We narrowed to 235 results for: PGK1
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Plasmid#185891PurposeIn vivo gene amplification (HamAmp) of AeBlue expression module at RPL25 locusDepositorInsertPRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>AeBlue>TPGK1- PBTS1> RPL25(partial; Arm2)
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIEforRed
Plasmid#185892PurposeIn vivo gene amplification (HamAmp) of EforRed expression module at RPL25 locusDepositorInsertPRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>EforRed>TPGK1-PBTS1> RPL25(partial; Arm2)
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_H2BK_mNG_hSLBP(18-108)
Plasmid#191529PurposeDonor for targeted integration of mNG_hSLBP(18-108) probe to the AAVS1 safe harbor locusDepositorInsertH2BK_mNG_hSLBP(18-108)
UseCRISPR and Synthetic BiologyTagsmNeonGreen (mNG)ExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIAeBlueHPV16LR
Plasmid#185894PurposeIn vivo gene amplification (HamAmp) of AeBlue + HPV16 L1 expression module at RPL25 locusDepositorInsertPRPL25(Arm1)>Kl.LEU2>TKl.LEU2-TRPL25(Arm3)-ARS305-PALD6>EforRed>TPGK1-PSe.GAL2>HPV16-L1_C-6*H>TRPL41B-PBTS1>RPL25(partial;Arm2)
UseTagsExpressionYeastMutationNonePromoterAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSSa26
Plasmid#186784PurposeExpression of reverse transactivator (CgrtTA)DepositorInsertCgrtTA
UseTagsExpressionYeastMutationPromoterScPGK1pAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pTRK669
Plasmid#71313PurposeHelper plasmid for pORI plasmids. Chloramphenicol resistant, temperature sensitive replication in E. coli and gram positives. Encodes RepA.DepositorTypeEmpty backboneUseE. coli-gram positive bacteria shuttle vectorTagsExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGK415-rtetTAK8N, L131L
Plasmid#165969PurposeExpresses rTetTA (K8N, L131L) mutantDepositorInserttetR
UseSynthetic BiologyTagsthree copies of VP16 (VP48)ExpressionYeastMutationPromoterpPGK1Available sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
S3_001_026_MV2
Plasmid#134422PurposepYTKMV2-pTDH3_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
NLS_FdeR-N
UseTagsnoneExpressionYeastMutationnonePromoterpGMP1 and pTDH3Available sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS403red-luxTA2-4F
Plasmid#166030PurposeExpresses luxTA(2-4F) mutantDepositorInsertluxR
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationS116Y, W201R, codon-optimized for expression in y…PromoterpPGK1 mutantAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405red-phlTA2-1E
Plasmid#166029PurposeExpresses phlTA(2-1E) mutantDepositorInsertphlF
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationP5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…PromoterpPGK1 mutantAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGK415-luxTA2-4F
Plasmid#165971PurposeExpresses luxTA(2-4F) mutantDepositorInsertluxR
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationS116Y, W201R, codon-optimized for expression in y…PromoterpPGK1 mutantAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3A2 (2)
Plasmid#185852PurposeTesting the TEF1 promoter appended with 3 tetracycline riboswitch using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1>3*TcRb>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGK415-phlTA2-1E
Plasmid#165970PurposeExpresses phlTA(2-1E) mutantDepositorInsertphlF
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationP5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…PromoterpPGK1 mutantAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pILGFP9E3
Plasmid#185842PurposeTesting the TEF1 promoter inserted with 4 TetO elements using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1+[TetO]>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
S3_002_026_096
Plasmid#134403PurposepYTK096-pRPL18B_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
NLS_FdeR-N
UseTagsNone and noneExpressionYeastMutationNone and nonePromoterpGPM1 and pRPL18BAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAN1935
Plasmid#127203PurposeConstitutive expression of Venus-degronSwitchA and mScarlet-degronSwitchCDepositorInsertpTEF1-Venus-degronSwitch_A_t12-tENO2-pTEF2-mScarlet-Linker-degronSwitch_C-tPGK1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
S3_004_026_MV1
Plasmid#134416PurposepYTKMV1-pTDH3_FdeR-N_2NLS_tPGK1-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
FdeR-N_2xNLS
UseTagsnoneExpressionYeastMutationnonePromoterpGMP1 and pTDH3Available sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4D42
Plasmid#185840PurposeTesting the TEF1 promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1+[Z268]>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3A2 (1)
Plasmid#185851PurposeTesting the TEF1 promoter appended with 2 tetracycline riboswitch using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1>2*TcRb>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only