8,418 results

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pHES854

Plasmid

#89197

Purpose

Expresses mKate2 under control of TDH3 constitutive promoter.

Depositor

Hana El-Samad

Publication

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pTDH3-mKate2

Expression

Yeast

Promoter

TDH3

Available since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pOP459

Plasmid

#85933

Purpose

Encodes the Anti-Ebola 2G4 monoclonal antibody. Chimeric - mouse variable regions, human constant regions.

Depositor

Timothy Lu

Publication

Purcell et al ACS Synth Biol. 2017 Aug 17. doi: 10.102

Insert

Anti-Ebola 2G4 monoclonal antibody

Expression

Yeast

Promoter

AOX1

Available since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pHES820

Plasmid

#89190

Purpose

Expresses YFP under control of GAL1 promoter.

Depositor

Hana El-Samad

Publication

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pGAL1-YFP

Expression

Yeast

Promoter

GAL1

Available since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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YEp112-CUP1-His-Smt3

Plasmid

#99537

Purpose

Yeast episomal vector for expression of His-tagged Smt3 (SUMO); TRP1 marker

Depositor

Helle Ulrich

Publication

Ulrich et al Methods Mol Biol. 2009;497:81-103. doi:

Insert

SMT3 (SMT3 Budding Yeast)

Tags

7xHis

Expression

Yeast

Promoter

CUP1

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pIMVAu1

Plasmid

#98301

Purpose

Enhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of galactose-inducible promoters; integrated into genome HMG2 locus; overexpressing HMG2 K6R.

Depositor

Claudia Vickers

Publication

Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

Insert

HMG2(-309,-153)-HIS3-TEFM1ACS2>TACS2-PGAL2> EfmvaE >TEBS1-PGAL7>HMG2K6A(1,292)

Expression

Yeast

Available since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pPTK002-2-pGAP

Plasmid

#84961

Purpose

Type 2, pGAP, Glyceraldehyde-3-phosphate dehydrogenase promoter

Depositor

Volker Sieber

Publication

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Glyceraldehyde-3-phosphate dehydrogenase promoter

Use

Synthetic Biology

Expression

Yeast

Mutation

BsaI site removed (1805 c to g)

Promoter

Non

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pPTK006-3a-αMF no EAEA

Plasmid

#84965

Purpose

Type 3a, α-mating factor no EAEA - secretion signal

Depositor

Volker Sieber

Publication

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

α-mating factor no EAEA secretion tag

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pPICZA_CeTRIC-B1_Del

Plasmid

#87344

Purpose

Inducible expression of CeTRIC-B1 in P.Pichia strains

Depositor

Zhenfeng Liu

Publication

Yang et al Nature. 2016 Oct 27;538(7626):537-541. d

Insert

TRIC-B1

Tags

polyhistidine

Expression

Yeast

Mutation

Δ248-297

Promoter

AOX1

Available since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pSJ1726 (pFA6-NATMX-CDC42pr-KAR2SS-GFP1-10)

Plasmid

#86421

Purpose

N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter and KAR2 signal sequence

Depositor

Sue Jaspersen

Publication

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

CDC42pr-KAR2SS-GFP1-10

Tags

GFP1-10

Expression

Yeast

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSJ1679 (pRS315-NOP1pr-GFP11-PUS1)

Plasmid

#86414

Purpose

nuclear split GFP11 reporter without mCherry

Depositor

Sue Jaspersen

Publication

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

NOP1pr-GFP11-PUS1

Tags

GFP11 and mCherry

Expression

Yeast

Promoter

NOP1pr

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pK3FS-GAL1

Plasmid

#85778

Purpose

N-ICE plasmid pK3FS-GAL1

Depositor

Scott Briggs

Publication

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-GAL1p-3FLAG

Use

Cre/Lox

Expression

Yeast

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pK3FS-CYC1

Plasmid

#85779

Purpose

N-ICE plasmid pK3FS-CYC1

Depositor

Scott Briggs

Publication

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-CYC1p-3FLAG

Use

Cre/Lox

Expression

Yeast

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2+ cMyc-oDam_f_GFPoNLS

Plasmid

#85818

Purpose

iDamID plasmid. To express transiently the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

Depositor

Joachim Wittbrodt

Publication

Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

Insert

cMyc oDam_f_GFPoNLS

Tags

cMyc and oNLS (optimized nuclear localization sig…

Expression

Bacterial, Insect, Mammalia…

Mutation

L122A. Codon optimization compatible to work in M…

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCfB3035(X-4 MarkerFree)

Plasmid

#73273

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-4 (Chr X: 236336..237310)

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB3042(gRNA X-4)

Plasmid

#73284

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-2

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pNXRVa-HACre

Plasmid

#78398

Purpose

constitutive Cre expression vector

Depositor

Kazunori Tomita

Publication

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xHA-nls-Cre
kanMX6

Expression

Yeast

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-HyTkAX

Plasmid

#73898

Purpose

fission yeast drug counter-selectable selection marker

Depositor

Kazunori Tomita

Publication

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Insert

hyg-TK

Expression

Yeast

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pFA6a-FHyTkAX

Plasmid

#73901

Purpose

fission yeast drug counter-selectable selection marker

Depositor

Kazunori Tomita

Publication

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Insert

hyg-TK

Expression

Yeast

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-neoCV

Plasmid

#73889

Purpose

fission yeast drug selection marker

Depositor

Kazunori Tomita

Publication

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Insert

neoCV

Expression

Bacterial, Mammalian, and Y…

Promoter

CMV

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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GTT-g

Plasmid

#81109

Purpose

Gene Tagging vector TRP/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)

Depositor

Serge Pelet

Publication

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Insert

sfGFP

Use

Cre/Lox

Tags

sfGFP

Expression

Yeast

Available since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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