Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 9,355 results for: APP

Showing: 4951 - 5000 of 9355 results

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H_NES 47kDa

Plasmid

#201348

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

HIV_NES-EGFP-3PrA

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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M_NLS 47kDa

Plasmid

#201343

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40A5-EGFP-3PrA

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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H_NLS 41kDa

Plasmid

#201340

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40-EGFP-2PrA

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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M_NES 41kDa

Plasmid

#201345

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

MAPK_NES-EGFP-2PrA

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-G2BRSCR

Plasmid

#185345

Purpose

Expression of soluble G2BR with scrambled sequence

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

scrambled gp78 G2BR sequence

Tags

EGFP

Expression

Mammalian

Mutation

scrambled sequence

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pTC3cIfm

Plasmid

#193778

Purpose

Expresses frame-shifted cI under the control of PLTetO-1 promoter, p15A origin of replication, Chloramphenicol selection

Depositor

Sangram Bagh

Article

Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

Insert

Frame-shifted CI

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLTetO-1

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsXRN1_1535-1706_K

Plasmid

#146782

Purpose

Mammalian Expression of HsXRN1_1535-1706

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

HsXRN1_1535-1706 (XRN1 Human)

Expression

Mammalian

Mutation

one non silent mutation S1688P compared to the se…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsDCP1b_H

Plasmid

#146505

Purpose

Mammalian Expression of HsDCP1b

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDCP1b (DCP1B Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsDCP2_G

Plasmid

#146401

Purpose

Mammalian Expression of HsDCP2

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDCP2 (DCP2 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmBruno_N

Plasmid

#147028

Purpose

Insect Expression of DmBruno

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmBruno (aret Fly)

Expression

Insect

Mutation

three non silent mutations S88T, H203R and A449R …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1-dsRNAres_N

Plasmid

#147029

Purpose

Insect Expression of DmNot1-dsRNAres

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmNot1-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmBruno_N

Plasmid

#147030

Purpose

Insect Expression of DmBruno

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmBruno (aret Fly)

Expression

Insect

Mutation

three non silent mutations S88T, H203R and A449R …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmHPat_57-968-dsRNAres_J

Plasmid

#146690

Purpose

Insect Expression of DmHPat_57-968-dsRNAres

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat_57-968-dsRNAres (Patr-1 Fly)

Expression

Insect

Mutation

one silent mutation A1758G and one non silent G56…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmHPat-del57-499-dsRNAres_J

Plasmid

#146691

Purpose

Insect Expression of DmHPat-del57-499-dsRNAres

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat-del57-499-dsRNAres (Patr-1 Fly)

Expression

Insect

Mutation

one silent mutation A1758G compared to the sequen…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmHPat_57-968-del500-684_J

Plasmid

#146695

Purpose

Insect Expression of DmHPat_57-968-del500-684

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat_57-968-del500-684 (Patr-1 Fly)

Expression

Insect

Mutation

two silent mutations compared to the sequence giv…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmMe31B-mut2_F

Plasmid

#146272

Purpose

Insect Expression of DmMe31B-mut2

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmMe31B-mut2 (me31B Fly)

Expression

Insect

Mutation

one silent mutation A1101G and one non silent mut…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmHPat_57-968-dsRNAres_H

Plasmid

#146464

Purpose

Insect Expression of DmHPat_57-968-dsRNAres

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat_57-968-dsRNAres (Patr-1 Fly)

Expression

Insect

Mutation

one silent mutation T477C and one non silent muta…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGO186N-KS1

Plasmid

#174301

Purpose

Replicating plasmid with sgRNA cassette containing a killing spacer #1 targeting the wild type polC gene.

Depositor

Lee Lynd

Article

Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

Insert

spacer expression cassette

Expression

Bacterial

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pDGO186N-KS2

Plasmid

#174302

Purpose

Replicating plasmid with sgRNA cassette containing a killing spacer #2 targeting the wild type polC gene.

Depositor

Lee Lynd

Article

Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

Insert

spacer expression cassette

Expression

Bacterial

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmGe1_J

Plasmid

#146688

Purpose

Insect Expression of DmGe1

Depositor

Elisa Izaurralde

Article

Behm-Ansmant et al Genes Dev. 2006 Jul 15. 20(14):1885-98.

Insert

DmGe1 (Ge-1 Fly)

Expression

Insect

Mutation

two non silent mutations Q194R and I1008T compare…

Available Since

Jan. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pOP004

Plasmid

#185985

Purpose

Encodes a 0.8 kb bacterial transcription unit with Broccoli-tRNA fluorogenic aptamer shortly upstream of the intrinsic terminator

Depositor

Georgi Belogurov

Article

Huang et al Methods Enzymol. 2022;675:207-233. doi:

Insert

Bacterial transcription unit encoding Broccoli-tRNA fluorogenic aptamer

Expression

Bacterial

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pETM-41P-HsXRN1_1174-1649-Strep_AH

Plasmid

#148901

Purpose

Bacterial Expression of HsXRN1_1174-1649

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2019 Sep 26;47(17):92

Insert

HsXRN1_1174-1649 (XRN1 Human)

Expression

Bacterial

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGO186N-KS3

Plasmid

#174303

Purpose

Replicating plasmid with sgRNA cassette containing a killing spacer #3 targeting the wild type polC gene.

Depositor

Lee Lynd

Article

Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

Insert

spacer expression cassette

Expression

Bacterial

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB-MeCP2_Hygro

Plasmid

#167899

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB-MeCP2

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB-MeCP2_EBFP2

Plasmid

#167897

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB-MeCP2

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only
pOP005

Plasmid

#186030

Purpose

Encodes a 0.8 kb bacterial transcription unit with Broccoli-tRNA fluorogenic aptamer shortly downstream of the promoter

Depositor

Georgi Belogurov

Article

Huang et al Methods Enzymol. 2022;675:207-233. doi:

Insert

Bacterial transcription unit encoding Broccoli-tRNA fluorogenic aptamer

Expression

Bacterial

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
NAX_CAGGS_CAS9_m4_KRAB-MeCP2_Puro

Plasmid

#167903

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB-MeCP2

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only
pENTR-ZmIAA29 (yeast codon optimized)

Plasmid

#188441

Purpose

Zea Mays Nuclear Auxin Signaling Repressor IAA29 with yeast codon optimization

Depositor

Britney Moss

Article

Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

Insert

Auxin Signaling Repressor IAA29 (yeast codon optimized)

Use

Cloning

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmDCP1-Y34A_P

Plasmid

#147214

Purpose

Insect Expression of DmDCP1-Y34A

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmDCP1-Y34A (Dcp1 Fly)

Expression

Insect

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NvG-DmDCP1_1-127_N

Plasmid

#147050

Purpose

Bacterial Expression of DmDCP1_1-127

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmDCP1_1-127 (Dcp1 Fly)

Expression

Bacterial

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSFDuetG-DmDCP1_328-366 (MCSII)-mut1_G

Plasmid

#146403

Purpose

Bacterial Expression of DmDCP1_328-366-mut1

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DmDCP1_328-366-mut1 (Dcp1 Fly)

Expression

Bacterial

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSFDuetG-DmDCP1_328-366 (MCSII)-mut2_G

Plasmid

#146404

Purpose

Bacterial Expression of DmDCP1_328-366-mut2

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DmDCP1_328-366-mut2 (Dcp1 Fly)

Expression

Bacterial

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSFDuetG-DmDCP1_328-366 (MCSII)-mut3_G

Plasmid

#146405

Purpose

Bacterial Expression of DmDCP1_328-366-mut3

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DmDCP1_328-366-mut3 (Dcp1 Fly)

Expression

Bacterial

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSFDuetG-DmDCP1_328-366 (MCSII)-WT_G

Plasmid

#146406

Purpose

Bacterial Expression of DmDCP1_328-366

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DmDCP1_328-366 (Dcp1 Fly)

Expression

Bacterial

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CBA-Con/Fon-EYFP-WPRE

Plasmid

#186752

Purpose

AAV-mediated Cre and Flp-dependent expression of EYFP under CBA promoter

Depositor

Yongling Zhu

Article

Xu et al Cell Rep. 2022 Jul 5;40(1):111036. doi:

Insert

EYFP

Use

AAV

Promoter

CBA

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmHPat-dsRNAres_J

Plasmid

#146692

Purpose

Insect Expression of DmHPat-dsRNAres

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat-dsRNAres (Patr-1 Fly)

Expression

Insect

Mutation

two non silent, A479V, G568D and one silent mutat…

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGB-Co-NF-kB

Plasmid

#180206

Purpose

Expression GAL4-fusion of Co-NF-kB in yeast

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

Co-NF-kB

Tags

GAL4 (1-147) fusion

Expression

Yeast

Mutation

aa 2-1224

Promoter

AdhI

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-HA-DmHPat-del500-684-dsRNAres_H

Plasmid

#146466

Purpose

Insect Expression of DmHPat-del500-684-dsRNAres

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat-del500-684-dsRNAres (Patr-1 Fly)

Expression

Insect

Mutation

two silent mutations compared to the sequence gi…

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1C-Fluc-par6_D

Plasmid

#146117

Purpose

Insect Expression of par6-3UTR

Depositor

Elisa Izaurralde

Article

Eulalio et al Genes Dev. 2007 Oct 15. 21(20):2558-70.

Insert

par6-3UTR (par-6 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmDCP1_1-127_A

Plasmid

#145901

Purpose

Insect Expression of DmDCP1_1-127

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmDCP1_1-127 (Dcp1 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmDCP1_128-372_A

Plasmid

#145902

Purpose

Insect Expression of DmDCP1_128-372

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmDCP1_128-372 (Dcp1 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmDCP1_1-127_A

Plasmid

#145903

Purpose

Insect Expression of DmDCP1_1-127

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmDCP1_1-127 (Dcp1 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmDCP1_128-372_A

Plasmid

#145904

Purpose

Insect Expression of DmDCP1_128-372

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmDCP1_128-372 (Dcp1 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFX-GalT-Sirius

Plasmid

#174469

Purpose

Express Sirius with golgi apparatus-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Sirius with golgi apparatus-targeting sequence

Tags

β1,4-galactose transferase

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB700 lenti gRNA EBFP2_TtoC and hairpin extension

Plasmid

#167921

Purpose

Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB700 lenti gRNA KanR neo_zhang2.0

Plasmid

#167917

Purpose

Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB700 lenti gRNA Blasto_TtoC and hairpin extension

Plasmid

#167920

Purpose

Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB700 lenti gRNA EYFP_TtoC and hairpin extension

Plasmid

#167922

Purpose

Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB700 lenti gRNA Hygro_TtoC and hairpin extension

Plasmid

#167923

Purpose

Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB700 lenti gRNA iRFP713_TtoC and hairpin extension

Plasmid

#167924

Purpose

Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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