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pLenti-hSyn-Gαi*-BERKY1

Plasmid

#158427

Purpose

BRET biosensor for detection of Gαi-GTP in lentiviral vector pLenti-hSyn

Depositor

Mikel Garcia-Marcos

Publication

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-YFP-KB1753-myc

Use

Lentiviral

Tags

myc

Expression

Mammalian

Promoter

hSynapsin

Available since

Sept. 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1-Gαi*-BERKY2

Plasmid

#158197

Purpose

BRET biosensor for detection of Gαi-GTP in mammalian expression vector pcDNA3.1(+)

Depositor

Mikel Garcia-Marcos

Publication

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Lyn11-Nluc-ER/K linker-2xYFP-KB1753-myc

Tags

myc

Expression

Mammalian

Promoter

CMV

Available since

Sept. 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1-Gαi*-BERKY1

Plasmid

#158090

Purpose

BRET biosensor for detection of Gαi-GTP in mammalian expression vector pcDNA3.1(+)

Depositor

Mikel Garcia-Marcos

Publication

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Lyn11-Nluc-ER/K linker-YFP-KB1753-myc

Tags

myc

Expression

Mammalian

Promoter

CMV

Available since

Sept. 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pMC060-HisMS2_PLP_Env_pac

Plasmid

#155040

Purpose

Generation of MS2 Virus Like Particles packaged with the sequence for the SARS-CoV-2 Envelope Gene

Depositor

Paul Freemont

Publication

Crone et al Nat Commun. 2020 Sep 8;11(1):4464. doi:

Inserts

Maturation Protein
Coat Protein Dimer
Envelope Gene

Expression

Bacterial

Mutation

Remove TypeIIs Restriction Sites

Promoter

T7

Available since

Sept. 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV2 TEV-N

Plasmid

#154424

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

N (N SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector, with insert of n…

Mutation

Many synonymous changes due to codon optimization

Available since

Sept. 2, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-ACE2

Plasmid

#158381

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ACE2 (ACE2 Human)

Tags

MAC-tag

Expression

Mammalian

Available since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-SARS-CoV-2 NSP8

Plasmid

#158359

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SARS-CoV-2 NSP8 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-SARS-CoV-2 NSP10

Plasmid

#158361

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SARS-CoV-2 NSP10 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp14-HF

Plasmid

#157719

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp14 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Publication

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Nsp14-HF (ORF1ab SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Available since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp12

Plasmid

#157709

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Publication

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp12 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp9-HF

Plasmid

#157705

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp9 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Publication

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Nsp9-HF (ORF1ab SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp9

Plasmid

#157703

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp9 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Publication

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp9 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA-FRT-TO-FH-Nsp8-Nsp7_GC3opt

Plasmid

#157701

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Publication

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FH-Nsp8-Nsp7_GC3opt (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp8-HF_GC3opt

Plasmid

#157699

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Publication

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Nsp8-HF_GC3opt (ORF1ab SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp8

Plasmid

#157694

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Publication

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp8 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pcDNA5-FRT-TO-Nsp7-HF

Plasmid

#157690

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp7 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Publication

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Nsp7-HF (ORF1ab SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp1-HF

Plasmid

#157681

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp1 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Publication

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HF-Nsp1 (ORF1ab SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Available since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-FH-Nsp1

Plasmid

#157680

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp1 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Publication

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FH-Nsp1 (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Available since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4258326

Plasmid

#154793

Purpose

Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

Depositor

Ginkgo Bioworks, Benjie Chen

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SARS-CoV-2 surface glycoprotein (Spike protein) (S Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;V5

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133280

Plasmid

#152958

Purpose

Mammalian expression plasmid for SARS-CoV-2 N (nucleocapsid)

Depositor

Ginkgo Bioworks, Benjie Chen

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;V5

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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