We narrowed to 75,765 results for: Rest
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Plasmid#104580PurposeContains mouse IgG2c CH1/CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Full Fc-mediated immune effector functionsDepositorInsertMouse IgG2c CH1/CH2/CH3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
HLA_DRB1_07_01
Plasmid#165014PurposeExpression of HLA-DRB1*07:01 cDNADepositorInsertHLA-DRB1*07:01 (HLA-DRB1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
TTVTFLPTPSLILQT_epitope
Plasmid#165021PurposeExpression of CMV UL44 (38-52) fused to CD74DepositorInsertCMV UL44 (38-52)
UseLentiviralTagsCD74 at N-term; cathepsin S cleavage site (GRWHTV…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Akt2
Plasmid#86593PurposeExpresses eGFP-tagged human Akt2(WT)DepositorInsertAkt2 (AKT2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-Akt2(WT)
Plasmid#86623PurposeExpresses mCherry-tagged human Akt2(WT)DepositorInsertAkt2 (AKT2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+-Tornado-split-nLuc
Plasmid#212612PurposeFor the expression of a split nLuc that produces luminescence only when circularized. To test your IRES of interest, clone into the EcoRI, BsiWI sites.DepositorInsertTornado-CVB3-split-nLuc
UseLuciferaseTagsExpressionMammalianMutationC373T mutation in CVB3PromoterCMVAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
C1-MPAct-mCherry
Plasmid#155222PurposeMonitor changes in the density of membrane-proximal F-actin (MPA)DepositorInsertF-tractin (aa 9-52 of rat ITPKA) fused to mCherry C-term tagged with the CaaX sequence derived from Kras4b
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
mc2 mNeon
Plasmid#206218PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
HLA_DRB1_04_01
Plasmid#165013PurposeExpression of HLA-DRB1*04:01 cDNADepositorInsertHLA-DRB1*04:01 (HLA-DRB1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRV2-GG
Plasmid#105349Purposevirus-induced gene silencingDepositorInsertTRV-RNA2(5')
UseIn planta gene silencing; viralTagsExpressionMutationBsaI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
mc2 mCherry hPGK
Plasmid#206231PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
C1-MPAct-mRuby3
Plasmid#155221PurposeMonitor changes in the density of membrane-proximal F-actin (MPA)DepositorInsertF-tractin (aa 9-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b
UseTagsmRuby3ExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
C1-MPAct-mCitrine
Plasmid#155220PurposeMonitor changes in the density of membrane-proximal F-actin (MPA)DepositorInsertF-tractin (aa 9-52 of rat ITPKA) fused to mCitrine C-term tagged with the CaaX sequence derived from Kras4b
UseTagsmCitrineExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-2
Plasmid#160702Purposegpdh promoter from Trichoderma virens bordered by BsaI restriction sitesDepositorInsertgpdh
UseSynthetic BiologyTagsExpressionMutationIncorporation of BsaI restriction sitesPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
HLA_DRA_01_01
Plasmid#165012PurposeExpression of HLA-DRA*01:01 cDNADepositorInsertHLA-DRA (HLA-DRA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-MPAct-mRuby3-IRES-Puro
Plasmid#155231Purposelentiviral MPAct constructDepositorInsertLentiviral expression of MPAct-mRuby sensor
UseLentiviralTagsmRuby3ExpressionMutationPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
2HA-pDEST-C
Plasmid#118374PurposeGateway compatible mammalian expression vector with C-terminal 2HA to tag proteins of interest.DepositorTypeEmpty backboneUseDestinationTags2HAExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2 Neo
Plasmid#127644PurposeEmpty pLentiCRISPRv2 with NeoRDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBRα-β flap (831-1057)
Plasmid#53734PurposeExpression of alpha NTD fused via three alanines to E. coli RNAP subunit beta, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.DepositorInsertalpha NTD-Beta flap fusion
UseTagsExpressionBacterialMutationPromoterlpp/lacUV5 (tandem promoter)Available sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTEI039
Plasmid#105428PurposeSynthetic biologyDepositorInsertLexA-VP16-ER (transcription factor: inducible expression; estradiol)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only