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ZJOM11

Plasmid

#133637

Purpose

Integration of GFP-TLG1 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast late golgi/early endosome.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

pCUP1-GFP-Tlg1 (TLG1 Budding Yeast)

Tags

EGFP

Expression

Yeast

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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ZJOM156

Plasmid

#133636

Purpose

Integration of 2xGFP tag at SEC7 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast late golgi/early endosome.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

SEC7-2GFP (SEC7 Budding Yeast)

Tags

EGFP

Expression

Yeast

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pMEL10

Plasmid

#107916

Purpose

URA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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Cip-pAct1-Mnase-SAT1

Plasmid

#162557

Purpose

For the C. auris MNase control strain, the URA3 auxotrophy marker of the CIp-pACT1-3xFLAG-MNase-SV40-CYC was replaced by SAT1

Depositor

Adnane Sellam

Article

Tebbji et al mSphere. 2020 Oct 14;5(5). pii: 5/5/e006

Insert

3FLAG-Mnase-SV40

Expression

Yeast

Promoter

Actine

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pJW1666

Plasmid

#112040

Purpose

CEN/ARS plasmid for GAL-inducible empty reporter V5-TEV-EGFP tagged expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

None

Tags

V5-TEV-EGFP

Expression

Yeast

Promoter

GAL1

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pGFP-ATG13(315)

Plasmid

#122072

Purpose

Expressing GFP-Atg13

Depositor

Daniel Klionsky

Article

Chew et al Autophagy. 2015;11(6):891-905. doi: 10.1

Insert

ATG13

Tags

GFP

Expression

Yeast

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pSSN6

Plasmid

#104340

Purpose

Express SSN6

Depositor

Alexander Varshavsky

Article

Xia et al J Biol Chem. 2008 Oct 24;283(43):28958-6

Insert

SSN6 (CYC8 Budding Yeast)

Expression

Yeast

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pKG-GW1

Plasmid

#203163

Purpose

Yeast expression vector; To express proteins under control of TDH3 promoter and terminator using leucine selection

Depositor

William Sessa

Article

Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pVG47

Plasmid

#164705

Purpose

yEVenus under the control of tetOpr

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

Insert

tetOpr - yEVenus - CLN2 PEST - ADH1t

Expression

Yeast

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pJZC603

Plasmid

#62317

Purpose

sgRNA with 2x PP7 for yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA + 2x PP7 RNA binding module

Expression

Yeast

Promoter

SNR52

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pGWKS10

Plasmid

#139417

Purpose

Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Clover marker.

Depositor

James Fraser

Article

Spencer et al Fungal Genet Biol. 2020 May;138:103365.

Insert

Clover

Expression

Yeast

Promoter

TEF1

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1517

Plasmid

#62387

Purpose

pFA6a Osm1(M33A)-yoEGFP::LEU2

Depositor

Jonathan Weissman

Article

Williams et al Science. 2014 Nov 7;346(6210):748-51. do

Insert

OSM1-EGFP fusion

Tags

EGFP

Expression

Yeast

Mutation

Methionine 33 mutated to Alanine

Promoter

Adh1p

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pAH243

Plasmid

#121147

Purpose

Expression of both nmt41p-cas9 and rrk1p-gRNA (ura4 marker) for CRISPR genome editing in fission yeast

Depositor

Katsunori Tanaka

Article

Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

Inserts

humanized Streptococcus pyogenes Cas9
gRNA expression module

Use

CRISPR

Tags

Flag

Expression

Yeast

Promoter

S.pombe rrk1 and nmt41

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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TetO7-HSP30UTR-LacZ-PKTlinker-nLucPEST-MS2(v4)

Plasmid

#199763

Purpose

Elongation reporter construct to quantify elongation of LacZ

Depositor

Brian Zid

Article

Guzikowski et al RNA Biol. 2022;19(1):636-649. doi: 10.10

Insert

LacZ-nLucPEST

Expression

Yeast

Promoter

TetO7

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pRS406-CMV-lacI-3FLAG

Plasmid

#83410

Purpose

Expression of lacI in yeast

Depositor

Toshi Tsukiyama

Article

Unnikrishnan et al Nat Struct Mol Biol. 2010 Apr;17(4):430-

Insert

lacI repressor

Tags

3xFLAG and NLS

Expression

Yeast

Promoter

CMV

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pRS303 Gal TDP-43 YFP

Plasmid

#27468

Depositor

Aaron Gitler

Article

Johnson et al Proc Natl Acad Sci U S A. 2008 Apr 29. 1

Insert

TDP-43 (TARDBP Human)

Tags

YFP

Expression

Yeast

Mutation

D23A mutation in TDP-43 sequence

Available Since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only
pAV0751

Plasmid

#133485

Purpose

Expression in fission yeast from promoter pnmt1 , integrated at ura4 locus

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(nmt1)-sfGFP-terminator(ScCYC1)

Expression

Yeast

Available Since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pJZC620

Plasmid

#62282

Purpose

Expresses dCas9, MCP-VP64, and PCP-VP64 in Yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

MCP-VP64
PCP-VP64
dCas9

Tags

VP64

Expression

Yeast

Mutation

Nuclease activity has been inactivated by mutatio…

Promoter

pAdh and pTdh3

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-natMX6-P3nmt1-3FLAG

Plasmid

#19345

Depositor

Eishi Noguchi

Article

Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

Insert

natMX6

Expression

Yeast

Available Since

Sept. 25, 2008

Availability

Academic Institutions and Nonprofits only

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pRS315-ADH700p-yeCherry-p150-yeGFP-CYCt

Plasmid

#194519

Purpose

Dual fluorescence reporter for studying protein degradation

Depositor

Tommer Ravid

Article

Mashahreh et al Nat Commun. 2022 Dec 8;13(1):7588. doi:

Insert

TIF4631 leader sequence

Expression

Yeast

Available Since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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