 10Available sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAS9cd
Plasmid#141252PurposeConstitutive expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.DepositorTypeEmpty backboneUseCRISPRExpressionYeastPromoterTEF1 (Cas9), pSNR52 (gRNA)Available sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046409
Plasmid#153939PurposeYeast expression plasmid for SARS-CoV-2 S (surface glycoprotein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
TagsCleavable thrombin;StrepIIExpressionYeastMutationwtPromoterpGAL1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
p426*-SpSgH
Plasmid#136993PurposeHelper plasmid for SpCas9 gRNA cloning and expressionDepositorTypeEmpty backboneExpressionYeastPromoterSNR52Available sinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM18
Plasmid#135484PurposeAgrobacterium T-DNA with SpunH2Bpr driving hph-tdTomatoDepositorInsertSpunH2Bpr hph-tdTomato ADH1t
TagstdTomatoExpressionBacterial and YeastPromoterSpunH2BprAvailable sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGI3EM22C
Plasmid#135488PurposeAgrobacterium T-DNA with divergent H2A/Bpr driving LifeAct-tdTomato and hphDepositorInserthph SpunH2A/Bpr LifeAct-tdTomato
TagsLifeActExpressionBacterial and YeastPromoterSpunH2A/Bpr (divergent)Available sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGBW-m4046538
Plasmid#149128PurposeYeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
TagsCleavable thrombin;6xHISExpressionYeastMutationwtPromoterpTEF1Available sinceMay 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGWKS11
Plasmid#139418PurposeFluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mNeonGreen marker.DepositorInsertmNeonGreen
ExpressionYeastPromoterTEF1Available sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHRW40
Plasmid#130973PurposeHyPr plasmid with nptII markerDepositorInsertempty backbone
ExpressionYeastAvailable sinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only
