8,350 results
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p416TEF1 Cystector (Cysteine biosensor)
Plasmid#250654PurposeCytosolic expression plasmid for Cystector (Cysteine biosensor) in Saccharomyces cerevisiaeDepositorInsertCystector (Cysteine biosensor)
ExpressionYeastPromoterTEF1Available SinceMarch 24, 2026AvailabilityAcademic Institutions and Nonprofits only -
MTS-ABE8e-nCas9-V5-P2A-EGFP
Plasmid#251964PurposeExpression of ABE8e (V106W with spCas9 D10A) in mitochondria for yeast mtDNA editingDepositorInsertABE8e
UseCRISPRTagsMTS and V5-P2A-EGFPExpressionYeastPromoterGPDAvailable SinceMarch 18, 2026AvailabilityAcademic Institutions and Nonprofits only -
MTS-ABE8e-Cas12
Plasmid#251966PurposeExpression of ABE8e (with LbCpf1) in mitochondria for yeast mtDNA editingDepositorInsertABE8e-LbCpf1
UseCRISPRTagsMTS and V5-P2A-EGFPExpressionYeastPromoterGPDAvailable SinceMarch 18, 2026AvailabilityAcademic Institutions and Nonprofits only -
sgRNA-IM83
Plasmid#251967PurposesgRNA with IM83 aptamer for yeast mtDNA editingDepositorInsertsgRNA-IM83
UseCRISPRExpressionYeastPromoterSNR52Available SinceMarch 18, 2026AvailabilityAcademic Institutions and Nonprofits only -
sgRNA-msIM83
Plasmid#251968PurposesgRNA for yeast mtDNA editing, with IM83 aptamer inserted into the major stem-loop of the sgRNADepositorInsertsgRNA-msIM83
UseCRISPRExpressionYeastPromoterSNR52Available SinceMarch 18, 2026AvailabilityAcademic Institutions and Nonprofits only -
pYO950
Plasmid#235722PurposeExpression of FL yeast VPS15-3x FLAG under its own promoter in yeastDepositorArticleInsertVPS15
Tags3xFLAGExpressionYeastPromoterScVPS15 promoterAvailable SinceMarch 18, 2026AvailabilityAcademic Institutions and Nonprofits only -
pYO1877
Plasmid#235723PurposeExpression of FL yeast VPS15 S622D_H623D_T626E mutant-3x FLAG under its own promoter in yeastDepositorArticleInsertVPS15
Tags3xFLAGExpressionYeastMutationS622D, H623D, T626EPromoterScVPS15 promoterAvailable SinceMarch 18, 2026AvailabilityAcademic Institutions and Nonprofits only -
pYO1922
Plasmid#235768PurposeProtein expression of ScVPS38+ScVPS30-mCherryDepositorArticleTagsmCherryExpressionYeastPromoterGAL-TDH3Available SinceMarch 18, 2026AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mIAA7-mCherry-klTRP1
Plasmid#247623PurposeYeast gene tagging with mIAA7DepositorInsertmIAA7
TagsmCherryExpressionYeastAvailable SinceMarch 18, 2026AvailabilityAcademic Institutions and Nonprofits only -
pRS316 Gal1p1
Plasmid#242951PurposeBackbone plasmid for subcloningDepositorInsertGal_promoter-cycE terminator
TagsMyc-tagExpressionYeastAvailable SinceMarch 17, 2026AvailabilityAcademic Institutions and Nonprofits only -
pRS316 Gal1p1 IpgA
Plasmid#242952PurposeExpression of IpgA in yeast cellsDepositorInsertIpgA (IcsB Chaperone)
TagsMycExpressionYeastAvailable SinceMarch 17, 2026AvailabilityAcademic Institutions and Nonprofits only -
pRS313 Gal1p2
Plasmid#242948PurposeBackbone plasmid for IcsB and homologs subcloningDepositorInsertBackbone plasmid for subcloning
Tags3X FLAG tagExpressionYeastAvailable SinceMarch 17, 2026AvailabilityAcademic Institutions and Nonprofits only -
pRS313 Gal1p2 IcsB
Plasmid#242949PurposeExpression of IcsB in yeastDepositorInsertIcsB
Tags3X FLAG tagExpressionYeastAvailable SinceMarch 17, 2026AvailabilityAcademic Institutions and Nonprofits only -
pRS313 Gal1p2 H8
Plasmid#242950PurposeExpression of homolog H8 in yest cellsDepositorInsertIcsB Homolog H8
Tags3X FLAG tagExpressionYeastAvailable SinceMarch 17, 2026AvailabilityAcademic Institutions and Nonprofits only -
pRS316-pTEF-Sc_IntAct-tCYC
Plasmid#248980PurposeLow-copy centromeric plasmids which expresses Saccharomyces cerevisiae IntAct (actin internally tagged with ALFA tag between T229 and A230 amino acids)DepositorInsertSc_IntAct
TagsALFA tagExpressionYeastPromoterTEFAvailable SinceMarch 11, 2026AvailabilityAcademic Institutions and Nonprofits only -
pDUAL-pAct1-Sp_IntAct-tADH1
Plasmid#250862PurposeLow-copy centromeric plasmids which expresses Schizosaccharomyces pombe IntAct (actin internally tagged with ALFA tag between T229 and A230 amino acids)DepositorInsertSp_IntAct
TagsALFA tagExpressionYeastPromoterTEFAvailable SinceMarch 11, 2026AvailabilityAcademic Institutions and Nonprofits only -
pPT24-mtArg8m*-V5-UTR-HH-sgRNA_ATP6-HDV
Plasmid#251981PurposeFor biolistic transformation to express Arg8m* and control sgRNA targeting ATP6 from mtDNADepositorInsertsmtArg8m*
HH-sgRNA_ATP6-HDV
TagsV5ExpressionYeastMutationpremature stop CAA->TAA, Q79*Available SinceMarch 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
pVG173
Plasmid#251208PurposeExpresses a version of light-inducible transcription factor EL222 (Gly80Arg) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
UseSynthetic BiologyExpressionBacterial and YeastMutationGly80ArgPromoterPGK1prAvailable SinceMarch 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
pVG298
Plasmid#227762PurposeExpresses a version of light-inducible transcription factor EL222 (Thr83Ala) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
ExpressionYeastMutationThr83Ala mutantPromoterPGK1prAvailable SinceMarch 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
pADH1-MTS-ABE8e-P2A-EGFP
Plasmid#251973PurposeYeast mtDNA editing with a mid-strength promoter for lower expression levelDepositorInsertABE8e
UseCRISPRTagsMTS and V5-P2A-EGFPExpressionYeastPromoterADH1Available SinceFeb. 24, 2026AvailabilityAcademic Institutions and Nonprofits only -
pPT24-mtArg8m-V5
Plasmid#251979PurposeFor biolistic transformation to express Arg8m from mtDNADepositorInsertmtArg8m
TagsV5ExpressionYeastAvailable SinceFeb. 24, 2026AvailabilityAcademic Institutions and Nonprofits only -
MTS-ABE-ARG8
Plasmid#251976PurposeExpression of ABE-ARG8 fusion protein in yeastDepositorInsertABE-ARG8
UseCRISPRTagsMTSExpressionYeastPromoterGPDAvailable SinceFeb. 24, 2026AvailabilityAcademic Institutions and Nonprofits only -
NLS-ABE8e-GFP
Plasmid#251972PurposeYeast nDNA editing with ABE8eDepositorInsertABE8e
UseCRISPRTags3xNLS-3xFLAG-EGFP and NLSExpressionYeastPromoterGPDAvailable SinceFeb. 23, 2026AvailabilityAcademic Institutions and Nonprofits only -
pPT24-mtArg8m*-V5
Plasmid#251978PurposeFor biolistic transformation to express Arg8m* from mtDNADepositorInsertmtArg8m*
TagsV5ExpressionYeastMutationpremature stop CAA->TAA, Q79*Available SinceFeb. 23, 2026AvailabilityAcademic Institutions and Nonprofits only -
pRS426-SNR52-crRNA
Plasmid#251984PurposeExpression of crRNA (version 3) for editing, used together with tracrRNA Plasmid #251985. This plasmid is also called crRNA in the paper.DepositorInsertcrRNA
UseCRISPRExpressionYeastPromoterSNR52Available SinceFeb. 23, 2026AvailabilityAcademic Institutions and Nonprofits only -
pPT24-mtArg8m*-V5-UTR-HH-ntsgRNA-HDV
Plasmid#251982PurposeFor biolistic transformation to express Arg8m* and a non-targeting control sgRNA from mtDNADepositorInsertsmtArgm*
HH-nt sgRNA-HDV
TagsV5ExpressionYeastMutationpremature stop CAA->TAA, Q79*Available SinceFeb. 23, 2026AvailabilityAcademic Institutions and Nonprofits only -
nArg8-EGFP
Plasmid#251977PurposeExpression of nuclear ARG8-EGFP as controlDepositorInsertnArg8-EGFP
TagsEGFPExpressionYeastPromoterGPDAvailable SinceFeb. 23, 2026AvailabilityAcademic Institutions and Nonprofits only -
pRembiv
Plasmid#252216PurposeYeast expression of BK polyomavirus VP1 and aeBlue reporterDepositorInsertsBKV-IV VP1
aeBlue
Formaldehyde dehydrogenase 1
ExpressionYeastPromoterFDH1 and MAL32Available SinceFeb. 23, 2026AvailabilityIndustry, Academic Institutions, and Nonprofits -
HRPEadh-pAG303
Plasmid#249735PurposeDerived from pAG303GPD-ccdb (#14134); GPD replaced with a synthetic 5xHRPE-ADHutr sequence for orthogonal activation by plant RAP2.12-based TFs.DepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 9, 2026AvailabilityAcademic Institutions and Nonprofits only -
SYRAP_pDONR201
Plasmid#249814PurposeEncodes SYRAP (RAP2.12-GAL4STE12), Gateway compatible as Donor plasmidDepositorInsertRAP2.12-GAL4STE (RAP2.12 Budding Yeast, Mustard Weed)
UseSynthetic BiologyTagsGAL4-STE12ExpressionPlant and YeastAvailable SinceFeb. 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
HRPEadh-pAG305
Plasmid#249734PurposeDerived from pAG305GPD-ccdb (#14138); GPD replaced with a synthetic 5xHRPE-ADHutr sequence for orthogonal activation by plant RAP2.12-based TFs.DepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
PCUP1-GFP-PHO8 K(24,25,30,33)R
Plasmid#207044PurposeExpression of GFP-Pho8 point mutant under CUP1 promoter.DepositorInsertPHO8
TagsGFPExpressionYeastMutationK(24,25,30,33)RPromoterpCUP1Available SinceFeb. 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mIAA7-mCherry-kanNP1
Plasmid#247621PurposeYeast gene tagging with mIAA7DepositorInsertmIAA7
TagsmCherryExpressionYeastAvailable SinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
pSC6
Plasmid#233020PurposeInducible Conjugative PlasmidDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
pAG304-ccdbNLUC
Plasmid#249736PurposeDerived from pAG304GPD-ccdb (#14136); GPD removed and NLUC cds inserted downstream gateway cassette. Useful for cloning promoter-NLUC fusions in Saccharomyces cerevisiaeDepositorTypeEmpty backboneExpressionYeastAvailable SinceJan. 28, 2026AvailabilityAcademic Institutions and Nonprofits only -
UbSYRAP_pDONR201
Plasmid#249816PurposeEncodes UbSYRAP, a SYRAP split version (UBQ-RAP2.12-GAL4STE12), Gateway compatible as Donor plasmidDepositorInsertUBQ-RAP2.12-GAL4STE (RAP2.12 Budding Yeast, Mustard Weed)
UseSynthetic BiologyTagsGAL4-STE12 and UBQExpressionPlant and YeastAvailable SinceJan. 28, 2026AvailabilityAcademic Institutions and Nonprofits only -
ClhN-p1k-Sec7-4V5-APEX2-URA
Plasmid#207060PurposeExpressing Sec7-4V5-APEX2. Insufficient signal for EM.DepositorInsertSec7
Tags4xV5-APEX2ExpressionYeastPromoterpSEC7Available SinceJan. 27, 2026AvailabilityAcademic Institutions and Nonprofits only -
P1K-3HA-Atg8-MET15
Plasmid#207032PurposeExpression of 3HA-Atg8.DepositorInsertATG8
Tags3xHAExpressionYeastPromoterpATG8Available SinceJan. 27, 2026AvailabilityAcademic Institutions and Nonprofits only -
ClhN-P1K-3VSV-Atg8(T56A T87A)-MET15
Plasmid#207048PurposeExpression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).DepositorInsertATG8
Tags3xVSVExpressionYeastMutationT56A T87AAvailable SinceJan. 27, 2026AvailabilityAcademic Institutions and Nonprofits only -
ClhN-P1K-3VSV-Atg8(P85A P86A)-MET15
Plasmid#207049PurposeExpression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).DepositorInsertATG8
Tags3xVSVExpressionYeastMutationP85A P86AAvailable SinceJan. 27, 2026AvailabilityAcademic Institutions and Nonprofits only -
ClhN-p1k-Snf7-4V5-APEX2-URA
Plasmid#207058PurposeExpressing Snf7-4V5-APEX2. Insufficient signal for EM.DepositorInsertSnf7
Tags4xV5-APEX2ExpressionYeastPromoterpSNF7Available SinceJan. 27, 2026AvailabilityAcademic Institutions and Nonprofits only -
pAvA143
Plasmid#248147Purposemutated LuxR transcriptional activator for expression in yeast: fused with Gal4 activation domain with 'gen2' mutationsDepositorInsertluxR (gen3 mutations)
UseIntegration vectorTagsGal4 activation domain and nuclear localization s…ExpressionYeastMutationGal4_AD: F51 (TTC→TTT), G88E. LuxR: K53E, N86Y, I…PromoterpPGK1Available SinceJan. 15, 2026AvailabilityAcademic Institutions and Nonprofits only -
pAvA141
Plasmid#248146Purposemutated LuxR transcriptional activator for expression in yeast: fused with Gal4 activation domain with 'gen2' mutationsDepositorInsertluxR (gen2 mutations)
UseIntegration vectorTagsGal4 activation domain and nuclear localization s…ExpressionYeastMutationGal4_AD: G8V. NLS: K120N. LuxR: N86K, K104E, M135VPromoterpPGK1Available SinceJan. 15, 2026AvailabilityAcademic Institutions and Nonprofits only -
pAvA139
Plasmid#248145Purposemutated LuxR transcriptional activator for expression in yeast: fused with Gal4 activation domain with 'gen1' mutationsDepositorInsertluxR (gen1 mutations)
UseIntegration vectorTagsGal4 activation domain and nuclear localization s…ExpressionYeastMutationGal4_AD: N24K, P41 (CCA→CCG), N46D, T92S, V98 (GT…PromoterpPGK1Available SinceJan. 15, 2026AvailabilityAcademic Institutions and Nonprofits only -
P2802
Plasmid#248828PurposeAll repressors for the WTC847 system.DepositorInsertLexA-L7-hER(L387M), LexA-hER-adh1tail
ExpressionYeastMutationLexA-L7-hER: L387MPromoterpACT1 (LexA-L7-hER(L387M)), P7SulA.1(LexA-hER-adh…Available SinceJan. 15, 2026AvailabilityAcademic Institutions and Nonprofits only -
P2814
Plasmid#248829PurposeAll repressors for the WTC848 system.DepositorInsertLacI(6m), LacI(5m)
TagsSV40NLS on LacI(6m)ExpressionYeastMutationLacI(6m): T167A, W220F, M254L, V119I, Q60G, M242L…PromoterpRnr2 (LacI(6m), P4Lacn.2 (LacI(5m))Available SinceJan. 15, 2026AvailabilityAcademic Institutions and Nonprofits only -
P2880
Plasmid#248830PurposeAll repressors for the WTC848 system.DepositorInsertLacI(6m), LacI(5m)
TagsSV40NLS on LacI(6m)ExpressionYeastMutationLacI(6m): T167A, W220F, M254L, V119I, Q60G, M242L…PromoterpRnr2 (LacI(6m), P4Lacn.2 (LacI(5m))Available SinceJan. 15, 2026AvailabilityAcademic Institutions and Nonprofits only -
P2881
Plasmid#248831PurposeAll repressors for the WTC848 system.DepositorInsertLacI(6m), LacI(5m)
TagsSV40NLS on LacI(6m)ExpressionYeastMutationLacI(6m): T167A, W220F, M254L, V119I, Q60G, M242L…PromoterpRnr2 (LacI(6m), P4Lacn.2 (LacI(5m))Available SinceJan. 15, 2026AvailabilityAcademic Institutions and Nonprofits only