We narrowed to 10,611 results for: t7

1. pBJG1 T7-8xHis-OGT D554N

   Plasmid

   #154279

   Purpose

   Bacterial expression of full length human OGT with D554N mutation (Cleavage, no Ser/Thr glycosylation). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

   Depositor

   Suzanne Walker

   Article

   Janetzko et al Nat Chem Biol. 2016 Nov;12(11):899-901.

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Tags

   8x His, HRV3C protease site, and T7 leader

   Expression

   Bacterial

   Mutation

   Aspartate 554 changed to Asparagine- bacterial co…

   Available Since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBJG1 T7-8xHis-OGT 5N5A

   Plasmid

   #154280

   Purpose

   Bacterial expression of full length human OGT with 5N5A TPR mutations (inefficient substrate binding). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

   Depositor

   Suzanne Walker

   Article

   Levine et al J Am Chem Soc. 2018 Mar 14;140(10):3510-

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Tags

   8x His, HRV3C protease site, and T7 leader

   Expression

   Bacterial

   Mutation

   N322A, N356A, N390A, N424A, N458A mutations; bact…

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. T7 promoter + rpsL reporter plasmid

   Plasmid

   #156455

   Purpose

   A mutational reporter plasmid that can gain Streptomycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.

   Depositor

   Matthew D. Shoulders

   Article

   Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

   Inserts

   rpsL
   TcR
   

   Expression

   Bacterial

   Mutation

   ATG start codon mutated to ACG

   Promoter

   tac and tet

   Available Since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPGK-T7/2-CD44v3 (-cyt)

   Plasmid

   #137813

   Purpose

   expression of CD44 proteins in mammalian cells

   Depositor

   Ursula Gunthert

   Article

   Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

   Insert

   CD44v3 (-cyt) (CD44 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   without cytoplasmic region, N280D in the V3 region

   Promoter

   PGK

   Available Since

   June 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPGK-T7/2-CD44v9 (-cyt)

   Plasmid

   #137819

   Purpose

   expression of CD44 proteins in mammalian cells

   Depositor

   Ursula Gunthert

   Article

   Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

   Insert

   CD44v9 (-cyt) (CD44 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   without cytoplasmic region

   Promoter

   PGK

   Available Since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET31b-T7-Spinach2-Pl-B

   Plasmid

   #79161

   Purpose

   Expresses the Pl-B biosensor under a T7 promoter

   Depositor

   Ming Hammond

   Article

   Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

   Insert

   T7-tRNA-Spinach2-Pl-B
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3 T7 Jip1(delta JBD)

   Plasmid

   #51767

   Purpose

   " JIP-1 may suppress signal transduction by the JNK pathway"

   Depositor

   Roger Davis

   Article

   Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

   Insert

   Jnk interacting protein 1 (Mapk8ip1 Mouse)
   

   Tags

   T7

   Expression

   Mammalian

   Mutation

   deleted 126-263

   Available Since

   March 14, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pCDNA3 T7 Jip4 (1-272)

   Plasmid

   #58928

   Purpose

   JIP4 serves as an activator of the p38 mitogen-activated protein (MAP) kinase pathway by a mechanism that requires the MAP kinase kinases MKK3 and MKK6

   Depositor

   Roger Davis

   Article

   Kelkar et al Mol Cell Biol. 2005 Apr;25(7):2733-43.

   Insert

   Mapk8ip4 (Spag9 Mouse)
   

   Tags

   T7

   Expression

   Mammalian

   Mutation

   Jip4 (1-272aa)

   Available Since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pCDNA3 T7 Jip4 (1-151)

   Plasmid

   #58926

   Purpose

   JIP4 serves as an activator of the p38 mitogen-activated protein (MAP) kinase pathway by a mechanism that requires the MAP kinase kinases MKK3 and MKK6

   Depositor

   Roger Davis

   Article

   Kelkar et al Mol Cell Biol. 2005 Apr;25(7):2733-43.

   Insert

   Mapk8ip4 (Spag9 Mouse)
   

   Tags

   T7

   Expression

   Mammalian

   Mutation

   Jip4 (1-151aa)

   Available Since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pCDNA3 T7 Jip4 (1-212)

    Plasmid

    #58927

    Purpose

    JIP4 serves as an activator of the p38 mitogen-activated protein (MAP) kinase pathway by a mechanism that requires the MAP kinase kinases MKK3 and MKK6

    Depositor

    Roger Davis

    Article

    Kelkar et al Mol Cell Biol. 2005 Apr;25(7):2733-43.

    Insert

    Mapk8ip4 (Spag9 Mouse)
    

    Tags

    T7

    Expression

    Mammalian

    Mutation

    Jip4 (1-212aa)

    Available Since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

11. T7-His-Arp11(1–417)

    Plasmid

    #51400

    Purpose

    expression of full-length mouse Arp11 under T7 promoter

    Depositor

    Trina Schroer

    Article

    Eckley et al Mol Biol Cell. 2003 Jul;14(7):2645-54. E

    Insert

    Arp11 (Actr10 Mouse)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pGGSG-Bromo-T7-P2a-Neo

    Plasmid

    #235516

    Purpose

    Cloning vector for GGSG-BromoTag-T7-P2A-Neo. Clone into ZraI and AfeI

    Depositor

    Brian Hendrich

    Insert

    BromoTag
    

    Use

    Mouse Targeting

    Tags

    BromoTag, T7, neo

    Mutation

    None

    Available Since

    May 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGGSG-Bromo-T7-T2a-Pac

    Plasmid

    #235518

    Purpose

    Cloning vector for GGSG-BromoTag-T7-T2A-Pac. Clone into PmlI and AfeI

    Depositor

    Brian Hendrich

    Insert

    BromoTag
    

    Use

    Mouse Targeting

    Tags

    BromoTag, T7, Puro

    Mutation

    None

    Available Since

    May 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGGSG-Bromo-T7-T2a-Bsd

    Plasmid

    #235519

    Purpose

    Cloning vector for GGSG-BromoTag-T7-T2A-Bsd. Clone into ZraI and AfeI

    Depositor

    Brian Hendrich

    Insert

    BromoTag
    

    Use

    Mouse Targeting

    Tags

    BromoTag, T7, Bsd

    Mutation

    None

    Available Since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGGSG-Bromo-T7-P2a-Pac

    Plasmid

    #235517

    Purpose

    Cloning vector for GGSG-BromoTag-T7-P2A-Pac. Clone into PmlI and AfeI

    Depositor

    Brian Hendrich

    Insert

    BromoTag
    

    Use

    Mouse Targeting

    Tags

    BromoTag, T7, Puro

    Mutation

    None

    Available Since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. T7-LacO-Halo-STReTCh-RGD

    Plasmid

    #216532

    Purpose

    Expresses HaloTag-STReTCh-RGD fusion protein in bacteria

    Depositor

    Alex Dunn

    Article

    Zhong et al Cell Rep Methods. 2022 Sep 2;2(9):100278

    Insert

    HaloTag-STReTCh-RGD
    

    Tags

    6xHis and HaloTag

    Expression

    Bacterial

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. L0-T7 Empty target Tp901

    Plasmid

    #195885

    Purpose

    Level 0 Empty Tp901 target: attP_Tp901-BpiI-attB_Tp901 with golden gate linker in pBLUNT plasmid.

    Depositor

    Jennifer Nemhauser

    Article

    Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

    Insert

    Empty Tp901 Target
    

    Use

    Synthetic Biology

    Available Since

    Sept. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. T7-TetO-QF_T7-LacO-TetR_p15A

    Plasmid

    #171659

    Purpose

    Expresses QF and/or TetR in BL21(DE3) E. coli according to the presence of aTc. Contains a p15A origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    T7-LacO-TetR-T7 Stop
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. QUAS-0-T7-TetO-GFP_ColE1

    Plasmid

    #171662

    Purpose

    QUAS directly upstream of a T7 promoter with a TetO operator. Expresses GFP in BL21(DE3) E. coli. Contains the ColE1 origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    QUAS-T7-TetO-GFP ssrA-T7 Stop
    

    Tags

    ssrA degradation tag (DAS+4)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET30-T7-lacO-His-sfGFP

    Plasmid

    #180754

    Purpose

    pET30 vector with sfGFP-S-tag inserted between the Bgl II and T7 terminator

    Depositor

    Michael Black

    Article

    Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

    Insert

    superfolder green fluorescent protein
    

    Tags

    strep tag

    Expression

    Bacterial

    Mutation

    G140C- Please see depositor comments

    Available Since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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