8,058 results

1. ZJOM100

   Plasmid

   #133662

   Purpose

   Integration of MNN9-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast early golgi.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-MNN9-mTagBFP2 (MNN9 Budding Yeast)
   

   Use

   Tags

   mTagBFP2

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS426-GFP-Osh1-PH

   Plasmid

   #71372

   Purpose

   For analysis of PH domain localization in yeast

   Depositor

   Mark Lemmon

   Article

   Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

   Insert

   Osh1-PH
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Pleckstrin homology (PH) domain; aa267-388

   Promoter

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pAV0478

   Plasmid

   #133498

   Purpose

   Expression of sfGFP targeted to the nucleus with two NLS signals

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pHis5(StuI)-p(tdh1)-NLSSV40-sfGFP-NLSSV40-natMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAG413 GAL ORF1 Shuffle1 NLS

   Plasmid

   #145794

   Purpose

   mPing Yeast Transposition Assay

   Depositor

   Charles Nathan Hancock

   Article

   Payero et al Journal of the South Carolina Academy of

   Insert

   ORF1 Shuffle1 NLS
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Enhanced Nucleur Localization Signal

   Promoter

   Available since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG425 GAL Pong TPase L418A, L420A

   Plasmid

   #145795

   Purpose

   mPing Yeast Transposition Assay

   Depositor

   Susan Wessler, Charles Nathan Hancock

   Article

   Hancock et al Mob DNA. 2010 Feb 1;1(1):5. doi: 10.1186

   Insert

   Pong TPase L418A, L420A
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   L418A, L420A

   Promoter

   Available since

   Jan. 20, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. p415 Glo3

   Plasmid

   #112651

   Purpose

   Expression S.cerevisiae

   Depositor

   Blanche Schwappach

   Article

   Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

   Insert

   Glo3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Met25

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG425GAL-ccdB-HA

   Plasmid

   #14249

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   3HA

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJL-mGold

   Plasmid

   #157997

   Purpose

   Expression of mGold in yeast cells

   Depositor

   Francois St-Pierre

   Article

   Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

   Insert

   mGold
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   mGold is mVenus with L46F;T63S mutations

   Promoter

   pTDH(GAP promoter)

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pICXC3GH

   Plasmid

   #49023

   Purpose

   FX cloning Pichia pastoris expression vector with AOX1 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tag

   Depositor

   Eric Geertsma, Raimund Dutzler

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Use

   Tags

   3C protease site (PreScission site), GFP, and dec…

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCfB4666

    Plasmid

    #106144

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFA6a-link-yoGFPgamma-SpHis5

    Plasmid

    #44859

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized GFPgamma with SpHis5 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoGFPgamma
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pG23A

    Plasmid

    #102884

    Purpose

    Plasmid used for the C-terminal tagging of Cdc14 with mCherry and the auxin-inducible degron in yeast.

    Depositor

    Matthias Heinemann

    Article

    Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

    Insert

    Cdc14/mCherry-AID(71-114)-NatMX (CDC14 Budding Yeast)
    

    Use

    Tags

    AID(71-114) and mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Native promoter

    Available since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. 5538 pUG2-SSA1

    Plasmid

    #1247

    Depositor

    Susan Lindquist

    Article

    Glover et al Cell 1998 Jul 10;94(1):73-82.

    Insert

    Ssa1 (SSA1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pESC-URA-Rnq1-GFP

    Plasmid

    #21051

    Depositor

    Judith Frydman

    Article

    Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

    Insert

    Rnq1 (RNQ1 Budding Yeast)
    

    Use

    Yeast/bacteria shuttle

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pBBK67 pHomL-RPL18Bp-RPC19-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

    Plasmid

    #179051

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of RPC19-Link-Scarlet(rfp), a marker for the nucleolus

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-RPC19-Link-Scarlet(rfp)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUC19-URA3-3'SEC13-GFP

    Plasmid

    #25445

    Depositor

    Benjamin Glick

    Article

    Rossanese et al J Cell Biol. 1999 Apr 5. 145(1):69-81.

    Insert

    Sec13-Green Fluorescent Protein
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSM1960

    Plasmid

    #41842

    Depositor

    Susan Michaelis

    Article

    Metzger et al J Biol Chem. 2008 Nov 21;283(47):32302-1

    Insert

    SEC63-mRFP
    

    Use

    Tags

    mRFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p42Hyg 1-F GW

    Plasmid

    #58551

    Purpose

    Multicopy vector with Hygromycin resistance marker for single Gateway insertion

    Depositor

    Leonid Kruglyak

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pM

    Plasmid

    #64179

    Purpose

    harbours marker gene MET17

    Depositor

    Markus Ralser

    Article

    Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Aug. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRG710_lexO-HO_LexA-TF_HIS3MX

    Plasmid

    #154819

    Purpose

    Inducible expression of HO endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    HO endonuclease (HO Budding Yeast)
    LexA-ER-B112
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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