8,008 results

1. pGBW-m4046708

   Plasmid

   #149173

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp4

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. BB3rN_14*

   Plasmid

   #98550

   Purpose

   empty BB3 for insertion and overexpression of 1 gene in P. pastoris

   Depositor

   Michael Sauer, Brigitte Gasser, Diethard Mattanovich

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pABY-c46

   Plasmid

   #201774

   Purpose

   Expresses mNeonGreen-tagged NbALFA under TEF1 promoter in yeast

   Depositor

   Saravanan Palani

   Article

   Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

   Insert

   NbALFA
   

   Use

   Tags

   40aa Linker, mNeonGreen

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBEVY-4420-mCherry

   Plasmid

   #218828

   Purpose

   Yeast surface display of an anti-fluorescene scFv and intracellular expression of mCherry

   Depositor

   Yongku Cho

   Article

   Arbaciauskaite et al Protein Eng Des Sel. 2023 Jan 21;36:gzad

   Insert

   4-4-20 scFv
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1-10

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBEVY-pT231 scFv-GFP

   Plasmid

   #218827

   Purpose

   Yeast surface display of a phospho-tau pT231 scFv and intracellular expression of GFP

   Depositor

   Yongku Cho

   Article

   Arbaciauskaite et al Protein Eng Des Sel. 2023 Jan 21;36:gzad

   Insert

   pT231 tau scFv
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1-10

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. p404-Grx5-GFP1-10

   Plasmid

   #91952

   Purpose

   Expresses GFP1-10 in the mitochondrial matrix

   Depositor

   Rong Li

   Article

   Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

   Insert

   Grx5-GFP1-10
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAP

   Available since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGFP-ATG13(315)

   Plasmid

   #122072

   Purpose

   Expressing GFP-Atg13

   Depositor

   Daniel Klionsky

   Article

   Chew et al Autophagy. 2015;11(6):891-905. doi: 10.1

   Insert

   ATG13
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRS402-nicastrin/APH-1a-HA

   Plasmid

   #207804

   Purpose

   Yeast integrative vector for expression of Nicastrin and APH-1a-HA

   Depositor

   Dieter Langosch

   Article

   Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

   Insert

   Gamma-secretase subunits nicastrin and Aph1a
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAG425GPD-ccdB-DsRed

   Plasmid

   #14370

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   DsRed

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pICE URA3

    Plasmid

    #122882

    Purpose

    URA3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFA6a-mScarlet-H-mNeonGreen-kanMX (pKBJ009)

    Plasmid

    #173456

    Purpose

    Contains mScarletH-mNeonGreen tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mScarlet-H-mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    no promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFA6a-link-yomKeima-Kan

    Plasmid

    #44902

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mKeima with Kan selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomKeima
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. YIPlac204TKC-E2-Crimson-HDEL

    Plasmid

    #38771

    Depositor

    Benjamin Glick

    Article

    Strack et al Biochemistry. 2009 Sep 8;48(35):8279-81.

    Insert

    E2-Crimson
    

    Use

    Tags

    Linker peptide and yeast ER retention signal sequ…

    Expression

    Yeast

    Mutation

    Promoter

    TPI1

    Available since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. FRP1639_PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

    Plasmid

    #58439

    Purpose

    Cassette to replace yeast promoters with an inducible promoter containing 1 lexA box

    Depositor

    Joerg Stelling

    Article

    Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

    Insert

    PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. M4755 KanMX::LEU2 Disruptor Converter

    Plasmid

    #51685

    Purpose

    Yeast marker swap plasmid for converting KanMX3 to LEU2

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    LEU2 (LEU2 Budding Yeast)
    

    Use

    Yeast marker swap

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG304GAL-ccdB

    Plasmid

    #14135

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pETconNK-TEM1(S70A,D179G)

    Plasmid

    #81166

    Purpose

    pETconNK with TEM1(S70A,D179G) beta-lactamase

    Depositor

    Timothy Whitehead

    Article

    Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

    Insert

    TEM-1 beta-lactamase
    

    Use

    Tags

    c-myc

    Expression

    Bacterial and Yeast

    Mutation

    Deleted the sec transport tag. Plasmid encodes H2…

    Promoter

    GAL1 and gal1

    Available since

    Feb. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYX122-mtGFP

    Plasmid

    #45048

    Depositor

    Benedikt Westermann

    Article

    Westermann et al Yeast. 2000 Nov;16(15):1421-7.

    Insert

    mtGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TPI

    Available since

    May 17, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pCEV-G2-Ph

    Plasmid

    #46816

    Purpose

    TEF1 and PGK1 promoter controlled expression cassettes with phleomycin resistance for yeast transformation

    Depositor

    Claudia Vickers, Lars Nielsen

    Article

    Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

    Type

    Empty backbone

    Use

    Tags

    FLAG and c-myc

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 and PGK1

    Available since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGI3EM09

    Plasmid

    #135482

    Purpose

    Agrobacterium T-DNA with SpunHsp70pr driving hph-GFP

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    SpunHsp70pr hph-GFP ADH1t
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpunHsp70pr

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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