Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 15,323 results for: pET

Clear keywords
Showing: 4951 - 5000 of 15323 results

  3. pUB-MSP1D1

    Plasmid
    #161609
    Purpose
    Bacterial expression of mutated membrane scaffold protein ("ultra-bright" MSP1D1) for Nanodisc formation
    Depositor
    Stephen Sligar
    Article
    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do
    Insert
    UD-MSP1D1
    Tags
    7-His tag
    Expression
    Bacterial
    Mutation
    mutant of MSP1D1 ( Q138W, L181W, Y236W)
    Promoter
    T7
    Available Since
    Nov. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    161609-plasmid-map-sequence-id-317666

    Enlarge

  5. pAGD289

    Plasmid
    #228113
    Purpose
    pET-21(+) deriviative with retron Kva1 containing a donor to edit E. coli rpoB gene cloned downstream of a T7 promoter
    Depositor
    Seth Shipman
    Article
    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103
    Insert
    Kva1 retron, ncRNA with 90 bp rpoB donor
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    228113-plasmid-map-sequence-id-455374

    Enlarge

  8. pCoofy48

    Plasmid
    #55186
    Purpose
    Bacterial expression vector for parallel SLIC cloning containing N-terminal 6x His and GST tags. C-terminal tags can be added during SLIC.
    Depositor
    Sabine Suppmann
    Article
    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.
    Type
    Empty backbone
    Tags
    6x His, GST, His10-Stop-OneStrep-Stop-S-tag-Stop-…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    55186-plasmid-map-sequence-id-464535

    Enlarge

  11. pHIT179

    Plasmid
    #204510
    Purpose
    Bacterial expression of CSR-1a with 6xHis and maltose binding protein tags for in vitro assays
    Depositor
    Yuji Kohara
    Article
    Aoki et al EMBO J. 2007 Dec 12. 26(24):5007-19.
    Insert
    csr-1a (C. elegans) (csr-1 Nematode)
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Promoter
    T7lac
    Available Since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    204510-plasmid-map-sequence-id-403822

    Enlarge

  13. pMH-macroHC

    Plasmid
    #82337
    Purpose
    Provides a C-terminal macro domain and 6xHis tag for expression in E. coli
    Depositor
    Michael Hothorn
    Article
    Wild et al Protein Sci. 2016 Oct 24. doi: 10.1002/p
    Insert
    SPX domain of the Vacuolar Transporter Chaperone 4 (VTC4 Budding Yeast)
    Tags
    6xHis non-cleavable and macro domain of human his…
    Expression
    Bacterial
    Mutation
    Truncated protein, amino acids 1-178
    Available Since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    82337-plasmid-map-sequence-id-155830

    Enlarge

  16. pPD3C

    Plasmid
    #86971
    Purpose
    Expresses PCNA3 protein fused phosphite dehydrogenase and P450cam in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038
    Insert
    PCNA3 fused to phosphite dehydrogenase and P450cam
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    E175A/A176R in PTDH, N106R in PCNA3, silent mutat…
    Promoter
    T7 promoter
    Available Since
    March 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    86971-plasmid-map-sequence-id-173928

    Enlarge

  20. EGF-A-SpyTag

    Plasmid
    #246944
    Purpose
    Expresses multifunctional construct for YES-gated release of Epidermal Growth Factor (EGF) from materials in response to sortase eSrtA(2A9) treatment. Contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Article
    Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199
    Insert
    Please see depositor comments below
    Tags
    6x His tag
    Expression
    Bacterial
    Available Since
    Nov. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    246944-plasmid-map-sequence-id-489549

    Enlarge

  21. bla-A OR T-SpyTag

    Plasmid
    #246945
    Purpose
    Expresses multifunctional construct for OR-gated release of beta-lactamase (bla) from materials in response to sortase eSrtA(2A9) OR TEV treatment. Contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Article
    Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199
    Insert
    Please see depositor comments below
    Tags
    6x His tag
    Expression
    Bacterial
    Available Since
    Nov. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    246945-plasmid-map-sequence-id-489550

    Enlarge

  22. mCherry-S-SpyTag

    Plasmid
    #243786
    Purpose
    Expresses multifunctional construct enabling the YES-gated release of mCherry from materials in response to sortase eSrtA(4S9) treatment. Construct contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Article
    Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/
    Insert
    mCherry
    Tags
    6x His tag
    Expression
    Bacterial
    Available Since
    Oct. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    243786-plasmid-map-sequence-id-486522

    Enlarge

  31. MZB164

    Plasmid
    #217842
    Purpose
    10xHis-SUMO (Smt3) fused, HRV-3C cleavable, Halotag for rapid cloning/DNA assembly of pulldown targets via SwaI restriction site for E. coli based protein expression
    Depositor
    Richard Baker
    Article
    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    HaloTag
    Tags
    10x His, HaloTag, and SUMO (SMT3)
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    217842-plasmid-map-sequence-id-433000

    Enlarge

  47. pPD1cR

    Plasmid
    #86970
    Purpose
    Expresses PCNA1 variant (G108C) fused phosphite dehydrogenase and putidaredoxin reductase in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038
    Insert
    PCNA1 variant (G108C) fused to phosphite dehydrogenase and putidaredoxin reductase
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    E175A/A176R in PTDH, G108C in PCNA1
    Promoter
    T7 promoter
    Available Since
    March 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    86970-plasmid-map-sequence-id-173927

    Enlarge

  48. pPD1R

    Plasmid
    #86969
    Purpose
    Expresses PCNA1 protein fused phosphite dehydrogenase and putidaredoxin reductase in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038
    Insert
    PCNA1 fused to phosphite dehydrogenase and putidaredoxin reductase
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    E175A/A176R in PTDH
    Promoter
    T7 promoter
    Available Since
    March 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    86969-plasmid-map-sequence-id-173876

    Enlarge

  49. pPD2cX

    Plasmid
    #86968
    Purpose
    Expresses PCNA2 variant (L171C) fused phosphite dehydrogenase and putidaredoxin in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038
    Insert
    PCNA2 variant (L171C) fused to phosphite dehydrogenase and putidaredoxin
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    E175A/A176R in PTDH, C73S/C85S in PdX, L171C in P…
    Promoter
    T7 promoter
    Available Since
    March 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    86968-plasmid-map-sequence-id-173869

    Enlarge

Showing: 4951 - 5000 of 15323 results