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Showing: 181 - 200 of 501 results

  7. pPP381

    Plasmid
    #158798
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-2(D394A) in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Article
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    dCasPhi-2
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    RuvC-inactivating mutation D394A
    Promoter
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158798-plasmid-map-sequence-id-309523

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  9. pPP537

    Plasmid
    #158803
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally 2xSV40 NLS and hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Article
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    CasPhi-2
    Use
    Tags
    2xSV40NLS-6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158803-plasmid-map-sequence-id-309579

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  10. B52_puro_empty_gRNA

    Plasmid
    #197557
    Purpose
    sites for cloning two gRNA with puromycin cassette
    Depositor
    Darius Bägli
    Type
    Empty backbone
    Use
    Empty grna backbone with puromycin resistance gene
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 for gRNA, CMV for puromycin resistance.
    Available since
    March 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    197557-plasmid-map-sequence-id-388707

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  13. ER2-hfYFP

    Plasmid
    #186518
    Purpose
    Localization and retention of hyperfolder YFP fluorescent protein in the ER; improved targeting efficiency
    Depositor
    Gregory Petsko
    Article
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Insert
    Hyperfolder YFP
    Use
    Tags
    Calreticulin and KDEL
    Expression
    Mammalian
    Mutation
    mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…
    Promoter
    CMV
    Available since
    Nov. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    186518-plasmid-map-sequence-id-368092

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  15. pCytERM-mhYFP

    Plasmid
    #186530
    Purpose
    Visualization of the ER in cells using monomeric hyperfolder YFP (compatible with OSER assay)
    Depositor
    Gregory Petsko
    Article
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Insert
    Monomeric hyperfolder YFP
    Use
    Tags
    Cytochrome p450 first 29 animo acids (Oryctolagus…
    Expression
    Mammalian
    Mutation
    hfYFP-S147P/L195M/V206K
    Promoter
    CMV
    Available since
    Nov. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    186530-plasmid-map-sequence-id-368240

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Showing: 181 - 200 of 501 results