8,057 results

1. pHES836

   Plasmid

   #89195

   Purpose

   Expresses mKate2 under control of synthetic promoter containing Zif268 binding sites.

   Depositor

   Hana El-Samad

   Article

   Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

   Insert

   pZ-mKate2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pZ

   Available since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCUPI NPD-GFP

   Plasmid

   #1191

   Depositor

   Susan Lindquist

   Article

   Patino et al Science 1996 Aug 2;273(5275):622-6.

   Insert

   N-terminus of Sup35 (SUP35 Budding Yeast)
   

   Use

   Cup1 promoter

   Tags

   GFP

   Expression

   Yeast

   Mutation

   fused N-terminal Sup35 (~1-253aa) to GFP

   Promoter

   Available since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

3. pE3n

   Plasmid

   #17457

   Purpose

   modified pENTR vector for N-terminal 6x MYC tag fusion with your gene of interest

   Depositor

   Giovanna Benvenuto

   Article

   Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

   Type

   Empty backbone

   Use

   Modified gateway entry vector

   Tags

   6xMyc

   Expression

   Bacterial, Insect, Mammalia…

   Mutation

   Promoter

   Available since

   March 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBBK80 pHomL-RPL18Bp-Scarlet(rfp)-Link-VAM3-SSA1t-HomR (AmpR)

   Plasmid

   #179064

   Purpose

   Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-VAM3, a marker for the vacuole

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   RPL18Bp-Scarlet(rfp)-Link-VAM3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAV0785

   Plasmid

   #133507

   Purpose

   Expression of mCherry tagged PCNA to monitor DNA replication

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pUra4(AfeI)-p(pcn1)-mCherry-pcn1-terminator(nmt1)-natMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. LHP306 - Ub - no Lys

   Plasmid

   #32150

   Depositor

   Linda Hicke

   Article

   Terrell et al Mol Cell. 1998 Jan;1(2):193-202.

   Insert

   synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   No Lys = K6R, K11R, K27R, K29R, K33A, K48R, K63R

   Promoter

   CUP1

   Available since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pMJ240: pRS426-His-MBP-MmeFz2_Native

   Plasmid

   #205255

   Purpose

   S. cerevisiae expression vector for MmeFz2 native guide

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   MmeFz2
   

   Use

   Tags

   10xHis, MBP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. FRP1640_PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

   Plasmid

   #58440

   Purpose

   Cassette to replace yeast promoters with an inducible promoter containing 2 lexA boxes

   Depositor

   Joerg Stelling

   Article

   Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

   Insert

   PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGBW-m4046663

   Plasmid

   #153857

   Purpose

   Yeast expression plasmid for SARS-CoV-2 nsp3

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp3 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available since

   June 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pICXNHG3

    Plasmid

    #49021

    Purpose

    FX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage site

    Depositor

    Eric Geertsma, Raimund Dutzler

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Tags

    3C protease site (PreScission site), GFP, and dec…

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAG306-GPD-VMA1 chr I

    Plasmid

    #41896

    Purpose

    Yeast expression of VMA1.

    Depositor

    Dan Gottschling

    Article

    Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

    Insert

    VMA1 (VMA1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Contains silent mutation at 342A-G and L87S mutat…

    Promoter

    GPD

    Available since

    April 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDRF1 yeBFP2-T2A-mCherry

    Plasmid

    #219851

    Purpose

    equimolar expression of yeBFP2 and mCherry in budding yeast

    Depositor

    Bas Teusink

    Article

    Botman et al bioRxiv 2025.06.27.661975; doi: https://

    Insert

    yeBFP2-T2A-mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDRF1-GW yomTagBFP2-T2A-mCherry

    Plasmid

    #205493

    Purpose

    Expression of yomTagBFP2-T2A-mCherry in yeast

    Depositor

    Bas Teusink

    Article

    Botman et al bioRxiv 2025.06.27.661975; doi: https://

    Insert

    yomTagBFP2-T2A-mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGBK CUP1 Empty

    Plasmid

    #169710

    Purpose

    Yeast two-hybrid vector using a copper inducible CUP1 promoter

    Depositor

    Bruce Hamilton

    Article

    Hernandez et al bioRxiv

    Type

    Empty backbone

    Use

    Tags

    GAL4 DNA Binding Domain and Myc

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    CUP1

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDRF1-GW yomCherry-T2A-mTurquoise2

    Plasmid

    #118447

    Purpose

    Produces equimolar expression of yeast codon-optimized mCherry and mTq2

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    yomCherry-T2A-mTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMTU-DO-G418

    Plasmid

    #160007

    Purpose

    Low-copy expression vector for K. marxianus, G418 selection

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSC5GGv2

    Plasmid

    #188604

    Purpose

    pSC5 Golden-Gate Cloning Plasmid (BsaI - Site 2)

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Diatom expression

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUDE1093

    Plasmid

    #204227

    Purpose

    Episomal yeast plasmid expressing the Ercas12a

    Depositor

    Jean-Marc Daran

    Article

    Bennis et al FEMS Yeast Res. 2023 Jan 4;23:foad043. d

    Insert

    Eubacterium rectale cas12a (Ercas12a)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    codon optimized for S. cerevisiae

    Promoter

    Saccharomyces cerevisiae PGK1

    Available since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMJA065

    Plasmid

    #128517

    Purpose

    Expresses Ber e 1 in Pichia

    Depositor

    Marcos Alcocer

    Article

    Alcocer et al J Mol Biol. 2002 Nov 15;324(1):165-75.

    Insert

    Ber e 1 a allergenic 2S albumin from Brazil nut
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPS1622

    Plasmid

    #8854

    Depositor

    Pamela Silver

    Article

    Prinz et al J Cell Biol. 2000 Aug 7. 150(3):461-74.

    Insert

    Sec63-sGFP (SEC63 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    sGFP=S65T V163A mutant of GFP

    Promoter

    Available since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only