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Plasmid#112020PurposeDNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with BFPDepositorInsertCD4-BFP HDRT (CD4 Human)
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
CD4-C-mCherry HDRT Source (pTR 175)
Plasmid#112019PurposeDNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with mCherryDepositorInsertCD4-mCherry HDRT (CD4 Human)
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pONSY-Lifeact:mCherry
Plasmid#111876PurposeThis plasmid expresses mCherry fused to Lifeact peptide. It labels actin bundles and filopodia in Capsaspora. Codons have been optimised according to their usages in Capsaspora and C. fragrantissima.DepositorInsertLifeact fused to mCherry
UseCapsaspora owczarzakiTagsmCherryExpressionMutationPromoterElongation Factor 1 alpha (EF1a) from CapsasporaAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FKBP-C-mSOG-J-alpha-IRES-mCherry
Plasmid#136603PurposeEncodes FKBP tagged with the C-terminal fragment of split-miniSOG (mSOG-Ja95-140).DepositorInsertFKBP-C-mSOG-J-alpha
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK371
Plasmid#73596PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-3) with C-terminal c-myc epitopeDepositorInsertsdelta-12 fatty acid acetylenase
delta-12 fatty acid desaturase (form 3)
UseTagsFLAG tag and c-myc tagExpressionYeastMutationPoint mutants in FAD2-3 ORF relative to deposited…PromoterGAL1 and GAL10Available sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCG_SARS-CoV2/bat-CoV RaTG13 E C-V5-tag
Plasmid#179977PurposeMammalian expression vector for SARS-CoV-2 E or bat RaTG13 E, V5-tagged (SARS-CoV-2 E and RaTG13 E have the same amino acid sequence and codon optimized sequence).DepositorInsertSARS-CoV-2 E (E Synthetic)
UseTagsExpressionMammalianMutationhuman codon-optimizedPromoterAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCG_SARS-CoV2 NSP7/bat-CoV RaTG13 C-V5-tag
Plasmid#179974PurposeMammalian expression vector for SARS-CoV-2 Nsp7 or bat RaTG13 Nsp7, V5-tagged (SARS-CoV-2 Nsp7 and RaTG13 Nsp7 have the same amino acid sequence and codon optimized sequence).DepositorInsertSARS-CoV-2 Nsp7 (ORF1ab Synthetic)
UseTagsExpressionMammalianMutationhuman codon-optimizedPromoterAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEM cWnt14 (CT#692)
Plasmid#13947DepositorInsertWnt-14 (WNT9A Chicken)
UseTagsExpressionBacterialMutationNote that this insert is in the reverse direction…PromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCR2.1_HBB_N
Plasmid#87847PurposepCR2.1-based plasmid holding a 309-bp fragment containing the exon-1-exon-2 splice border of normal human β-globin mRNADepositorInsertHomo sapiens hemoglobin subunit beta (HBB), Normal cDNA fragment (Exon1/2) (HBB Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBV-Luc/Del-6
Plasmid#14969DepositorInsertc-myc promoter (-109/+334) (MYC Human)
UseLuciferaseTagsExpressionMammalianMutation-109/+334 (relative to the P2 transcription initi…PromoterAvailable sinceMay 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBV-Luc/Del-7
Plasmid#14968DepositorInsertc-myc promoter (-26/+334) (MYC Human)
UseLuciferaseTagsExpressionMammalianMutation-26/+334 (relative to the P2 transcription initia…PromoterAvailable sinceMay 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBV-Luc/Frag-F
Plasmid#14053DepositorInsertc-myc promoter (-109/-3) (MYC Human)
UseLuciferaseTagsExpressionMammalianMutation-109/-3 (relative to the P2 transcription initiat…PromoterAvailable sinceMay 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCR2.1_HBB_A
Plasmid#87848PurposepCR2.1-based plasmid holding a 328-bp fragment containing the exon-1-exon-2 splice border of aberrant HBB[IVSI-110(G>A)] human β-globin mRNADepositorInsertHomo sapiens hemoglobin subunit beta (HBB), Aberrant cDNA fragment (Exon1/19nt mispliced Intron1 + Exon2) (HBB Human)
UseTagsExpressionBacterialMutationHBB(IVSI-110) β-thalassaemia misplicing mutation …PromoterAvailable sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKG139
Plasmid#69755PurposeRetroviral expression of CENP-CDepositorInsertCENP-C (CENPC Human)
UseRetroviralTagsmCherry-TEV-SExpressionMammalianMutationL341F, N570D relative to NP_001803.2 (numbering b…PromoterAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3-FLIP pr5
Plasmid#19130DepositorInsertFLIP promoter (CFLAR Human)
UseLuciferaseTagsExpressionMammalianMutationThe sequence corresponds to positions -503 to +28…PromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGL3-FLIP pr4
Plasmid#19129DepositorInsertFLIP promoter (CFLAR Human)
UseLuciferaseTagsExpressionMammalianMutationThe sequence corresponds to positions -503 to +10…PromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
cfSGFP2-N
Plasmid#37533DepositorInsertcysteine-free SGFP2-N
UseTagsExpressionMammalianMutationC48S and C70M mutations relative to SGFP2PromoterCMVAvailable sinceAug. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Myc CDC4 WT*
Plasmid#16652DepositorInsertCDC4 (FBXW7 Human)
UseTagsmycExpressionMammalianMutation*Note that this insert is only WT relative to the…PromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
lenti.DTR.GFP
Plasmid#201962PurposeLentiviral vector that expresses diptheria toxin receptor (DTR) C-terminally fused to EGFPDepositorInsertDiptheria Toxin Receptor
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1alphaAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57-Cx46-IRES-GCaMP6s
Plasmid#188238Purpose3rd generation, inducible bicistronic lentiviral plasmid for expression of human connexin 46 and GCaMP6sDepositorInsertGJA3 (GJA3 Human)
UseLentiviralTagsGCaMp6sExpressionMammalianMutationPromoterTight TRE promoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only