-
Plasmid#224509PurposeGateway compatible middle entry clone containing a Myc tagged BirADepositorInsertMyc-BirA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Myc-BirA*-RNF8-C403S
Plasmid#221685PurposeMammalian expression of Myc-tagged BirA*-RNF8 (C403S) fusion protein for BioIDDepositorInsertRNF8 (RNF8 Human)
UseTagsExpressionMammalianMutationC403SPromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Myc-BirA*-RNF8-R42A
Plasmid#221684PurposeMammalian expression of Myc-tagged BirA*-RNF8 (R42A) fusion protein for BioIDDepositorInsertRNF8 (RNF8 Human)
UseTagsExpressionMammalianMutationR42APromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-N-FLAG-hBirA*-TLK1-WT
Plasmid#218834PurposeExpresses N-terminal BirA*-tagged human wild type TLK1 variant 5 for BioID experiments.DepositorInsertTLK1 (TLK1 Human)
UseTagsFLAG-BirA*ExpressionMammalianMutationPromoterCMVAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
3'-BirA*-FLAG minimal CMV pCDNA5
Plasmid#212047PurposeLR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locusDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
5'-BirA*-FLAG minimal CMV pCDNA5
Plasmid#212046PurposeLR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locusDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLIK BirA* 3xFLAG-NUP37 zeo
Plasmid#192333PurposeLentiviral expression vector for an inducible 3xFLAG-BirA 3xFLAG-NUP37DepositorInsertBirA*-3xFLAG-NUP37 (NUP37 Human)
UseLentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLIK BirA* 3xFLAG-CSE1L zeo
Plasmid#192334PurposeLentiviral expression vector for an inducible 3xFLAG-BirA 3xFLAG-CSE1LDepositorInsertBirA*-3xFLAG-CSE1L (CSE1L Human)
UseLentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MCS-BirA(R118G)-HA-TARG1
Plasmid#172583PurposeFor transient expression of C-terminal BirA-tagged TARG1DepositorInsertTARG1 (OARD1 Human)
UseTagsBirA(R118G)-HAExpressionMammalianMutationPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MCS-BirA(R118G)-HA-MACROD2
Plasmid#172582PurposeFor transient expression of C-terminal BirA-tagged MACROD2DepositorInsertMACROD2 (MACROD2 Human)
UseTagsBirA(R118G)-HAExpressionMammalianMutationPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 rtTA BirA eIF4G +MIC
Plasmid#158790PurposepcDNA5 based transfection plasmid for the exogenous expression of N-terminal BirA-Flag-tagged eIF4G1 including the microexon for generation of N2A FlpIn cell lines for BioIDDepositorInserteIF4G1 (with microexon)
UseTags3xFlag and BirA*ExpressionMammalianMutationPromoterminiCMVAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3/flag-pBirA(1-K283)-FRB
Plasmid#154451Purposeexpresses flag-pBirA(1-K283)-FRB/biochemical assayDepositorInsertflag-pBirA(1-K283)-FRB
UseTagsExpressionMammalianMutationPromoterT7 promoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_3C-Avi-His6_IRES-HA-BirA-ER
Plasmid#113898PurposeMammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of HA-tagged ER-resident E. coli biotin ligaseDepositorTypeEmpty backboneUseLentiviralTags3C-Avi-His6ExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
Affibody HER2:243.BirA*-pET301/NT-DEST
Plasmid#174692PurposePlasmid encoding for the bacterial expression of a bispecific coding for the anti-HER2 affibody ZHER2:342 fused to the mutated form of BirA enzyme, BirA*DepositorInsertAnti-HER2 affibody ZHER2:342 fused to mutated form of BirA, BirA*
UseTagsHIS tagExpressionBacterialMutationR118G mutation in BirAPromoterT7 promoterAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLEX-HA-birA*-K-Ras(G12D)-IRES-Puro
Plasmid#120562PurposeExpresses HA-birA*-K-Ras G12D fusion protein in mammalian cells & for virus productionDepositorInsertKRAS4B (KRAS Human)
UseLentiviralTagsHA-birA*ExpressionMammalianMutationG12DPromoterCMVAvailable sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 - PP1g - Flag - BirA* (AA2-140)
Plasmid#86886PurposeN-terminal part of the split-BioIDDepositorInsertPP1g - FLAG - BirA (AA2-140)
UseTagsFLAG tagExpressionMammalianMutationBirA Amino Acids 2-140PromoterAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLEX-HA-birA*-K-Ras(wildtype)-IRES-Puro
Plasmid#120561PurposeExpresses HA-birA*-K-Ras wildtype fusion protein in mammalian cells & for virus productionDepositorInsertKRAS4B (KRAS Human)
UseLentiviralTagsHA-birA*ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
phage - ubc - nls - 2xmcp - egfp - BirA*
Plasmid#131132PurposeBirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.DepositorInsertNLS-HA-2xMCP-EGFP-BirA*
UseLentiviralTagsNLS, HAExpressionMammalianMutationBirA* = BIrA >R118GPromoterhuman ubiquitin C (UBC)Available sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
phage - ubc - nls - mcp - egfp - BirA*
Plasmid#131134PurposeBirA* expressed as a protein fusion with MS2 coat protein (MCP) monomer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.DepositorInsertNLS-HA-MCP-EGFP-BirA*
UseLentiviralTagsNLS, HAExpressionMammalianMutationBirA* = BIrA >R118GPromoterhuman ubiquitin C (UBC)Available sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLEX-uORF-HA-birA*-STOP-IRES-Puro
Plasmid#120558PurposeExpresses control HA-birA* in mammalian cells & for virus productionDepositorInsertbirA
UseLentiviralTagsHAExpressionMammalianMutationR118GPromoterCMVAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only