7,933 results

1. LT2_14_GlyOx

   Plasmid

   #177711

   Purpose

   Plasmid for genome integration in X2 site expressing GlyRNA and OxPro (biosensors for glycolytic flux and oxidative stress)

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

   Inserts

   pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
   pTEFmut8-mCherry-tADH1
   pTRX2_5xUAS-ymYPET-tCYC1
   

   Use

   Used as donor dna in genome integration after lin…

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pE2c

   Plasmid

   #17462

   Purpose

   modified pENTR vector for C-terminal 3x HA tag fusion with your gene of interest

   Depositor

   Giovanna Benvenuto

   Article

   Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

   Type

   Empty backbone

   Use

   Modified gateway entry vector

   Tags

   3xHA

   Expression

   Bacterial, Insect, Mammalia…

   Mutation

   Promoter

   Available since

   March 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK1

   Plasmid

   #111722

   Purpose

   to express human BLM protein in yeast cells

   Depositor

   Ian Hickson

   Article

   Karow et al J Biol Chem. 1997 Dec 5;272(49):30611-4.

   Insert

   BLM (BLM Human)
   

   Use

   Tags

   hexahistidine

   Expression

   Yeast

   Mutation

   a C-terminal hexahistidine tag was added, a yeast…

   Promoter

   GAL1

   Available since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pML104-NatMx3

   Plasmid

   #83477

   Purpose

   Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains NatMX3 marker for yeast transformation.

   Depositor

   Patricia Wittkopp

   Insert

   NatMx3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pYDS2.0_AT118-L

   Plasmid

   #222847

   Purpose

   Yeast-display plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivity

   Depositor

   Andrew Kruse

   Article

   Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

   Insert

   AT118-L
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDRf1-GW

   Plasmid

   #36026

   Depositor

   Dominique Loque, Wolf Frommer

   Article

   Loque et al Nature. 2007 Mar 8;446(7132):195-8. Epub

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 26, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIMVAu1

   Plasmid

   #98301

   Purpose

   Enhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of galactose-inducible promoters; integrated into genome HMG2 locus; overexpressing HMG2 K6R.

   Depositor

   Claudia Vickers

   Article

   Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

   Insert

   HMG2(-309,-153)-HIS3-TEFM1ACS2>TACS2-PGAL2> EfmvaE >TEBS1-PGAL7>HMG2K6A(1,292)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFA6a-yomRuby3-SpHIS5

   Plasmid

   #204287

   Purpose

   For C-terminal tagging using yeast optimized mRuby3

   Depositor

   Linda Huang

   Article

   Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

   Insert

   pFA6-yomRuby3-SpHIS5
   

   Use

   Tags

   yomRuby3

   Expression

   Yeast

   Mutation

   Promoter

   none

   Available since

   July 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS-SKII-3XHA-fSNAP-Phleomycin

   Plasmid

   #188915

   Purpose

   Integration cassette to create C-terminal fusions for fluorescent tagging

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   3XHA-fSNAP-Phleomycin
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDZ276 (pURA MET28 PCP-2x-yeGFP)

    Plasmid

    #35194

    Depositor

    Robert Singer

    Article

    Larson et al Science. 2011 Apr 22;332(6028):475-8.

    Insert

    PCP-2x-yeGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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