We narrowed to 5,326 results for: pcdna3.1

1. pcDNA3.1-Nmyc-Eps8L2_1-496-siR

   Plasmid

   #196515

   Purpose

   Expression of Myc-Eps8L2_1-496

   Depositor

   Edgar Gomes

   Article

   Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

   Insert

   Eps8L2_1-496 (EPS8L2 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Silent mutations for siRNA resistance

   Promoter

   T7 promoter

   Available Since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)-Cue1pTM-MYC-UBE2G2-FLAG

   Plasmid

   #185347

   Purpose

   Membrane anchoring of UBE2G2 and co-IP. Encodes transmembrane domain of S. Cerevisiae Cue1p fused to MYC-UBE2G2 with C-terminal FLAG tag

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   Cue1p/Ube2G2 fusion (internal MYC tag)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Feb. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)-myc-OGA(GS-544)C181_NES

   Plasmid

   #193998

   Purpose

   plasmid encoding myc-tagged OGA(GS-544)C181_NES

   Depositor

   Christina Woo

   Article

   Ge et al ACS Chem Biol. 2023 Jan 4. doi: 10.1021/

   Insert

   myc-OGA(GS-544)C181_NES
   

   Tags

   myc

   Expression

   Mammalian

   Available Since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-p15 Bid-KKAA-GFP

   Plasmid

   #191970

   Purpose

   Expression of the active p15 cBid KKAA mutant, chimera with GFP.

   Depositor

   Luca Scorrano

   Insert

   p15 - cBid KKAA
   

   Expression

   Mammalian

   Mutation

   KKAA: Lys 157 and Lys 158 into Ala

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+)-DnaJB1(H33Q)-PKAcat-mCherry

   Plasmid

   #181854

   Purpose

   mCherry-tagged DnaJB1-PKA catalytic subunit fusion with H33Q DnaJB1 mutation to block Hsp70 binding; for mammalian expression.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Histidine 33 in DnaJB1 changed to glutamine.

   Promoter

   CMV

   Available Since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-MCS-BirA(R118G)-HA-TARG1

   Plasmid

   #172583

   Purpose

   For transient expression of C-terminal BirA-tagged TARG1

   Depositor

   Karla Feijs

   Article

   Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

   Insert

   TARG1 (OARD1 Human)
   

   Tags

   BirA(R118G)-HA

   Expression

   Mammalian

   Available Since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-MCS-BirA(R118G)-HA-MACROD2

   Plasmid

   #172582

   Purpose

   For transient expression of C-terminal BirA-tagged MACROD2

   Depositor

   Karla Feijs

   Article

   Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

   Insert

   MACROD2 (MACROD2 Human)
   

   Tags

   BirA(R118G)-HA

   Expression

   Mammalian

   Available Since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-MS2-HA-HsNanos3-del4-20_X

   Plasmid

   #147945

   Purpose

   Mammalian Expression of HsNanos3-del4-20

   Depositor

   Elisa Izaurralde

   Article

   Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

   Insert

   HsNanos3-del4-20 (NANOS3 Human)
   

   Expression

   Mammalian

   Available Since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-MS2-HA-HsRck_296-472-10xmut_AC

   Plasmid

   #148420

   Purpose

   Mammalian Expression of HsRck_296-472-10xmut

   Depositor

   Elisa Izaurralde

   Article

   Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

   Insert

   HsRck_296-472-10xmut (DDX6 Human)
   

   Expression

   Mammalian

   Mutation

   Q309A, H312A, T316A, R320A, Q322A, N324A, R335A, …

   Available Since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-kappa-myc-dK-2xG4S-TMst

    Plasmid

    #145766

    Purpose

    Expresses myc-dL5(E52K, L91S) low affinity Fluorogen Activating Protein (FAP) on the extracellular surface anchored to the mammalian cell plasma membrane with the PDGFR transmembrane domain.

    Depositor

    Marcel Bruchez

    Article

    Carpenter et al ACS Chem Biol. 2020 Aug 17. doi: 10.1021

    Insert

    kappa-myc-dK-2xG4S-TMst
    

    Expression

    Mammalian

    Mutation

    The dK FAP is dL5 with mutations at E50K, L89S, a…

    Promoter

    CMV

    Available Since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1 LIC 6A CARD8-FLAG F405G

    Plasmid

    #169956

    Purpose

    Mammalian expression of CARD8-FLAG with a site III mutation (F405G)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    F405G

    Promoter

    CMV

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-2xFLAG-2xSTREP-CCND1(P287S)

    Plasmid

    #172644

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged cyclin D1(P287S) in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Pro287Ser

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-2xFLAG-2xSTREP-CCND1(T286I)

    Plasmid

    #172645

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged cyclin D1(T286I) in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Thr286Ile

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-2xFLAG-2xSTREP-CCND1(P287L)

    Plasmid

    #172646

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged cyclin D1(P287L) in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Pro287Leu

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 LIC 6A CARD8-FLAG R394E

    Plasmid

    #169954

    Purpose

    Mammalian expression of CARD8-FLAG with a site III mutation (R394E)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    R394E

    Promoter

    CMV

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG

    Plasmid

    #169958

    Purpose

    Mammalian expression of the CARD8 C-terminus fused to ubiquitin (co-translationally processed)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Tags

    FLAG and Ub

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1 LIC 6A CARD8-FLAG L368G

    Plasmid

    #169952

    Purpose

    Mammalian expression of CARD8-FLAG with a site III mutation (L368G)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    L368G

    Promoter

    CMV

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1 LIC 6A CARD8-FLAG E274R

    Plasmid

    #169950

    Purpose

    Mammalian expression of CARD8-FLAG that cannot bind DPP9 due to a mutation at site I (E274R)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E274R

    Promoter

    CMV

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 LIC 6A CARD8-FLAG F370G

    Plasmid

    #169953

    Purpose

    Mammalian expression of CARD8-FLAG with a site III mutation (F370G)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    F370G

    Promoter

    CMV

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-N-Myc_DW164AA + DED/AAA USP7

    Plasmid

    #131256

    Purpose

    Mammalian expression of N-terminally Myc-tagged USP7, with mutations in the TRAF-like domain (DW164AA) and UBL2 (DE758AA_D764A)

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Contains point mutations that convert USP7 aspart…

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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