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pYNL-N1_3xNLS

Plasmid

#65672

Purpose

Expresses NLS-YNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

three copies of SV40 nuclear localization signal

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pFRT_TO_FLAGHA_PRKRA_sgRNA_PAM_mutant

Plasmid

#106112

Purpose

Inducible gene expression of FlagHA-PRKRA sgRNA PAM mutant

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

PRKRA (PRKRA Human)

Tags

Flag and HA

Expression

Mammalian

Mutation

silent mutation of Gly32 (GGG-->GGT)

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pYNL-N1_Mito

Plasmid

#65669

Purpose

Expresses Mito-YNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

cytochrome c oxidase subunit VIII

Use

Luciferase

Tags

YNL

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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GTT-ir

Plasmid

#81112

Purpose

Gene Tagging vector TRP/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Insert

tdiRFP

Use

Cre/Lox

Tags

tdiRFP

Expression

Yeast

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pONL-N1_zyxin

Plasmid

#65683

Purpose

Expresses Zyxin-ONL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

zyxin (Zyx Mouse)

Use

Luciferase

Tags

ONL

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2015

Availability

Academic Institutions and Nonprofits only
pONL-N1_3xNLS

Plasmid

#65674

Purpose

Expresses NLS-ONL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

three copies of SV40 nuclear localization signal

Use

Luciferase

Tags

ONL

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pONL-N1_TUBB5

Plasmid

#65680

Purpose

Expresses beta-tubulin-ONL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

beta-tubulin class V

Use

Luciferase

Tags

ONL

Expression

Mammalian

Promoter

CMV

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA4/HisMaxB-YAP2-deltaC

Plasmid

#21124

Depositor

Marius Sudol

Article

Tsutomu Oka & Marius et al

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

His

Expression

Mammalian

Mutation

5 Carboxy-terminal amino acids -FLTWL are deleted

Available Since

July 1, 2009

Availability

Academic Institutions and Nonprofits only
MIGR1-IFNLR1-TEV-SV40-hygro

Plasmid

#254112

Purpose

Retroviral transfer vector for IFNLR1-TEV fusion gene expression, Hygromycin selection marker, for CyCLoPs platform.

Depositor

Jun Huh

Article

Lu et al Cell. 2026 Feb 5;189(3):939-955.e26. doi

Insert

IFNLR1-TEV

Use

Retroviral

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pFA6-CFAPoptim-NAT

Plasmid

#221129

Purpose

Plasmid for generating integration cassette to C-terminally tag gene of interest with FAPoptim::NATMX

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

FAPoptim

Expression

Yeast

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-SEC61-FAPoptim

Plasmid

#221126

Purpose

FAP-tagged marker for the ER (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

SEC61-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-RPA34-FAPoptim

Plasmid

#221123

Purpose

FAP-tagged marker for the nucleus (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

RPA34-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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3xMS-TR-100-PURO HRD

Plasmid

#207582

Purpose

homologous recombination donor for the insertion of the 3xMS2-tag at the endogenous locus

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

3xMS2-TR and 100 bp of downstream sequence followed by a PuroR and flanked by endogenous TR sequences

Tags

3xMS2

Expression

Mammalian

Promoter

SV40

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGFP-UUG-IRES-mCherry

Plasmid

#222112

Purpose

Start codon reporter with mutated start codon

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

GFP-IRES-mCherry

Use

Lentiviral

Mutation

UUG start codon

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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Pt NaV1.4

Plasmid

#200233

Purpose

Expression in mammalian cells or oocytes

Depositor

Dan Minor

Article

Abderemane-Ali et al J Gen Physiol. 2021 Sep 6;153(9):e202112

Insert

Phyllobates terriblis NaV1.4 – muscle channel

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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Dt NaV1.4

Plasmid

#200250

Purpose

Expression in mammalian cells or oocytes

Depositor

Dan Minor

Article

Abderemane-Ali et al J Gen Physiol. 2021 Sep 6;153(9):e202112

Insert

Dendrobates tinctorius NaV1.4 – muscle channel

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-ExSYTE.H64A

Plasmid

#216262

Purpose

inactive ExSYTE.H64A (P2A) EGFP with 3'UTR of Rat Arc DTE under synapsin promoter

Depositor

Susumu Tomita

Article

Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

Insert

ExSYTE P2A EGFP - Rat DTE

Use

AAV

Mutation

H64A mutation in each oTetR

Available Since

May 8, 2024

Availability

Academic Institutions and Nonprofits only

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NFAST(low)-Halo-ER

Plasmid

#214419

Purpose

ER localization of low-affinity NFAST fused to Halo-ER

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspA-N

Tags

Halo

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pL0_AC-CaMK2

Plasmid

#202112

Purpose

Level 0 plasmid containing the promoter from gene 39236 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

CaMK2 promoter

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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CMV EGFP-PPM1H-H153D

Plasmid

#200432

Purpose

Mammalian expression of H153D mutation in PPM1H

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

PPM1H protein phosphatase, Mg2+/Mn2+ dependent 1H

Tags

EGFP

Expression

Mammalian

Mutation

H153D

Promoter

CMV

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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