We narrowed to 18,831 results for: Tec

1. ABE-RA4.0

   Plasmid

   #208305

   Purpose

   Expresses ABE-RA4.0 in mammalian cells

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   TadA-RA4.0-SpCas9 D10A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   P48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…

   Promoter

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-PRLSS-yATOM_SH2-KDEL

   Plasmid

   #209714

   Purpose

   Mammalian expression of y-ATOM biosensor for SH2 localized to endoplasmic reticulum

   Depositor

   Stewart Loh

   Article

   Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

   Insert

   PRLSS-yATOM_SH2-KDEL
   

   Use

   Tags

   PRLSS

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-MBP-yATOM_WDR5 (R80A)

   Plasmid

   #209713

   Purpose

   Mammalian expression of y-ATOM biosensor for WDR5 with binding null R80A mutation

   Depositor

   Stewart Loh

   Article

   Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

   Insert

   MBP-yATOM_WDR5 (R80A)
   

   Use

   Tags

   MBP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-PRLSS-NbGFP_miRFP670nano3-KDEL

   Plasmid

   #209718

   Purpose

   Mammalian expression of NbGFP-miRFP670nano3 localized to endoplasmic reticulum

   Depositor

   Stewart Loh

   Article

   Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

   Insert

   PRLSS-NbGFP_miRFP670nano3-KDEL
   

   Use

   Tags

   PRLSS

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-PRLSS-Clover-KDEL

   Plasmid

   #209720

   Purpose

   Mammalian expression of clover localized to endoplasmic reticulum

   Depositor

   Stewart Loh

   Article

   Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

   Insert

   PRLSS-Clover-KDEL
   

   Use

   Tags

   PRLSS

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-Cox4-yATOM_SH2

   Plasmid

   #209715

   Purpose

   Mammalian expression of y-ATOM biosensor for SH2 localized to mitochondria

   Depositor

   Stewart Loh

   Article

   Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

   Insert

   Cox4-yATOM_SH2
   

   Use

   Tags

   Cos4

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PlayBack-CMV-Bamh1

   Plasmid

   #203303

   Purpose

   For expression of eukaryotic genes of interest with CMV promoter with Bamh1 as a PlayBack Restriction Enzyme

   Depositor

   Aleksandar Necakov

   Article

   Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PlayBack-CMV-Nhe1

   Plasmid

   #203304

   Purpose

   For expression of eukaryotic genes of interest with CMV promoter with NheI as a PlayBack Restriction Enzyme

   Depositor

   Aleksandar Necakov

   Article

   Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PlayBack-CMV-Bgl11

   Plasmid

   #203306

   Purpose

   For expression of eukaryotic genes of interest with CMV promoter with BglII as a PlayBack Restriction Enzyme

   Depositor

   Aleksandar Necakov

   Article

   Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PlayBack-Ef1a-Nde1

    Plasmid

    #203309

    Purpose

    For expression of eukaryotic genes of interest with Ef1a promoter with NdeI as a PlayBack Restriction Enzyme

    Depositor

    Aleksandar Necakov

    Article

    Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PlayBack-Ef1a-Nhe1

    Plasmid

    #203310

    Purpose

    For expression of eukaryotic genes of interest with Ef1a promoter with NheI as a PlayBack Restriction Enzyme

    Depositor

    Aleksandar Necakov

    Article

    Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMXSIP-Rab5B-HA

    Plasmid

    #204709

    Purpose

    expresses Rab5B-HA in mammalian cells

    Depositor

    William Kaelin

    Article

    Wyant et al Science. 2022 Aug 5;377(6606):621-629. d

    Insert

    RAB5B (RAB5B Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMXSIP-TMEM141-FLAG

    Plasmid

    #204702

    Purpose

    expresses TMEM141-FLAG in mammalian cells

    Depositor

    William Kaelin

    Article

    Wyant et al Science. 2022 Aug 5;377(6606):621-629. d

    Insert

    TMEM141 (TMEM141 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GB3682

    Plasmid

    #187807

    Purpose

    Transcriptional unit for expression of alcohol O-acetyltransferase from Saccharomyces pastorianus strain CBS 1483 chromosome SeVIII-SeXV, codon optimized for Nicotiana.

    Depositor

    Diego Orzaez

    Article

    Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

    Insert

    SpATF1-2
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBZS-YP_009701874

    Plasmid

    #198378

    Purpose

    Express YP_009701874 in bacterial cells.

    Depositor

    Takashi Ito

    Article

    Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

    Insert

    YP_009701874 (CVA-1_543R )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBZS-YP_001426001

    Plasmid

    #198361

    Purpose

    Express pBZS-YP_001426001 in bacterial cells.

    Depositor

    Takashi Ito

    Article

    Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

    Insert

    YP_001426001 (N369L )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBZS-YP_009665506

    Plasmid

    #198376

    Purpose

    Express YP_009665506 in bacterial cells.

    Depositor

    Takashi Ito

    Article

    Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

    Insert

    YP_009665506 (NYs-1_727R )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBZS-YP_009665369

    Plasmid

    #198375

    Purpose

    Express YP_009665369 in bacterial cells.

    Depositor

    Takashi Ito

    Article

    Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

    Insert

    YP_009665369 (NYs-1_421L )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTA2EGFP

    Plasmid

    #193756

    Purpose

    Expresses EGFP under the control of PLTetO-1 promoter, ColE1 origin of replication, Ampicillin selection

    Depositor

    Sangram Bagh

    Article

    Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLTetO-1

    Available Since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTC3RBSC1cI

    Plasmid

    #193773

    Purpose

    Expresses lambda CI under the control of PLTetO-1 promoter, p15A origin of replication, Chloramphenicol selection

    Depositor

    Sangram Bagh

    Article

    Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

    Insert

    Lambda CI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLTetO-1

    Available Since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pX*C3E2-Crimson

    Plasmid

    #193754

    Purpose

    Expresses E2-Crimson under the control of PLUX* promoter, p15A origin of replication, Chloramphenicol selection

    Depositor

    Sangram Bagh

    Article

    Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

    Insert

    E2-Crimson
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLUX*

    Available Since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PRC3MKO2

    Plasmid

    #193757

    Purpose

    Expresses mKO2 under the control of PR promoter, p15A origin of replication, Chloramphenicol selection

    Depositor

    Sangram Bagh

    Article

    Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

    Insert

    mKO2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PR

    Available Since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLC3RBSC2cI

    Plasmid

    #193775

    Purpose

    Expresses lambda CI under the control of PLLacO-1 promoter, p15A origin of replication, Chloramphenicol selection

    Depositor

    Sangram Bagh

    Article

    Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

    Insert

    Lambda CI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLLacO-1

    Available Since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJA2LUXR(F)

    Plasmid

    #193755

    Purpose

    Expresses LuxR in forward direction under the control of BBa_J23102 constitutive promoter, ColE1 origin of replication, Ampicillin selection

    Depositor

    Sangram Bagh

    Article

    Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

    Insert

    LuxR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23102

    Available Since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGWT35S-KOC207-MXMT

    Plasmid

    #182730

    Purpose

    Transient expression of KOC207-MXMT in plant cell

    Depositor

    Yutaka Kodama

    Article

    Fujii et al Biotechniques. 2018 Apr;64(4):153-161. d

    Insert

    KOC207
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S promoter

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDA313

    Plasmid

    #195214

    Purpose

    Expression of superfolder GFP (sfGFP) by a hybrid T7 promoter with tetO.

    Depositor

    Rahul Sarpeshkar

    Article

    Deng et al Front Bioeng Biotechnol. 2022 Sep 28;10:

    Insert

    sfGFP with tetO and a hairpin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pPB UbC Arrestin-uTEV1-V5; UAS-mCherry

    Plasmid

    #173122

    Purpose

    Expresses uTEV1 and reporter receiver construct in DIPG

    Depositor

    Alice Ting

    Article

    Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

    Insert

    Arrestin-uTEV1-V5; UAS-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAG CAG CCL20-pre-mGRASP

    Plasmid

    #173114

    Purpose

    Expresses CCL20 sender construct in mammalian cells

    Depositor

    Alice Ting

    Article

    Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

    Insert

    CCL20-pre-mGRASP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCDNA3 CMV myc-Arrestin-TEVp

    Plasmid

    #173115

    Purpose

    Expresses arrestin receiver construct in mammalian cells

    Depositor

    Alice Ting

    Article

    Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

    Insert

    myc-Arrestin-TEVp
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pPB UbC Arrestin-TEVp(WT)-V5; UAS-mCherry

    Plasmid

    #173121

    Purpose

    Expresses TEVp(WT) and reporter receiver construct in DIPG

    Depositor

    Alice Ting

    Article

    Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

    Insert

    Arrestin-TEVp(WT)-V5; UAS-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. LLP722_pSP15

    Plasmid

    #192435

    Purpose

    To test the relative expression strength of the TCTP1 promoter

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    TCTP1::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. LLP720_pSP13

    Plasmid

    #192433

    Purpose

    To test the relative expression strength of the 35S promoter

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    35S::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. LLP721_pSP14

    Plasmid

    #192434

    Purpose

    To test the relative expression strength of the Act2 promoter

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. p09008_AF1-4+GFP5-7

    Plasmid

    #173715

    Purpose

    Plasmid backbone for the HyperXpress workflow containing new to nature hybrid GFP with strong fluorescence in E. coli cell free protein synthesis extracts.

    Depositor

    Johannes Kabisch

    Article

    Zibulski et al Front Bioeng Biotechnol. 2022 Apr 1;10:8

    Inserts

    hybrid GFP
    mKate2
    

    Use

    E. coli cell free protein synthesis extracts

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pYTK001_E2At(2nd)

    Plasmid

    #183085

    Purpose

    2A peptide E2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assembly

    Depositor

    Zhen Wang

    Article

    Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

    Insert

    E2A
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pYTK001_O2At(2nd)

    Plasmid

    #183086

    Purpose

    2A peptide O2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assembly

    Depositor

    Zhen Wang

    Article

    Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

    Insert

    O2A
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pYTK001_P2At(2nd)

    Plasmid

    #183087

    Purpose

    2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assembly

    Depositor

    Zhen Wang

    Article

    Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

    Insert

    P2A
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. L0-AA008

    Plasmid

    #185754

    Purpose

    Level 0 loop assembly vector containing the Anthoceros agrestis EF1a promoter (pAaEF1a).

    Depositor

    Peter Szoevenyi

    Article

    Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph

    Insert

    pAaEF1a (promoter)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. L0-AA018

    Plasmid

    #185753

    Purpose

    Level 0 loop assembly vector containing the Anthoceros agrestis Tip1;1 promoter (pAaTip1;1).

    Depositor

    Peter Szoevenyi

    Article

    Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph

    Insert

    pAaTip1;1 (promoter)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET30-T7-lacO-His-sfGFP

    Plasmid

    #180754

    Purpose

    pET30 vector with sfGFP-S-tag inserted between the Bgl II and T7 terminator

    Depositor

    Michael Black

    Article

    Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

    Insert

    superfolder green fluorescent protein
    

    Use

    Tags

    strep tag

    Expression

    Bacterial

    Mutation

    G140C- Please see depositor comments

    Promoter

    Available Since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge