8,086 results

1. pJZC620

   Plasmid

   #62282

   Purpose

   Expresses dCas9, MCP-VP64, and PCP-VP64 in Yeast cells

   Depositor

   Stanley Qi, Wendell Lim

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Inserts

   MCP-VP64
   PCP-VP64
   dCas9
   

   Use

   Tags

   VP64

   Expression

   Yeast

   Mutation

   Nuclease activity has been inactivated by mutatio…

   Promoter

   pAdh and pTdh3

   Available Since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRS406-PHO5-GFP-OSBP PH domain

   Plasmid

   #58840

   Purpose

   Expresses GFP-Tagged OSBP PH domain in yeast

   Depositor

   Tim Levine, Sean Munro

   Article

   Levine et al Curr Biol. 1998 Jun 18;8(13):729-39.

   Insert

   Oxysterol-binding Protein PH domain (OSBP Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   PHO5

   Available Since

   Jan. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pYC34

   Plasmid

   #63915

   Purpose

   Recipient overexpressing vector to construct mCherry tagged fusions

   Depositor

   Irene Castano Navarro

   Article

   Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

   Type

   Empty backbone

   Use

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   Promoter

   TEF1 (Saccharomyces cerevisiae)

   Available Since

   April 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDS1885

   Plasmid

   #177341

   Purpose

   Expresses Aequorin in Candida albicans using the dominant marker SAT1

   Depositor

   Dominique Sanglard

   Article

   Sanglard et al J Fungi (Basel). 2021 Apr 20;7(4). pii:

   Insert

   Aequorin
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ACT1

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUDP013

   Plasmid

   #103873

   Purpose

   Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Ogataea (para)polymorpha

   Depositor

   Jean-Marc Daran

   Article

   Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

   Insert

   HH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ScTDH3

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNXgate32-3HA

   Plasmid

   #105076

   Purpose

   Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

   Depositor

   Wolf Frommer

   Article

   Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

   Type

   Empty backbone

   Use

   Tags

   3xHA and NubG

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFA6a-12Pk-kanMX6

   Plasmid

   #49176

   Purpose

   genomic 12Pk epitope tagging in S. pombe

   Depositor

   Eishi Noguchi

   Article

   Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

   Type

   Empty backbone

   Use

   Tags

   12Pk

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAV10.KN

   Plasmid

   #63209

   Purpose

   pCACS backbone (Amp), cloNAT, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGAL-flp(H305L)-NAT

   Plasmid

   #160380

   Purpose

   overexpression of Flp recombinase step-arrest mutant in yeast

   Depositor

   John McCusker

   Insert

   FLP1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   H305L

   Promoter

   GAL

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFTK020

    Plasmid

    #171292

    Purpose

    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    PalcA syn_NoCrea (ANIA_08979) promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Promoter

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pXP741

    Plasmid

    #46059

    Depositor

    Nancy DaSilva

    Article

    Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH2

    Available Since

    Sept. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pINER5

    Plasmid

    #185895

    Purpose

    In vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).

    Depositor

    Claudia Vickers

    Insert

    PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PSk.GAL2>YFAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available Since

    Aug. 25, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pDDGFP2_leu2d_NRT1.1

    Plasmid

    #58332

    Purpose

    Expresses Arabidopsis thaliana NRT1.1 in Saccharomyces cells as a C-terminal GFP his tagged protein

    Depositor

    Simon Newstead

    Article

    Parker et al Nature. 2014 Mar 6;507(7490):68-72. doi:

    Insert

    NRT1.1 (NRT1.1 Mustard Weed)
    

    Use

    Tags

    GFP-His

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    Aug. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pH

    Plasmid

    #64178

    Purpose

    harbours marker gene HIS3

    Depositor

    Markus Ralser

    Article

    Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGBW-m4046664

    Plasmid

    #149224

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pGBW-m4046660

    Plasmid

    #149214

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp2

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp2 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pGBW-m4046689

    Plasmid

    #149212

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp6

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pXP320

    Plasmid

    #26838

    Depositor

    Suzanne Sandmeyer, Nancy DaSilva

    Article

    Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Dec. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFA6a-GBP-hphMX6

    Plasmid

    #105159

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Davidson et al Mol Biol Cell. 2015 Feb 1;26(3):453-66.

    Insert

    GBP-hphMX6
    

    Use

    Tags

    GBP

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Feb. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPEE47

    Plasmid

    #139426

    Purpose

    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mNeonGreen ORF and a flexible linker at the 5' end.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available Since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge