8,057 results

1. WT (DVD)

   Plasmid

   #184316

   Purpose

   To study dynamics of G-actin using NMR spectroscopy.

   Depositor

   Michael Rosen

   Article

   Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

   Insert

   Act5C (Act5C Fly)
   

   Use

   Tags

   Hexahistidine followed by B-thymosin

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFA6a-link-yomKeima-Kan

   Plasmid

   #44902

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized mKeima with Kan selection

   Depositor

   Kurt Thorn, Wendell Lim

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yomKeima
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBBK90 pHomL-TEF2p-Scarlet(rfp)-Link-2xPH-SSA1t-HomR (AmpR)

   Plasmid

   #179074

   Purpose

   Vector containing a yeast integration cassette for expressing TEF2p-Scarlet(rfp)-Link-2xPH, an overexpressed (bright) marker for the plasma membrane

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   TEF2p-Scarlet(rfp)-Link-2xPH
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAG426GAL-ccdB-DsRed

   Plasmid

   #14371

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   DsRed

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. Cip-pAct1-Mnase-SAT1

   Plasmid

   #162557

   Purpose

   For the C. auris MNase control strain, the URA3 auxotrophy marker of the CIp-pACT1-3xFLAG-MNase-SV40-CYC was replaced by SAT1

   Depositor

   Adnane Sellam

   Article

   Tebbji et al mSphere. 2020 Oct 14;5(5). pii: 5/5/e006

   Insert

   3FLAG-Mnase-SV40
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Actine

   Available since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. JME4390

   Plasmid

   #129656

   Purpose

   LEU2ex_CrisprCas9-yl_RFP: CAS9 vector with LEU2ex marker for gRNA cloning using GoldenGate

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. p406 – TDH3-Elo3-GFP11-Ura

   Plasmid

   #177734

   Purpose

   ER protein Elo3 with a C-terminal, an HA tag and the eleventh β-strand of GFP in yeast plasmid with URA marker

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   Elo3 (ELO3 Budding Yeast)
   

   Use

   Tags

   GFP11 and HDEL

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAG425GPD-EGFP-ccdB

   Plasmid

   #14322

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAG304GAL-ccdB

   Plasmid

   #14135

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. M3925 trp1::KanMX3 Disruptor Converter

    Plasmid

    #51681

    Purpose

    Yeast marker swap plasmid for converting Trp1 to KanMX3

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    KanMX3
    

    Use

    Yeast marker swap

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. FRP1639_PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

    Plasmid

    #58439

    Purpose

    Cassette to replace yeast promoters with an inducible promoter containing 1 lexA box

    Depositor

    Joerg Stelling

    Article

    Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

    Insert

    PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFA6a-link-yoLSS-mKate2-Kan

    Plasmid

    #44943

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with Kan selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoLSS-mKate2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAG426GPD-Cerulean-ccdB

    Plasmid

    #14420

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Cerulean

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCEV-G2-Ph

    Plasmid

    #46816

    Purpose

    TEF1 and PGK1 promoter controlled expression cassettes with phleomycin resistance for yeast transformation

    Depositor

    Claudia Vickers, Lars Nielsen

    Article

    Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

    Type

    Empty backbone

    Use

    Tags

    FLAG and c-myc

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 and PGK1

    Available since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCfB3052(gRNA X-4, XI-3, XII-5)

    Plasmid

    #73294

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-4, XI-3, and XII-5

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAG416GAL10-HRV-B14_3C

    Plasmid

    #203466

    Purpose

    Expresses HRV-B14 3C protease from a GAL10 promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Article

    Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

    Insert

    HRV-B14 3C protease (HRVBgp1 )
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFA6a-mScarlet-H-mNeonGreen-kanMX (pKBJ009)

    Plasmid

    #173456

    Purpose

    Contains mScarletH-mNeonGreen tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mScarlet-H-mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    no promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPS2044

    Plasmid

    #1375

    Depositor

    Pamela Silver

    Article

    Brodsky et al RNA 2000 Dec;6(12):1737-49.

    Insert

    RPL25-U1A-PGK1 3' UTR (RPL25 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

19. pYES2-Ub-R-GFP

    Plasmid

    #11953

    Depositor

    Nico Dantuma

    Article

    Heessen et al FEBS Lett. 2003 Dec 4. 555(2):397-404.

    Insert

    Ub-R-GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Ubiquitin fused to N-terminus of GFP. Arginine at…

    Promoter

    Available since

    May 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pICE URA3

    Plasmid

    #122882

    Purpose

    URA3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge