8,418 results
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Plasmid#107931PurposeTRP1 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRExpressionYeastAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pROS16
Plasmid#107930PurposeHIS3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRExpressionYeastAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pROS11
Plasmid#107925PurposeamdS based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRExpressionYeastAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMEL17
Plasmid#107923PurposeTRP1 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y
UseCRISPRExpressionYeastAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
POM42-mTFP1
Plasmid#69852PurposeN-terminal tagging with mTFP1 using URA3 as a selection marker in S. cerevisiae.DepositorInsertmTFP1 minus start and stop codon
ExpressionYeastAvailable sinceApril 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYZ165
Plasmid#98418PurposePombe Expression Vector - nmt1 promoter with Sp.his3 markerDepositorTypeEmpty backboneExpressionYeastAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXNgate21-3HA
Plasmid#105078PurposeYeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloningDepositorTypeEmpty backboneTagsNubGExpressionYeastAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-VPS8-iGFPx6
Plasmid#105271PurposePlasmid for pop-in/pop-out replacement of Vps8 with Vps8-iGFPx6.DepositorAvailable sinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHES875
Plasmid#89192PurposeExpresses mCherry under control of GAL1 promoter.DepositorInsertpGAL1-mCherry
ExpressionYeastPromoterGAL1Available sinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-iGFP-TLG2
Plasmid#105262PurposePlasmid for pop-in/pop-out replacement of Tlg2 with iGFP-Tlg2.DepositorAvailable sinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUDP044
Plasmid#101168PurposepUDP004 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes SeATF1 and SeATF2 and Spcas9D147Y P411T in S. pastorianus (HH-gRNASeATF1-HDV-linker-HH-gRNASeATF2-HDV)DepositorInsertpolycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus
UseCRISPRExpressionYeastPromoterScTDH3Available sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUDP003
Plasmid#101164PurposeE. coli/S. cerevisiae amdS shuttle vector allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)DepositorTypeEmpty backboneUseCRISPRExpressionYeastPromoterScTDH3Available sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUDE722
Plasmid#103022Purposeexpression of a Cpf1 programming crRNA targetting CAN1 (crCAN1-4S)DepositorAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHES873
Plasmid#87945PurposeExpresses estradiol-inducible synthetic transcription factor in yeast under control of GAL1 promoter. It activates transcription of promoters containing Zif268 binding sites.DepositorInsertpGAL1- Zif268 DBD - hPR LBD - MSN2 AD (PGR Human, Budding Yeast)
ExpressionYeastPromoterGAL1Available sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
p416TEF-SEC16
Plasmid#102676PurposeThe gene SEC16 is driven by the strong promoter TEF. Overexpression of SEC16 increases recombinant protein secretionDepositorAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCTcon2-5INS_EV3
Plasmid#102293PurposeYeast Surface Display of 5Ins_16_EV3DepositorInsertEvolved Insulin binding scFV
Tags3x(G4S) Linker, G3S Linker, HA, and MycExpressionYeastPromoterGAL1Available sinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDR FLIP43 GPS1-NR
Plasmid#101085PurposeGA biosensor expression plasmidDepositorInsertGPS1-NR
Tags6HIS and cMycExpressionYeastMutationAtGAI E51K E54R, AtGID1C S114A F115APromoterpPMA1Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
YIp211-P30-His-POL30-3'
Plasmid#99546PurposeYeast integrative vector for expression of His-tagged POL30 (PCNA); URA3 markerDepositorAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR221-Sen1
Plasmid#99994PurposeShuttle yeast Sen1 into Gateway destination vectorDepositorInsertSen1 (SEN1 Budding Yeast)
ExpressionYeastAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJK148-Pnmt41-GBP-mCherry(C)-leu1+
Plasmid#89069Purposegenomic GBP-mCherry epitope C-terminal tagging at leu1+ locus in S. pombeDepositorTypeEmpty backboneUseGenomic integration in s. pombeTagsGBP-mCherryExpressionYeastAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only