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pCfB2191

Plasmid

#67538

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked KlURA3 marker, integration into S. cerevisiae XII-1 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Publication

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Expression

Yeast

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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LD211

Plasmid

#69183

Purpose

Integration of 2GFP tag at Atg11 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Publication

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg11-2EGFP

Tags

2xEGFP

Expression

Yeast

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pRG215

Plasmid

#64525

Purpose

Centromeric E. coli/S. cerevisiae shuttle vector

Depositor

Joerg Stelling

Publication

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Type

Empty backbone

Expression

Yeast

Available since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJZC523

Plasmid

#62320

Purpose

sgRNA for yeast cells

Depositor

Wendell Lim, Stanley Qi

Publication

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA

Expression

Yeast

Promoter

SNR52

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pESC-URA-hPOLISc

Plasmid

#60819

Purpose

to express human Pol iota recoded to yeast codons in yeast

Depositor

Youri Pavlov

Publication

Makarova et al PLoS One. 2011 Jan 31;6(1):e16612. doi:

Insert

POLI (POLI Human)

Tags

c-myc

Expression

Yeast

Mutation

optimized to yeast codon usage

Promoter

GAL1

Available since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pPL5628_TEF1*-Cre_MET15

Plasmid

#60933

Purpose

Constitutive expression vector for Cre-recombinase containing MET15 selection marker.

Depositor

Robert Piper

Publication

MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

Insert

Cre

Tags

Driven from a mutant TEF1 promoter with 0.16x act…

Expression

Yeast

Promoter

mutant TEF1

Available since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pYIAL31

Plasmid

#60807

Purpose

used to make mutations in yeast POL1

Depositor

Thomas Kunkel, Youri Pavlov

Publication

Pavlov et al Genetics. 2001 Sep;159(1):47-64.

Insert

Pol1 (POL1 Budding Yeast)

Expression

Yeast

Mutation

N-terminal part only

Available since

Feb. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pDDGFP2_leu2d_NRT1.1

Plasmid

#58332

Purpose

Expresses Arabidopsis thaliana NRT1.1 in Saccharomyces cells as a C-terminal GFP his tagged protein

Depositor

Simon Newstead

Publication

Parker et al Nature. 2014 Mar 6;507(7490):68-72. doi:

Insert

NRT1.1 (NRT1.1 Mustard Weed)

Tags

GFP-His

Expression

Yeast

Promoter

GAL1

Available since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only

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PhyB-mCherry-SNF7

Plasmid

#51574

Purpose

Constitutively express endosome localized PhyB in Saccharomyces cerevisiae

Depositor

Chao Tang

Publication

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PhyB (PHYB Mustard Weed)

Tags

SNF7 and mCherry

Expression

Yeast

Mutation

1-908aa only

Promoter

ADH1pr

Available since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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M4757 KanMX::TRP1 Disruptor Converter

Plasmid

#51687

Purpose

Yeast marker swap plasmid for converting KanMX3 to TRP1

Depositor

David Stillman

Publication

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

TRP1 (TRP1 Budding Yeast)

Expression

Yeast

Available since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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M2660 ura3::LYS2 Disruptor Converter

Plasmid

#51678

Purpose

Yeast marker swap plasmid for converting URA3 to LYS2

Depositor

David Stillman

Publication

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

LYS2 (LYS2 Budding Yeast)

Use

Yeast marker swap

Expression

Yeast

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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pICXC3H

Plasmid

#49022

Purpose

FX cloning Pichia pastoris expression vector with AOX1 promoter and C-terminal 3C protease cleavage site and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Publication

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Yeast

Promoter

AOX1

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoPS-CFP2-Kan

Plasmid

#44948

Purpose

Yeast tagging vector to create gene fusion with yeast optimized photoconvertible CFP2 with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoPS-CFP2

Expression

Yeast

Available since

Oct. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoPS-CFP2-SpHis5

Plasmid

#44853

Purpose

Yeast tagging vector to create gene fusion with yeast optimized photoconvertible CFP2 with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoPS-CFP2

Expression

Yeast

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoLSS-mKate2-SpHis5

Plasmid

#44848

Purpose

Yeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoLSS-mKate2

Expression

Yeast

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomTagBFP-SpHis5

Plasmid

#44838

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mTagBFP with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomTagBFP

Expression

Yeast

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pXP732

Plasmid

#46058

Depositor

Nancy DaSilva

Publication

Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

Type

Empty backbone

Expression

Yeast

Promoter

CUP1

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pXP742

Plasmid

#46060

Depositor

Nancy DaSilva

Publication

Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

Type

Empty backbone

Expression

Yeast

Promoter

ADH2

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pXP821

Plasmid

#46063

Depositor

Nancy DaSilva

Publication

Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

Type

Empty backbone

Expression

Yeast

Promoter

GAL1

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pXP822

Plasmid

#46064

Depositor

Nancy DaSilva

Publication

Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

Type

Empty backbone

Expression

Yeast

Promoter

GAL1

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

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