8,350 results

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pRO112

Plasmid

#233806

Purpose

MoClo-YTK; type3; rtTA systems; rtTA-v10

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

rtTA-v10

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRO217

Plasmid

#233807

Purpose

MoClo-YTK; type3; rtTA systems; rtTA-v16

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

rtTA-v16

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRO232

Plasmid

#233808

Purpose

MoClo-YTK; type2A; ABA systems; phlfO7

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

phlfO7

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRO175

Plasmid

#233810

Purpose

MoClo-YTK; type3B; ABA systems; ABI

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

ABI

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRO177

Plasmid

#233811

Purpose

MoClo-YTK; type3B; ABA systems; PYL1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

PYL1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRO286

Plasmid

#233812

Purpose

MoClo-YTK; type2A; Auxin systems; amtRO7

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

amtRO7

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRO271

Plasmid

#233813

Purpose

MoClo-YTK; type3A; Auxin systems; AmtR-NES

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

AmtR-NES

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pWN022

Plasmid

#233814

Purpose

MoClo-YTK; type3B; Auxin systems; AIDΔ34

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

AIDΔ34

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pWN050

Plasmid

#233815

Purpose

MoClo-YTK; type3B; Auxin systems; TIR1-U1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

TIR1-U1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pWN083

Plasmid

#233816

Purpose

MoClo-YTK; type3B; Auxin systems; TIR1-U2

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

TIR1-U2

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAL009

Plasmid

#233817

Purpose

MoClo-YTK; type3B; NS3 systems; NS3-V1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

NS3-V1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pWN018

Plasmid

#233818

Purpose

MoClo-YTK; type3B; NS3 systems; NS3-V2

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

NS3-V2

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pWN079

Plasmid

#233819

Purpose

MoClo-YTK; type3B; NS3 systems; NS3a

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

NS3a

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pWN080

Plasmid

#233820

Purpose

MoClo-YTK; type3B; NS3 systems; Linker-NS3a

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

Linker-NS3a

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pWN043

Plasmid

#233821

Purpose

MoClo-YTK; type3B; NS3 systems; DNCR2

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

DNCR2

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pWN062

Plasmid

#233822

Purpose

MoClo-YTK; type3A; NS3 systems; LexA-NES

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

LexA-NES

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRO176

Plasmid

#233824

Purpose

MoClo-YTK; type3A; Activation domains; NLS-VP48

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

NLS-VP48

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO411

Plasmid

#235732

Purpose

Protein expression of ZZ-FL yeast Atg14 in yeast

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

ATG14

Tags

3xTEV-ZZ

Expression

Yeast

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO379

Plasmid

#235733

Purpose

Protein expression of VPS34 + VPS15-ZZ + ZZ-VPS30 (all yeast) in yeast

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS34 (VPS34 Budding Yeast)
VPS15 (VPS15 Budding Yeast)
VPS30 (VPS30 Budding Yeast)

Tags

3xTEV-ZZ and ZZ-3xTEV

Expression

Yeast

Mutation

S2A on ScVPS34

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO359

Plasmid

#235750

Purpose

Protein expression of FL ScVPS34 and FL ScVPS15-ZZ

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS34 (VPS34 Budding Yeast)
VPS15 (VPS15 Budding Yeast)

Tags

3xTEV-ZZ

Expression

Yeast

Mutation

S2A, aa 3-887 and T134A, I851R

Promoter

GAL-TDH3

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO734

Plasmid

#235753

Purpose

Protein expression of ScVPS30 and ZZ-ScVPS38

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS30 (VPS30 Budding Yeast)
VPS38 (VPS38 Budding Yeast)

Tags

ZZ-3xTEV

Expression

Yeast

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAPInt-mNeonGreen-G418

Plasmid

#236484

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mNeonGreen and selection on G418.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

mNeonGreen

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO831

Plasmid

#235754

Purpose

Protein expression of ZZ-ScVPS38 (1-212F)

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS38

Tags

ZZ-3xTEV

Expression

Yeast

Mutation

aa 1-212F

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO934

Plasmid

#235766

Purpose

Expression of ScVPS34 delta CBR1 L31-D55-3xFLAG undeer its own promoter

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34 delta CBR1

Tags

3xFLAG

Expression

Yeast

Mutation

aa 31-55 deleted

Promoter

ScVPS34 promoter

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO225

Plasmid

#235729

Purpose

Protin expression of FL yeast VPS15 -ZZ in yeast

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS15

Tags

3xTEV-ZZ

Expression

Yeast

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO290

Plasmid

#235731

Purpose

Protein expression in FL yeast VPS34 D731N mutant in yeast

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34

Expression

Yeast

Mutation

S2A, D731N

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO69

Plasmid

#235747

Purpose

Protein expression of FL ScVPS34 in yeast

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34

Expression

Yeast

Mutation

S2A

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO361

Plasmid

#235748

Purpose

Protein expression of ScVPS34 HELCAT

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34 HELCAT

Expression

Yeast

Mutation

aa 268-875

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO227

Plasmid

#235749

Purpose

Protein expression of ZZ-FL ScVPS30

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS30

Tags

ZZ-3xTEV

Expression

Yeast

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO832

Plasmid

#235755

Purpose

Protein expression of ScVPS30 (1-215E), untagged

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

ScVPS30

Expression

Yeast

Mutation

aa 1-215E

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO478

Plasmid

#235757

Purpose

Expression of FL ScVPS34-3xFLAG under its own promoter

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34

Tags

3xFLAG

Expression

Yeast

Promoter

ScVPS34 promoter

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO760

Plasmid

#235759

Purpose

Expression of ScVPS38 delta C2 (1-154)-x3FLAG under its own promoter

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS38 delta C2

Tags

3xFLAG

Expression

Yeast

Mutation

aa 1-154 deleted

Promoter

ScVPS38 promoter

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO761

Plasmid

#235760

Purpose

Expression of ScVPS38 delta BARA2 (320-440)-x3FLAG under its own promoter

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VSP38 delta BARA2

Tags

3xFLAG

Expression

Yeast

Mutation

aa 320-440 deleted

Promoter

ScVPS38 promoter

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO764

Plasmid

#235761

Purpose

Expression of ScVPS38-EGFP under its own promoter

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS38

Tags

EGFP

Expression

Yeast

Promoter

ScVPS38 promoter

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO765

Plasmid

#235762

Purpose

Expression of ScVPS38 delta C2 (1-154) -EGFP under its own promoter

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS38

Tags

EGFP

Expression

Yeast

Mutation

aa 1-154 deleted

Promoter

ScVPS38 promoter

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO766

Plasmid

#235763

Purpose

Expression of ScVPS38 delta BARA2 (320-440)-EGFP under its own promoter

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VSP38 delta BARA2

Tags

EGFP

Expression

Yeast

Mutation

aa 320-440 deleted

Promoter

ScVPS38 promoter

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO932

Plasmid

#235764

Purpose

Expression of ScVPS34 HELCAT (268-875)-3xFLAG under its own promoter

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34 HELCAT

Tags

3xFLAG

Expression

Yeast

Mutation

aa 268-875

Promoter

ScVPS34 promoter

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO933

Plasmid

#235765

Purpose

Expression of ScVPS34 delta C154-V202-3xFLAG under its own promoter

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34 delta C154-V202

Tags

3xFLAG

Expression

Yeast

Mutation

aa 154-202 deleted

Promoter

ScVPS34 promoter

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO503

Plasmid

#235743

Purpose

Protein expression of KmTor by integrating ZZ-KmTOR into the K. marxianus genome

Depositor

Roger Williams

Article

Baretic et al Nat Commun. 2016 Apr 13;7:11016. doi: 10

Insert

KmTOR

Tags

ZZ-3xTEV

Expression

Yeast

Mutation

aa 4-4609

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO524

Plasmid

#235744

Purpose

Protein expression of KmLst8 by integrating KmLST8 into the K. marxianus genome

Depositor

Roger Williams

Article

Baretic et al Nat Commun. 2016 Apr 13;7:11016. doi: 10

Insert

KmLST8

Expression

Yeast

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGADT7-RWT4Ca_Reg1

Plasmid

#237525

Purpose

Yeast two-hybrid

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca_Reg1

Expression

Yeast

Mutation

Removed binding region 1

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGADT7-RWT4Ca+C-Tail

Plasmid

#237526

Purpose

Yeast two-hybrid

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca+C-Tail

Expression

Yeast

Mutation

Add C-terminal extension

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGADT7-RWT4Ca

Plasmid

#237524

Purpose

Yeast two-hybrid

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca

Expression

Yeast

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGBKT7-AvrPWT4

Plasmid

#237527

Purpose

Yeast two-hybrid

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

AvrPwt4

Expression

Yeast

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGBKT7-VirPWT4

Plasmid

#237528

Purpose

Yeast two-hybrid

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

VirPwt4

Expression

Yeast

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pML107-NET1

Plasmid

#232893

Purpose

Plasmid expressing Cas9 and gRNA GTGCCACTAATGGATCCATG which targets the NET1 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

NET1 gRNA (NET1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pML107-KAE1

Plasmid

#232891

Purpose

Plasmid expressing Cas9 and gRNA GATGACAACTGAATGCAGAG which targets the KAE1 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

KAE1 gRNA (KAE1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pML107-VRG4

Plasmid

#232899

Purpose

Plasmid expressing Cas9 and gRNA TCGCATAGCCCAGTATACGT which targets the VRG4 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

VRG4 gRNA (VRG4 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pML107-SAM50

Plasmid

#232895

Purpose

Plasmid expressing Cas9 and gRNA AATAGTTTATGTAAGAACAG which targets the SAM50 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

SAM50 gRNA (SAM50 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO1920

Plasmid

#235767

Purpose

Protein expression of ScVPS34 untagged + ScVPS15 S622D_H623E_T626E-ZZ in yeast

Depositor

Roger Williams

Article

Špokaitė et al eLife

Inserts

VPS34 (VPS34 Budding Yeast)
VPS15 (VPS15 Budding Yeast)

Tags

3xTEV-ZZ

Expression

Yeast

Mutation

S622D, H623E, T626E and ScVPS15 S622D_H623E_T626E

Promoter

GAL-TDH3

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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