8,086 results

1. pUC-TALO8

   Plasmid

   #83409

   Purpose

   TRP1-ARS1-8xLacO (TALO8) minichromosome system for histone purification in yeast

   Depositor

   Toshi Tsukiyama

   Article

   Unnikrishnan et al Nat Struct Mol Biol. 2010 Apr;17(4):430-

   Insert

   TRP-ARS-8xLacO
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Please see depositor comments below

   Promoter

   TRP1

   Available Since

   Nov. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFA6a-link-yomApple-Kan

   Plasmid

   #44957

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized mApple with Kan selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yomApple
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMET17p-CRU313

   Plasmid

   #131163

   Purpose

   For ScMET17 promoter regulated expression of Cub-R-URA3 fused bait proteins, centromeric ARS plasmid, HIS3 complements the histidine auxotrophy of S. cerevisiae his mutant

   Depositor

   Nils Johnsson

   Article

   Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

   Type

   Empty backbone

   Use

   Tags

   CRU

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Sept. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pESC/mCitrine

   Plasmid

   #117996

   Purpose

   Expression of mCitrine controlled by the Gal1 promoter

   Depositor

   Mathias Pribil

   Article

   Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

   Insert

   mCitrine
   

   Use

   Yeast/bacteria binary vector

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Gal1

   Available Since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. p404-Grx5-GFP1-10

   Plasmid

   #91952

   Purpose

   Expresses GFP1-10 in the mitochondrial matrix

   Depositor

   Rong Li

   Article

   Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

   Insert

   Grx5-GFP1-10
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAP

   Available Since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS315-ADH700p-yeCherry-p150-yeGFP-CYCt

   Plasmid

   #194519

   Purpose

   Dual fluorescence reporter for studying protein degradation

   Depositor

   Tommer Ravid

   Article

   Mashahreh et al Nat Commun. 2022 Dec 8;13(1):7588. doi:

   Insert

   TIF4631 leader sequence
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGBT9-Hook3

   Plasmid

   #198525

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-Hook3 fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

   Insert

   Hook3 (HOOK3 Human)
   

   Use

   Tags

   GAL4-DNA binding domain (BD) fragment

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available Since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. EZ-L580

   Plasmid

   #173023

   Purpose

   Encodes OptoAMP4 circuit

   Depositor

   Jose Avalos

   Article

   Zhao et al ACS Synth Biol. 2021 May 21;10(5):1143-1

   Insert

   VP16-EL222
   

   Use

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   A79Q

   Promoter

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXNgate22-1HA

   Plasmid

   #105077

   Purpose

   Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

   Depositor

   Wolf Frommer

   Article

   Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

   Type

   Empty backbone

   Use

   Tags

   NubG

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDRF1 yeBFP2-T2A-mCherry

    Plasmid

    #219851

    Purpose

    equimolar expression of yeBFP2 and mCherry in budding yeast

    Depositor

    Bas Teusink

    Article

    Botman et al bioRxiv 2025.06.27.661975; doi: https://

    Insert

    yeBFP2-T2A-mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDRF1-GW yomTagBFP2-T2A-mCherry

    Plasmid

    #205493

    Purpose

    Expression of yomTagBFP2-T2A-mCherry in yeast

    Depositor

    Bas Teusink

    Article

    Botman et al bioRxiv 2025.06.27.661975; doi: https://

    Insert

    yomTagBFP2-T2A-mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGBK CUP1 Empty

    Plasmid

    #169710

    Purpose

    Yeast two-hybrid vector using a copper inducible CUP1 promoter

    Depositor

    Bruce Hamilton

    Article

    Hernandez et al bioRxiv

    Type

    Empty backbone

    Use

    Tags

    GAL4 DNA Binding Domain and Myc

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    CUP1

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDRF1-GW yomCherry-T2A-mTurquoise2

    Plasmid

    #118447

    Purpose

    Produces equimolar expression of yeast codon-optimized mCherry and mTq2

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    yomCherry-T2A-mTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. HO-pMET-poly-KanMX4-HO

    Plasmid

    #51665

    Purpose

    yeast plasmid for integration of target sequence at HO locus, Kan selection, pMET inducible promoter

    Depositor

    David Stillman

    Article

    Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

    Type

    Empty backbone

    Use

    Yeast integration

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUDE1093

    Plasmid

    #204227

    Purpose

    Episomal yeast plasmid expressing the Ercas12a

    Depositor

    Jean-Marc Daran

    Article

    Bennis et al FEMS Yeast Res. 2023 Jan 4;23:foad043. d

    Insert

    Eubacterium rectale cas12a (Ercas12a)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    codon optimized for S. cerevisiae

    Promoter

    Saccharomyces cerevisiae PGK1

    Available Since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMJA065

    Plasmid

    #128517

    Purpose

    Expresses Ber e 1 in Pichia

    Depositor

    Marcos Alcocer

    Article

    Alcocer et al J Mol Biol. 2002 Nov 15;324(1):165-75.

    Insert

    Ber e 1 a allergenic 2S albumin from Brazil nut
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. ZJOM11

    Plasmid

    #133637

    Purpose

    Integration of GFP-TLG1 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast late golgi/early endosome.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    pCUP1-GFP-Tlg1 (TLG1 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVG47

    Plasmid

    #164705

    Purpose

    yEVenus under the control of tetOpr

    Depositor

    Sahand Jamal Rahi

    Article

    Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

    Insert

    tetOpr - yEVenus - CLN2 PEST - ADH1t
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. M4755 KanMX::LEU2 Disruptor Converter

    Plasmid

    #51685

    Purpose

    Yeast marker swap plasmid for converting KanMX3 to LEU2

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    LEU2 (LEU2 Budding Yeast)
    

    Use

    Yeast marker swap

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMM0638

    Plasmid

    #129005

    Purpose

    Integration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    5' URA3 -pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'- KanR-ColE1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge