Skip to main content
Addgene
Showing: 181 - 200 of 310 results
  1. pB PUb-integrase

    Plasmid
    #183966
    Purpose
    piggyBac plasmid for insect transformation, expressing PhiC31 integrase under control of the Aedes aegypti PUb promoter, GFP fluorescent marker
    Depositor
    Insert
    PhiC31 integrase
    Use
    Piggybac insect transgenesis destination vector
    Tags
    Expression
    Mutation
    Promoter
    Aedes aegypti Polyubiquitin
    Available since
    Aug. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. pRDB_051

    Plasmid
    #216081
    Purpose
    for stable fly cell lines; attL and attR sequences for genome integration by phiC31, destination vector
    Depositor
    Has Service
    Cloning Grade DNA
    Insert
    attL and attR sequences for genome integration by phiC31
    Use
    Destination
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. MTK0_011

    Plasmid
    #123966
    Purpose
    Encodes the hROSA26 PhiC31 recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system
    Depositor
    Insert
    hRosa26-PhiC31 landing pad
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
  4. MTK0_052

    Plasmid
    #123978
    Purpose
    Encodes the PhiC31 landing pad on hThal5.10-2-hg18 with hygromycin resistance cassette coexpressed with mRuby2 as a type 0 part to be used in the MTK system
    Depositor
    Insert
    PhiC31 LP on hThal5.10-2-hg18 (Hygro::mRuby)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
  5. MTK0_054

    Plasmid
    #123980
    Purpose
    Encodes the PhiC31 landing pad on hCLYBL with hygromycin resistance cassette coexpressed with mRuby2 as a type 0 part to be used in the MTK system
    Depositor
    Insert
    PhiC31 LP on hCLYBL (Hygro::mRuby)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
  6. pRDB_053

    Plasmid
    #216083
    Purpose
    for transgenic flies; gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31, destination vector
    Depositor
    Has Service
    Cloning Grade DNA
    Insert
    gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31
    Use
    Destination
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. L1T10 - T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)

    Plasmid
    #219687
    Purpose
    Level 1 History-dependent target, switch PhiC31 then Bxb1, pUBQ10 promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).
    Depositor
    Insert
    T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
  8. attB Neo* attP

    Plasmid
    #138412
    Purpose
    attB and attP-flanked pPgk-pEM7-Neo*-bpA positive selection cassette
    Depositor
    Insert
    Neo*
    Use
    Mouse Targeting; Att/phic31
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Nov. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
  9. L1T11 - T2BP/p35S (BFP, mCherry, NeonGreen)

    Plasmid
    #219688
    Purpose
    Level 1 History-dependent target, switch Bxb1 then PhiC31, p35S promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).
    Depositor
    Insert
    T2BP/p35S (BFP, mCherry, NeonGreen)
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
  10. L1T9 - T2PB/p35S (mTagBFP2, mCherry, NeonGreen)

    Plasmid
    #219686
    Purpose
    Level 1 History-dependent target, switch PhiC31 then Bxb1, p35S promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).
    Depositor
    Insert
    T2PB/p35S (mTagBFP2, mCherry, NeonGreen)
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
  11. pDGB3_alpha1_CBS-att-OCS-BeYDVRep-RepA-tNos (GB4643)

    Plasmid
    #225437
    Purpose
    Transcriptional unit for the copper and PhiC31 regulated expression of BeYDV Rep/RepA.
    Depositor
    Insert
    CBS-att-OCS-BeYDVRep/RepA-tNos
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 181 - 200 of 310 results