We narrowed to 577 results for: hyper

1. pL-rpb1-E1103G

   Plasmid

   #10989

   Depositor

   Jeff Strathern

   Article

   Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

   Insert

   rpb1-E1103G (RPO21 Budding Yeast)
   

   Expression

   Bacterial and Yeast

   Mutation

   Mutation 3308 A to G in the RPB1 ORF resulting in…

   Available Since

   Dec. 16, 2005

   Availability

   Academic Institutions and Nonprofits only

2. PBAD-his6-prkA-pACYC184

   Plasmid

   #41041

   Depositor

   Ichiro Matsumura

   Article

   Parikh et al Protein Eng Des Sel. 2006 Mar;19(3):113-

   Insert

   prkA
   

   Tags

   6xHis and T7 epitope tag

   Expression

   Bacterial

   Promoter

   PBAD

   Available Since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. CMV-enAsCas12f

   Plasmid

   #204637

   Purpose

   Expression of enAsCas12f in mammalian cells

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

   Insert

   enAsCas12f
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   D196K, N199K, G276R, N328G, D364R

   Promoter

   CMV

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGH224_sgRNA_2xMS2_Puro

   Plasmid

   #85413

   Purpose

   hU6 driven sgRNA vector with 2xMS2 vectors with puro selectable marker

   Depositor

   Michael Bassik

   Article

   Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. U6-AsCas12f-full-length-sgRNA

   Plasmid

   #204638

   Purpose

   Expression of full-length AsCas12f sgRNA in mammalian cells

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

   Insert

   full-length AsCas12f sgRNA
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. U6-AsCas12f-sgRNA-v2

   Plasmid

   #204639

   Purpose

   Expression of trancated AsCas12f sgRNA in mammalian cells

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

   Insert

   trancated AsCas12f sgRNA (sgRNA-v2)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPP089

   Plasmid

   #158796

   Purpose

   pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-3 in MCS1 for expression in E. coli and protein purification

   Depositor

   Jennifer Doudna

   Article

   Pausch et al Science. 2020 Jul 17;369(6501):333-337.

   Insert

   CasPhi-3
   

   Expression

   Bacterial

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. rbcLS-pMAL-2px

   Plasmid

   #41621

   Depositor

   Ichiro Matsumura

   Article

   Parikh et al Protein Eng Des Sel. 2006 Mar;19(3):113-

   Insert

   rbcL/rbcS
   

   Expression

   Bacterial

   Promoter

   Ptac

   Available Since

   Nov. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. LifeAct-mhYFP

   Plasmid

   #186524

   Purpose

   Localization of monomeric hyperfolder YFP fluorescent protein to actin

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   Monomeric hyperfolder YFP
   

   Tags

   LifeAct

   Expression

   Mammalian

   Mutation

   hfYFP-S147P/L195M/V206K

   Promoter

   CMV

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Mito-mhYFP

    Plasmid

    #186526

    Purpose

    Localization of monomeric hyperfolder YFP fluorescent protein to mitochondria

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    Monomeric hyperfolder YFP
    

    Tags

    Human COX8a first 29 amino acids

    Expression

    Mammalian

    Mutation

    hfYFP-S147P/L195M/V206K

    Promoter

    CMV

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Pinducer20-dCas9-SunTag-2A-BFP

    Plasmid

    #115779

    Purpose

    targeted DNA methylation tool

    Depositor

    Margaret Goodell

    Article

    Su et al Genome Biol. 2018 Aug 10;19(1):108. doi:

    Insert

    dCas9-SunTag-2A-BFP
    

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEasy Flox

    Plasmid

    #11725

    Depositor

    Klaus Rajewsky

    Article

    Schenten et al Eur J Immunol. 2002 Nov . 32(11):3152-60

    Insert

    pEasyFlox
    

    Use

    Cre/Lox

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLIC-EcTOP

    Plasmid

    #224463

    Purpose

    Expression of recombinant Escherichia coli topoisomerase I

    Depositor

    Yuk-Ching Tse-Dinh

    Article

    Tan et al Nucleic Acids Res. 2015 Dec 15;43(22):11

    Insert

    Escherichia coli TopA
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPP444

    Plasmid

    #158802

    Purpose

    Plasmid containing Homo sapiens codon optimized CasΦ-3 and a SapI-GG stuffer spacer for editing in human cells.

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    CasPhi-3
    

    Expression

    Mammalian

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCSIIhyg-RGECO1.0

    Plasmid

    #175813

    Purpose

    The lentiviral vector for R-GECO1.0, a genetically encoded red fluorescent Ca2+ sensor.

    Depositor

    Michiyuki Matsuda

    Article

    Hirota et al Endocrinology. 2022 Mar 1;163(3). pii: 6

    Insert

    RGECO1.0
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. ER2-mhYFP

    Plasmid

    #186528

    Purpose

    Localization and retention of monomeric hyperfolder YFP fluorescent protein in the ER; improved targeting efficiency

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    Monomeric hyperfolder YFP
    

    Tags

    Calreticulin and KDEL

    Expression

    Mammalian

    Mutation

    hfYFP-S147P/L195M/V206K

    Promoter

    CMV

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPP394

    Plasmid

    #158800

    Purpose

    Plasmid containing Homo sapiens codon optimized CasΦ-1 and a SapI-GG stuffer spacer for editing in human cells.

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    CasPhi-1
    

    Expression

    Mammalian

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPP076

    Plasmid

    #158794

    Purpose

    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-1 in MCS1 for expression in E. coli and protein purification

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    CasPhi-1
    

    Expression

    Bacterial

    Available Since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCSIIbsr-PKG-hNo.7

    Plasmid

    #175812

    Purpose

    The lentiviral vector for PKG #7, a genetically encoded FRET biosensor for monitoring cGMP concentration.

    Depositor

    Michiyuki Matsuda

    Article

    Hirota et al Endocrinology. 2022 Mar 1;163(3). pii: 6

    Insert

    PKG #7
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Tubulin-mhYFP

    Plasmid

    #186525

    Purpose

    Localization of monomeric hyperfolder YFP fluorescent protein to tubulin

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    Monomeric hyperfolder YFP
    

    Tags

    Tubulin

    Expression

    Mammalian

    Mutation

    hfYFP-S147P/L195M/V206K

    Promoter

    CMV

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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