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Plasmid#48982PurposeSupplies the selection marker (blasticidin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).DepositorInsertblasticidin-S deaminase
UseGateway cloning vectorTagsIRES (weak)ExpressionMutationPromoternaAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
G384A_pLenti-TetBattP(GT)-BFP-2A-iCasp9-2A-Blast_rtTA3
Plasmid#200630PurposeNegative selection blasticidin resistant Tet-inducible Matreyek Bxb1(GT) landing padDepositorInsertiCasp9
UseLentiviralTagsExpressionMutationPromoterpTRE3GAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
G417A_pLenti-Tet-BattP(GA)-BFP-2A-iCasp9-2A-Blast_rtTA3
Plasmid#200631PurposeNegative selection blasticidin resistant Tet-inducible Matreyek Bxb1(GA) landing padDepositorInsertiCasp9
UseLentiviralTagsExpressionMutationPromoterpTRE3GAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
MMLV-CAG-G_oT7pol-IRES-BSD
Plasmid#172369PurposeGeneration of oT7pol-expressing stable cell lines, resistant to the antibiotic BlasticidinDepositorInsertoT7pol + BSD
UseRetroviralTagsExpressionMammalianMutationPromoterCAGAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2-Blast-mU6
Plasmid#206806PurposepLentiCRISPRv2 variant with mU6 promoter driving the sgRNA, and a Blasticidin resistance cassetteDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBigTB
Plasmid#149432Purposeshuttle vector with blasticidin-S resistance into ROSA26 targetting vectorsDepositorTypeEmpty backboneUseCre/Lox and Mouse TargetingTagsExpressionMammalianMutationPromoterSV40Available sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
PMXS-GOT1
Plasmid#72872PurposeThe retroviral GOT1 vector was generated by cloning an sgRNA resistant human GOT1 gene block into the pMXS-ires-blast vector by Gibson Assembly.DepositorInsertGOT1 (GOT1 Human)
UseRetroviralTagsExpressionMammalianMutationsgRNA resistant human GOT1, 7 silent mutations c1…PromoterAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDRM98 mKate2 Blast
Plasmid#174720PurposeExpresses the NLS-tagged mKate2 gene, P2A and the blasticidin resistance gene.DepositorInsertsmKate2
BSD
UseLentiviralTagsP2A linker and SV40 NLS with GGS linkerExpressionMammalianMutationPromoterMND and MND (off mKate-P2A)Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLIB-TetR-KRAB-IRES-BLAS
Plasmid#55820Purposeretroviral bicistronic expression vector for TetR-KRAB and Blasticidin S resistanceDepositorInsertTetR-KRAB
UseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
Lenti-dCas9-KRAB-blast
Plasmid#89567PurposePlasmid expression dCas9 protein in fusion with KRAB domainDepositorInsertdCas9
UseLentiviralTagsBlasticidin resistance gene and KRABExpressionMutationD10A and H840APromoterAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only