8,223 results
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Plasmid#228293PurposeConstitutive mutant PhlTA expression with strong promoterDepositorInsertphlTA mutant
UseSynthetic BiologyTagsThree copies of VP16 (VP48)-Nuclear localization …ExpressionYeastMutationE41GPromoterScPGK1 promoterAvailable SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1035
Plasmid#228300PurposeConstitutive expression of LuxTA mutant with strong promoterDepositorInsertluxTA mutant
UseSynthetic BiologyTagsNuclear localization signal-Three copies of VP16 …ExpressionYeastMutationS116Y, W201RPromoterKpGAPDH promoterAvailable SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMM0646
Plasmid#129007PurposeIntegration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 markerDepositorInsertKanR-ColE1 URA 5'-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
ExpressionYeastAvailable SinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVG122
Plasmid#211765PurposeExpresses strongLOV under PGK1pr. StrongLOV is a variant of optogenetic transcription factor EL222 that is more sensitive than EL222 to blue light.DepositorInsertstrongLOV
UseFor single-copy integration (if cut with pmei)TagsSV40 NLS and VP16ExpressionYeastMutationcompared to EL222, strongLOV contains Glu84Asp mu…Available SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS263
Plasmid#122379PurposeCRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -141 bp upstream of HSP90 start codonDepositorInsertHSP90
UseCRISPRExpressionYeastAvailable SinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
p413TEF-PKM2
Plasmid#34608DepositorAvailable SinceJan. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pE5c
Plasmid#17465Purposemodified pENTR vector for C-terminal ECFP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsECFPExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mEGFP-KanMX6
Plasmid#87023PurposeTag S. pombe genes in C terminal with mEGFPDepositorInsertmEGFP
ExpressionBacterial and YeastAvailable SinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST-DHFR F[1,2]-N (TRP1)
Plasmid#177797PurposeGateway destination vector to insert genes of interest having a N-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.DepositorTypeEmpty backboneTagsDHFR F[1,2]ExpressionYeastPromoterADH1Available SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHpgRNA13
Plasmid#160127PurposeTo express a sgRNA with constant targeting ADE2 by RNA pol II promoter PTEF1DepositorInsertPTEF1-HH-gRNA-ADE2- HDV-TAMO
ExpressionYeastAvailable SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-CHS3-msGFPx3
Plasmid#105257PurposePlasmid for pop-in/pop-out replacement of Chs3 with Chs3-msGFPx3.DepositorAvailable SinceFeb. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMJ123: pRS426-His-MBP-SpuFz1_PSP1
Plasmid#205256PurposeS. cerevisiae expression vector for SpuFz1 reprogrammed guide (targeting PSP1)DepositorInsertSpuFz1
Tags10xHis, MBPExpressionYeastAvailable SinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNB2176
Plasmid#113395PurposeTy3-IN-GFP; alpha factor inducibleDepositorInsertTy3 with integrase-green fluorescent protein
TagsIN-GFPExpressionYeastAvailable SinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
FRP2372
Plasmid#127578PurposeRepressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Lys2 selection markerDepositorInsertPRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
UseSynthetic Biology; Shuttle vectorExpressionYeastAvailable SinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJRoC30_VP11H
Plasmid#188063PurposePROSS-designed versatile peroxidase 11HDepositorInsertPROSS-designed versatile peroxidase from Pleurotus ostreatus
TagsS. cerevisiae native α-factor prepro-leader signa…ExpressionYeastMutation38 PROSS mutations, described in publicationAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
ZJOM70
Plasmid#133658PurposeIntegration of ERG6-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast lipid droplet.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ymVenus
Plasmid#118454PurposeOverexpression of yeast codon-optimized mVenusDepositorInsertymVenus
ExpressionYeastAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pI1-MTU-DO-HPH
Plasmid#160014PurposeIntegrative vector for K. marxianus, targeting the LAC4 locus. Hygromycin selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusExpressionYeastPromotern/aAvailable SinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG306-GPD-ccdB chr I
Plasmid#41894DepositorTypeEmpty backboneExpressionYeastPromoterGPDAvailable SinceFeb. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pYZ157
Plasmid#98413PurposePombe Expression Vector - nmt81 promoter with Sp.ura4 markerDepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only