-
Plasmid#103056Purposemedium copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)DepositorInsertTIMER-bac
UseTagsExpressionBacterialMutationmutations: S4T and S179TPromoterconstitutive ybaJp promoterAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBR322-MCK1.35lux
Plasmid#53368Purposeexpresses luciferase under 1.35 kb muscle creatine kinase promoter/enhancerDepositorInsertmuscle creatine kinase promoter/enhancer 1.35kb
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBR322-bom
Plasmid#155178PurposepBICI_2_strong without bom siteDepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
DVK_pBR322_FG
Plasmid#217595PurposeDestination vector with kanamycin resistance and pBR322 origin of replication. Carries F-G CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVK_pBR322_AF
Plasmid#217593PurposeDestination vector with kanamycin resistance and pBR322 origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVA_pBR322_AH
Plasmid#217606PurposeDestination vector with ampicillin resistance and pBR322 origin of replication. Carries A-H CIDAR MoClo overhangs for level 0 or 2 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVK_pBR322_GH
Plasmid#217596PurposeDestination vector with kanamycin resistance and pBR322 origin of replication. Carries G-H CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBR322_KanR_AgeI_AvrII_PSE1
Plasmid#135229PurposePSE1 experimental evolutionDepositorInsertPSE-1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVA_pBR322_AG
Plasmid#217607PurposeDestination vector with ampicillin resistance and pBR322 origin of replication. Carries A-G CIDAR MoClo overhangs for level 0 or 2 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVC_pBR322_AF
Plasmid#217598PurposeDestination vector with chloramphenicol resistance and pBR322 origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVC_pBR322_EF
Plasmid#217599PurposeDestination vector with chloramphenicol resistance and pBR322 origin of replication. Carries E-F CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVC_pBR322_FG
Plasmid#217600PurposeDestination vector with chloramphenicol resistance and pBR322 origin of replication. Carries F-G CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVC_pBR322_GH
Plasmid#217601PurposeDestination vector with chloramphenicol resistance and pBR322 origin of replication. Carries G-H CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVC_pBR322_AE
Plasmid#217597PurposeDestination vector with chloramphenicol resistance and pBR322 origin of replication. Carries A-E CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVA_pBR322_AF
Plasmid#217608PurposeDestination vector with ampicillin resistance and pBR322 origin of replication. Carries A-F CIDAR MoClo overhangs for level 0 or 2 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVA_pBR322_EH
Plasmid#217609PurposeDestination vector with ampicillin resistance and pBR322 origin of replication. Carries E-H CIDAR MoClo overhangs for level 0 or 2 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBR322-PVCpnf17-22
Plasmid#198272PurposeUnmodified pPayload; contains WT PVC payloads/regulatory genesDepositorInsertPVCpnf17-22
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBR322-PVCpnf18-21
Plasmid#198273PurposeContains only regulatory genes; for generating empty PVCsDepositorInsertPVCpnf18-21
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBR322 14-3-3 Promoter
Plasmid#16517DepositorInsert14-3-3 promoter (SFN Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBR322-Pdp1_NTD-Cre
Plasmid#198274PurposeFor loading Cre into PVCs; also contains PVC regulatory genesDepositorInsertPdp1_NTD-Cre
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
p0857 pBR322 SV40
Plasmid#10877DepositorInsertSV40
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
pBR322-Pdp1_NTD-Cas9
Plasmid#198275PurposeFor loading SpCas9 into PVCs; also contains PVC regulatory genesDepositorInsertPdp1_NTD-Cas9
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBR322-Pdp1-HiBiT
Plasmid#198310PurposeLoads HiBiT-tagged Pdp1 into PVCs; for payload loading assays; also contains PnfDepositorInsertPdp1-HiBiT
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBR322-Pdp1_NTD-ZFD_L
Plasmid#198276PurposeFor loading L half of zinc finger deaminase targeting human TRAC locus (ZFD_TRAC-NC-Left-G1397-N_DddA); also contains PVC regulatory genesDepositorInsertPdp1_NTD-ZFD_L
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBR322-Pdp1_NTD-ZFD_R
Plasmid#198277PurposeFor loading R half of zinc finger deaminase targeting human TRAC locus (ZFD_TRAC-NC-Right-G1397-C_DddA); also contains PVC regulatory genesDepositorInsertPdp1_NTD-ZFD_R
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBR322-Pdp1_aa1-38-HiBiT
Plasmid#198315PurposeLoads a truncated payload; for identification of Pdp1 packaging domainDepositorInsertPdp1_aa1-38-HiBiT
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBR322-Pdp1_aa1-76-HiBiT
Plasmid#198314PurposeLoads a truncated payload; for identification of Pdp1 packaging domainDepositorInsertPdp1_aa1-76-HiBiT
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBR322 hCDK4 promoter
Plasmid#16571DepositorInsertCDK4 promoter (CDK4 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBR322 mCDK4 promoter
Plasmid#16572DepositorInsertCDK4 promoter (Cdk4 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pXTK021 [Origin_pBR322-ROP_PartType1]
Plasmid#229258PurposeXanthoMoClo Golden Gate Part Plasmid for cloning, contains Origin_pBR322-ROP_PartType1DepositorInsertpBR322-ROP Origin
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBR322_ZA-ampR-PSEAB-2A
Plasmid#135230PurposeAAC6 experimental evolutionDepositorInsertaac6 Pseudomonas sp. ABAC61
UseTagsExpressionBacterialMutationPromoterpZAAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL0_26 [bla pBR322]
Plasmid#198939PurposeLevel 0 partDepositorInsertbla pBR322
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBR322-Pdp1_NTD-GFP-HiBiT
Plasmid#198317PurposeLoads HiBiT-tagged GFP into PVCs using Pdp1 packaging domain; also contains PnfDepositorInsertPdp1_NTD-GFP-HiBiT
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBR322-Pdp1_NTD-Cre-HiBiT
Plasmid#198318PurposeLoads HiBiT-tagged Cre into PVCs using Pdp1 packaging domain; also contains PnfDepositorInsertPdp1_NTD-Cre-HiBiT
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBR322-Pdp1_NTD-ZFN_L-HiBiT
Plasmid#198319PurposeLoads HiBiT-tagged L fragment of a zinc finger nuclease specific for human AAVS1 locus using Pdp1 packaging domainDepositorInsertPdp1_NTD-ZFN_L-HiBiT
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBR322-Pdp1_aa39-331-HiBiT
Plasmid#198316PurposeLoads a truncated payload; for identification of Pdp1 packaging domainDepositorInsertPdp1_aa39-331-HiBiT
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBR322-Pdp1_aa1-133-HiBiT
Plasmid#198313PurposeLoads a truncated payload; for identification of Pdp1 packaging domainDepositorInsertPdp1_aa1-133-HiBiT
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBR322-Pdp1_aa1-289-HiBiT
Plasmid#198311PurposeLoads a truncated payload; for identification of Pdp1 packaging domainDepositorInsertPdp1_aa1-289-HiBiT
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBR322-Pdp1_aa1-190-HiBiT
Plasmid#198312PurposeLoads a truncated payload; for identification of Pdp1 packaging domainDepositorInsertPdp1_aa1-190-HiBiT
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pC008 - pBR322 with tetR-inducible RFP
Plasmid#79157Purposeinducible RFP expression vectorDepositorInsertTetR + RFP
UseTagsExpressionMutationPromoterAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHS0535 CRISPR-associated IscB (Chesapeake Bay) in pBR322 with Fn spacer
Plasmid#176589PurposeEndogenous locus of CRISPR-associated IscB (Chesapeake Bay) with Fn spacerDepositorInsertCRISPR-associate IscB (Chesapeake Bay) locus
UseTagsExpressionBacterialMutationTruncated CRISPR array to two direct repeats, rep…PromoterAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHS0537 CRISPR-associated IscB locus (Delaware Bay) in pBR322 with Fn spacer
Plasmid#176588PurposeEndogenous locus of CRISPR-associated IscB (Delaware Bay) with Fn spacerDepositorInsertCRISPR-associate IscB (Delaware Bay) locus
UseTagsExpressionBacterialMutationReplaced spacer in CRISPR array with Fn spacerPromoterAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPtPBR2
Plasmid#80387PurposeEpisome vector for Phaeodactylum tricornutum (pBR322-based)DepositorTypeEmpty backboneUseSynthetic Biology; ; episomal vector for phaeodac…TagsExpressionMutationPromoterAvailable sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPtPBR11
Plasmid#80386PurposeEpisome vector for Phaeodactylum tricornutum (pBR322-based)DepositorTypeEmpty backboneUseSynthetic Biology; : episomal vector for phaeodac…TagsExpressionMutationPromoterAvailable sinceSept. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPtPBR1
Plasmid#80388PurposeEpisome vector for Phaeodactylum tricornutum (pBR322-based)DepositorTypeEmpty backboneUseSynthetic Biology; ; episomal vector for phaeodac…TagsExpressionMutationPromoterAvailable sinceSept. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pES133
Plasmid#127552PurposeVariant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F5-tetA-F5 cassette orthogonal to FRT-KanR-FRT in pKD13DepositorInsertF5-flanked tetracycline resistance cassette
UseTagsExpressionBacterialMutationMultiple SNPs in tetA (originally from pBR322) to…PromoterAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBR HPV31 9E
Plasmid#221009PurposeHuman papillomavirus 31 isolate 9E, complete genome cloned in pBR322DepositorInsertHuman papillomavirus type 31 genome 9E isolate
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPtGG-1
Plasmid#89990PurposepBR322-based entry vector for Gateway cloningDepositorTypeEmpty backboneUseGolden gate entry vectorTagsExpressionMutationPromoterAvailable sinceJune 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCVD502
Plasmid#78570PurposeA 5.6 kb Sa1I fragment carryig the 1st tdh gene copy was cloned from pCVD501 into the Sa1I site of pBR322.DepositorInserttdh1
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJuly 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMK188
Plasmid#226659PurposepCB2.4 - pBR322 shuttle vector that replicates in E. coli and Synechococcus sp. PCC 7002, contains HIP1 siteDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only