8,173 results
-
Plasmid#162041PurposeExpresses Cas9 and contains kanR468 guide which targets the kanR geneDepositorInsertkanR468 guide
UseCRISPRExpressionYeastAvailable SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
p414GPD-3xFLAG-GW
Plasmid#63780Purposeyeast expression vector p414GPD modified such that it is Gateway converted and introduces a 3xFLAG tag at the N-terminus of the insertDepositorTypeEmpty backboneTags3xFLAGExpressionYeastPromoterGPDAvailable SinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG416-GAL-FUS
Plasmid#181711PurposeGalactose inducible expression of FUSDepositorInsertFUS (FUS Human)
ExpressionYeastAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDRF1 mStayGold-T2A-mCherry
Plasmid#219834Purposeequimolar expression of mStaygold and mCherry in budding yeastDepositorInsertmStayGold-T2A-mCherry
ExpressionYeastAvailable SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV0478
Plasmid#133498PurposeExpression of sfGFP targeted to the nucleus with two NLS signalsDepositorInsertpHis5(StuI)-p(tdh1)-NLSSV40-sfGFP-NLSSV40-natMX
ExpressionYeastAvailable SinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP2_leu2d_NRT1.1
Plasmid#58332PurposeExpresses Arabidopsis thaliana NRT1.1 in Saccharomyces cells as a C-terminal GFP his tagged proteinDepositorAvailable SinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046233
Plasmid#149032PurposeYeast Expression plasmid for SARS-CoV-2 nsp3DepositorInsertSARS-CoV-2 nsp3 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
TagsCleavable thrombin;6xHISExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpTEF1Available SinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJJH1260
Plasmid#36913DepositorInsertsScURA3
ScURA3
ExpressionBacterial, Mammalian, and Y…Available SinceJuly 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
YIp ADE2-HphMx
Plasmid#122916PurposeADE2-HphMx integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailable SinceOct. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEM09
Plasmid#135479PurposeSpizellomyces Hsp70 promoter driving expression of Hygromycin resistance fused to GFPDepositorInsertSpunHsp70pr
TagsGFP and hphExpressionBacterial and YeastPromoterSpun Hsp70prAvailable SinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G4-Ph
Plasmid#46820PurposeTEF1 and HXT7 promoter controlled expression cassettes with phleomycin resistance for yeast transformationDepositorTypeEmpty backboneTagsFLAG and c-mycExpressionYeastPromoterTEF1 and HXT7Available SinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
LPH2782 - Ub-K48R
Plasmid#32161DepositorExpressionYeastMutationK48RPromoterCUP1Available SinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pWS3868
Plasmid#185745PurposeSub-array backbone 4/4DepositorTypeEmpty backboneExpressionYeastAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYC34
Plasmid#63915PurposeRecipient overexpressing vector to construct mCherry tagged fusionsDepositorTypeEmpty backboneTagsmCherryExpressionYeastPromoterTEF1 (Saccharomyces cerevisiae)Available SinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pINER5
Plasmid#185895PurposeIn vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).DepositorInsertPRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PSk.GAL2>YFAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2
ExpressionYeastMutationNoneAvailable SinceAug. 25, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDS1885
Plasmid#177341PurposeExpresses Aequorin in Candida albicans using the dominant marker SAT1DepositorInsertAequorin
ExpressionYeastPromoterACT1Available SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pG23A
Plasmid#102884PurposePlasmid used for the C-terminal tagging of Cdc14 with mCherry and the auxin-inducible degron in yeast.DepositorInsertCdc14/mCherry-AID(71-114)-NatMX (CDC14 Budding Yeast)
TagsAID(71-114) and mCherryExpressionYeastPromoterNative promoterAvailable SinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046483
Plasmid#153868PurposeYeast expression plasmid for SARS-CoV-2 nsp3DepositorInsertSARS-CoV-2 nsp3 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
TagsCleavable thrombin;6xHISExpressionYeastMutationwtPromoterpGAL1Available SinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPTK005-3a-αMF
Plasmid#84964PurposeType 3a, α-mating factor - secretion signalDepositorInsertα-mating factor secretion tag
UseSynthetic BiologyExpressionYeastPromoterNonAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCfB4666
Plasmid#106144PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistanceDepositorTypeEmpty backboneExpressionYeastAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only