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We narrowed to 8,358 results for: BLI;

Showing: 4041 - 4080 of 8358 results

  1. p159-RV-syn-dio-mScarlet-T2A

    Plasmid
    #185700
    Purpose
    Retroviral vector expression cre-dependent membrane tagged mScarlet-T2A driven by the rat Synapsin promoter (with XhoI and PacI at 3'end of T2A to clone a gene of interest)
    Depositor
    Lynette Lim
    Insert
    mScarlet
    Use
    Retroviral
    Tags
    mScarlet palmitoylation with T2A on 3'-end
    Expression
    Mutation
    Promoter
    Synapsin
    Available since
    July 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    185700-plasmid-map-sequence-id-366334

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  2. pGEMHE-TNKS-2-M1054V

    Plasmid
    #138538
    Purpose
    For making TNKS-2-M1054V RNA
    Depositor
    Carmen Williams
    Insert
    Tankyrase 2 (TNKS2 Human)
    Use
    In vitro transcription
    Tags
    FLAG
    Expression
    Mutation
    Changed methionine 1054 to valine
    Promoter
    T7
    Available since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    138538-plasmid-map-sequence-id-291736

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  3. pAAV-CaMKIIa-hM4D(Gi)-mCherry-WPRE-pA(Short)

    Plasmid
    #183704
    Purpose
    AAV to express hM4D(Gi)-mCherry fusion protein. Viral genome 3' UTR modified to bring the viral genome below the optimal AAV packaging limit
    Depositor
    Jonathan Ploski
    Insert
    hM4D(Gi)
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    CaMKIIa
    Available since
    May 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    183704-plasmid-map-sequence-id-360830

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  4. pAAV-CaMKIIa-hM3D(Gq)-mCherry-WPRE-pA(Short)

    Plasmid
    #183705
    Purpose
    AAV to express hM3D(Gq)-mCherry fusion protein. Viral genome 3' UTR modified to bring the viral genome below the optimal AAV packaging limit
    Depositor
    Jonathan Ploski
    Insert
    hM3D(Gq)
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    CaMKIIa
    Available since
    May 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183705-plasmid-map-sequence-id-360768

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  5. loxP-T2A-Myr-Casp8-ERT2-rox

    Plasmid
    #182362
    Purpose
    Shuttle vector for T2A-Myr-Casp8-ERT2
    Depositor
    Mario Capecchi
    Insert
    loxP-T2A-Myr-Casp8-ERT2-rox
    Use
    Cre/Lox and Mouse Targeting; Dre / rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182362-plasmid-map-sequence-id-358219

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  6. loxP-T2A-Myr-DeadCasp8-ERT2-rox

    Plasmid
    #182361
    Purpose
    Shuttle vector for T2A-Myr-DeadCasp8-ERT2
    Depositor
    Mario Capecchi
    Insert
    loxP-T2A-Myr-DeadCasp8-ERT2-rox
    Use
    Cre/Lox and Mouse Targeting; Dre / rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182361-plasmid-map-sequence-id-358216

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  7. pMSP1D12W/2Y

    Plasmid
    #124275
    Purpose
    Study of protein insertion into nanodisks by means of changes in tryptophan fluorescence
    Depositor
    Alexey S. Ladokhin
    Insert
    Membrane scaffold protein
    Use
    Tags
    7xHis-tag
    Expression
    Bacterial
    Mutation
    Two tryptophans changed to two tyrosines
    Promoter
    T7
    Available since
    March 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    124275-plasmid-map-sequence-id-242402

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  8. pCDZ2FE_Rev3_1873-2025

    Plasmid
    #105672
    Purpose
    Expresses Flag-tagged fragment (amino acids 1873-2025) of human Rev3 protein
    Depositor
    Thanos Halazonetis
    Insert
    Rev3L (REV3L Human)
    Use
    Tags
    2X Flag tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    105672-plasmid-map-sequence-id-203200

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  9. pcDNA3.1-EOLA1/CXORF40A

    Plasmid
    #180776
    Purpose
    Expresses EOLA1/CXORF40A in mammalian cells
    Depositor
    Douglas Feldman
    Insert
    EOLA1 (EOLA1 Human)
    Use
    Tags
    SBP-Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    180776-plasmid-map-sequence-id-356523

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  10. SDCBPA-c020

    Plasmid
    #175484
    Purpose
    Protein expression in bacterial cells. PDZ1 and PDZ2 domains, G111-V288.
    Depositor
    Opher Gileadi
    Insert
    SDCBP (SDCBP Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175484-plasmid-map-sequence-id-353208

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  11. STX4AA-c005

    Plasmid
    #175487
    Purpose
    Protein expression in bacterial cells. Coiled-coil domains, M1-L275.
    Depositor
    Opher Gileadi
    Insert
    STX4 (RUNX1T1 Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175487-plasmid-map-sequence-id-353181

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  12. CAG-loxP-3xpA-loxP-sfGFP+HsbG syntron

    Plasmid
    #172477
    Purpose
    sfGFP with a synthetic intron derived from the human beta-globin gene
    Depositor
    Mario Capecchi
    Insert
    sfGFP
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    172477-plasmid-map-sequence-id-338915

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  13. CAG-loxP-3xpA-loxP-sfGFP+pCI syntron

    Plasmid
    #172478
    Purpose
    sfGFP with a synthetic intron from the pCI expression vector (Promega)
    Depositor
    Mario Capecchi
    Insert
    sfGFP
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    172478-plasmid-map-sequence-id-338912

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  14. CAG-loxP-3xpA-loxP-sfGFP+MmIghe syntron

    Plasmid
    #172475
    Purpose
    sfGFP with a synthetic intron derived from the mouse Ighe gene
    Depositor
    Mario Capecchi
    Insert
    sfGFP
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    172475-plasmid-map-sequence-id-338910

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  15. CAG-loxP-3xpA-loxP-sfGFP+RbG syntron

    Plasmid
    #172476
    Purpose
    sfGFP with a synthetic intron derived from the rabbit beta-globin gene
    Depositor
    Mario Capecchi
    Insert
    sfGFP
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    172476-plasmid-map-sequence-id-338906

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  16. pRNATU6.1 HDAC3 shRNA1

    Plasmid
    #112249
    Purpose
    HDAC3 KD
    Depositor
    Simone Di Giovanni
    Insert
    shRNA against mouse HDAC3 (Hdac3 Mouse)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    112249-plasmid-map-sequence-id-213639

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  17. pANY4-pR18/pL18-ccdB

    Plasmid
    #164899
    Purpose
    Two valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pR18/pL18-ccdB.
    Depositor
    Yingfeng An
    Insert
    ccdB cassette
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    164899-plasmid-map-sequence-id-327990

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  18. pLL1271

    Plasmid
    #129282
    Purpose
    Expression plasmid with pLL1270 as backbone and alpha-Xylp(Ga0256695_ 1211)
    Depositor
    Lee Lynd
    Insert
    alpha-Xylp_1211 (Ga0256695_ 1211)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Clostridium thermocellum DSM1313 Cellobiose Phosp…
    Available since
    March 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    129282-plasmid-map-sequence-id-260608

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  19. KSP_HNL

    Plasmid
    #129772
    Purpose
    Expresses Xenopus Kinesin-5 KSP motor and neck linker domain monomer for biochemical assays
    Depositor
    William Hancock
    Insert
    KSP (kif11.L Frog)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Truncation at 367
    Promoter
    Available since
    Dec. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    129772-plasmid-map-sequence-id-264857

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  20. Unc104_K560GFP

    Plasmid
    #129773
    Purpose
    Expresses worm Kinesin-3 Unc104 head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP tagged
    Depositor
    William Hancock
    Insert
    Unc104 (unc-104 Nematode)
    Use
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    Truncation at 361, fused to kinesin-1 starting Al…
    Promoter
    Available since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129773-plasmid-map-sequence-id-263568

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  21. pcDNA-mTRAF3ip3∆TM-CFP

    Plasmid
    #125182
    Purpose
    Expresses mouse TRAF3IP3 ∆TM in mammalian cells
    Depositor
    Shao-Cong Sun
    Insert
    TRAF3ip3 (Traf3ip3 Mouse)
    Use
    Tags
    CFP
    Expression
    Mammalian
    Mutation
    K87R and S359P- See depositor comments below
    Promoter
    CMV
    Available since
    May 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    125182-plasmid-map-sequence-id-243310

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  22. lentiCRISPRv2_PROX1_1+4

    Plasmid
    #121177
    Purpose
    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.
    Depositor
    Timo Otonkoski
    Insert
    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    Use
    CRISPR and Lentiviral
    Tags
    P2A-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    121177-plasmid-map-sequence-id-237896

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  23. LentiCRISPR_Hs_MBD3_ex2

    Plasmid
    #62623
    Purpose
    Encodes gRNA which targets human MBD3
    Depositor
    Paul Wade
    Insert
    MBD3 gRNA (MBD3 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EFS
    Available since
    Nov. 30, 2018
    Availability
    Academic Institutions and Nonprofits only

  24. LentiCRISPR_Hs_MBD3_ex3

    Plasmid
    #62624
    Purpose
    Encodes gRNA which targets human MBD3
    Depositor
    Paul Wade
    Insert
    MBD3 gRNA (MBD3 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EFS
    Available since
    Nov. 30, 2018
    Availability
    Academic Institutions and Nonprofits only

  25. pmSod1-Q42L;Q123A-N’His

    Plasmid
    #117710
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    Sod1 (Sod1 Mouse)
    Use
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Q42L; Q123A
    Promoter
    T7
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117710-plasmid-map-sequence-id-227524

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  26. pHH0103 HYPB WW domain

    Plasmid
    #104267
    Purpose
    Bacterial expression of WW domain from HYPB
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    HYPB WW domain (SETD2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104267-plasmid-map-sequence-id-203491

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  27. pBCSK_LEU2_II

    Plasmid
    #109061
    Purpose
    yeast shuttle vector for insertion into yeast chromosomes in pBCSK backbone. LoxP sites downstream of LEU2. For teaching purposes.
    Depositor
    Colin Logie
    Insert
    LEU2 (LEU2 Budding Yeast, Zygosaccharomyces rouxii)
    Use
    Cre/Lox
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    LEU2
    Available since
    Aug. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    109061-plasmid-map-sequence-id-209544

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  28. pACYCduet-mxLOX2-EH

    Plasmid
    #104979
    Purpose
    Biosynthesis of oxylipins by microbial enzymes
    Depositor
    Deokkun Oh
    Inserts
    MXAN_1744 (MXAN_1744 Myxococcus xanthus)
    MXAN_1644 (MXAN_1644 Myxococcus xanthus)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    May 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    104979-plasmid-map-sequence-id-207891

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  29. pHH0103 WWOX WW domain #2

    Plasmid
    #104236
    Purpose
    Bacterial expression of WW domain #2 from WWOX
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    WWOX WW domain #2 (WWOX Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Feb. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    104236-plasmid-map-sequence-id-204371

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  30. Plekhm1 C279 pGEX 4T2-GST

    Plasmid
    #73835
    Purpose
    Express human Plekhm1 (C279 to end domain) in bacterial cells
    Depositor
    Paul Odgren
    Insert
    Plekhm1 (PLEKHM1 Human)
    Use
    Tags
    Gst
    Expression
    Bacterial
    Mutation
    to vector was cloned last 279 aa from human Plek…
    Promoter
    Available since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only

  31. Plekhm1 -PH1/2 pGEX 4T2-GST

    Plasmid
    #73831
    Purpose
    Express human Plekhm1 (PH1 and PH2 domains) in bacterial cells
    Depositor
    Paul Odgren
    Insert
    Plekhm1 (PLEKHM1 Human)
    Use
    Tags
    Gst
    Expression
    Bacterial
    Mutation
    to vector was cloned Ph1 and Ph2 domains of human…
    Promoter
    Available since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only

  32. pJK611

    Plasmid
    #72532
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ); large subunit (AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 and n/a
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    72532-plasmid-map-sequence-id-132620

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  33. pJK626

    Plasmid
    #72461
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Cys/Tyr154>Phe mutant (AaPurE1-S57C,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutation changes serine-57 to cysteine, tyrosine-…
    Promoter
    T7
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72461-plasmid-map-sequence-id-133899

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  34. pJK691

    Plasmid
    #72381
    Purpose
    Produces Escherichia coli BolA, N-terminal His6 tag (H6EcBolA)
    Depositor
    T. J. Kappock
    Insert
    stationary-phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC (bolA Escherichia coli str. K-12 substr. MG1655)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72381-plasmid-map-sequence-id-134006

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  35. pJK292

    Plasmid
    #73371
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/His89>Asn mutant (AaPurE1-H59N,H89N)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changed histidine-59 to asparagine and histidine-…
    Promoter
    T7
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73371-plasmid-map-sequence-id-135107

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  36. pJK293

    Plasmid
    #73372
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Phe/His89>Phe mutant (AaPurE1-H59F,H89F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changed histidine-59 to phenylalanine and histidi…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    73372-plasmid-map-sequence-id-135108

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  37. pJK294

    Plasmid
    #73373
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asp/His89>Asp mutant (AaPurE1-H59D,H89D)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changed histidine-59 to aspartate and histidine-8…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    73373-plasmid-map-sequence-id-135109

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  38. pJK528

    Plasmid
    #71625
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Met105>Ser/Ile109>Trp mutant (TdPurE2-M105S,I109W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes methionine-105 to serine, isoleucine-109 …
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71625-plasmid-map-sequence-id-131668

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  39. pJK439

    Plasmid
    #72478
    Purpose
    Produces Thermoplasma acidophilum citrate synthase pseudo-dimer
    Depositor
    T. J. Kappock
    Insert
    citrate synthase (artificial dimer)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    inserted NSTCLVPRGSPV sequence between C- and N-t…
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72478-plasmid-map-sequence-id-134617

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  40. pJK404

    Plasmid
    #72417
    Purpose
    Produces Acetobacter aceti 1023 Est12, N-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    acyl-CoA thioesterase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    additional N-terminal 15 residues MSETYGTGQKEQHC,…
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72417-plasmid-map-sequence-id-135656

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Showing: 4041 - 4080 of 8358 results