We narrowed to 24,452 results for: nes

1. pcDNA3-Venus-Venus-FLARE-AKAR

   Plasmid

   #123331

   Purpose

   Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-Venus-FLARE-AKAR
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, and Xpress…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-Cerulean3-Cerulean3-FLARE-EKAR-EV

   Plasmid

   #123342

   Purpose

   Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Cerulean3-Cerulean3-FLARE-EKAR-EV
   

   Use

   Tags

   6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. p2xFLAGhYAP2-WW1-mutant

   Plasmid

   #17795

   Depositor

   Marius Sudol

   Article

   Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

   Insert

   Yes-kinase associated protein (YAP1 Human)
   

   Use

   Tags

   2xFlag

   Expression

   Mammalian

   Mutation

   Tryptophan 199 to Alanine; Proline 202 to Alanine

   Promoter

   Available since

   May 9, 2008

   Availability

   Academic Institutions and Nonprofits only

4. p2xFLAGhYAP2-WW2-mutant

   Plasmid

   #17796

   Depositor

   Marius Sudol

   Article

   Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

   Insert

   Yes-kinase associated protein (YAP1 Human)
   

   Use

   Tags

   2xFlag

   Expression

   Mammalian

   Mutation

   Tryptophan 258 to Alanine Proline 261 to Alanine

   Promoter

   Available since

   May 9, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3-mCherry-mCherry-FLARE-EKAR-EV

   Plasmid

   #123341

   Purpose

   Red-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   mCherry-mCherry-FLARE-EKAR-EV
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pM-ErbB4-delta-kinase-PY3 mutant

   Plasmid

   #17802

   Depositor

   Marius Sudol

   Article

   Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

   Insert

   ErbB4 (ERBB4 Human)
   

   Use

   Tags

   GAL4-BD

   Expression

   Mammalian

   Mutation

   Carboxy-terminal fragment, amino acids 988-1292, …

   Promoter

   Available since

   May 9, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pLIC-His6-MBP-TEV-Rgs14

   Plasmid

   #236395

   Purpose

   Expresses His6-MBP-TEV-Rgs14 in E. coli

   Depositor

   John Hepler

   Article

   Brown et al J Biol Chem. 2015 Apr 3;290(14):9037-49.

   Insert

   Rattus norvegicus regulator of G-protein signaling 14 (Rgs14 Rat)
   

   Use

   Tags

   hexahistidine (H6) tag and maltose-binding protei…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. p40651-EFSNS-FMR1_e1ΔCGG-24xms2

   Plasmid

   #222969

   Purpose

   MS2 mRNA reporter with FMR1 5' UTR (CGG repeats removed) and exon 1 sequence

   Depositor

   Xinyu Zhao

   Article

   Sirois et al Cell Rep. 2024 Jun 11;43(6):114330. doi:

   Insert

   FMR1-ΔCGG 5' UTR and exon1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   31 CGG repeats deleted from 5' UTR

   Promoter

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiCRISPRv1-49535-sgFmr1_CGG5-2

   Plasmid

   #222964

   Purpose

   Targeting FMR1 5' UTR for CGG deletion

   Depositor

   Xinyu Zhao

   Article

   Sirois et al Cell Rep. 2024 Jun 11;43(6):114330. doi:

   Insert

   FMR1 sgRNA (FMR1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. T40A+Q54A

    Plasmid

    #217913

    Purpose

    A plasmid encoding the hydrogel-forming protein PXP with both the T40A and Q54A coil mutations

    Depositor

    Cole DeForest

    Article

    Bennett et al Adv Sci (Weinh). 2024 Mar 13:e2301708. d

    Insert

    PXP T40A+Q54A
    

    Use

    Tags

    6x His (N and C term)

    Expression

    Bacterial

    Mutation

    Threonine at position 41 and 253 mutated to Alani…

    Promoter

    T5

    Available since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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