We narrowed to 44,084 results for: cha

1. YIplac204-TDH3p-CDC19-CYC1t

   Plasmid

   #216122

   Purpose

   Yeast integrative vector for constitutive expression of CDC19 at the TRP1 locus

   Depositor

   Yvonne Nygård

   Article

   Choi et al Metab Eng. 2024 Jul;84:23-33. doi: 10.10

   Insert

   Pyruvate kinase 1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH3 promoter

   Available Since

   Dec. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAM72

   Plasmid

   #227622

   Purpose

   pETDuet-1 Rpn8(1-179)-precission-Strep, H6-precission-Rpn11(2-239, G77P)

   Depositor

   Andreas Martin

   Article

   Worden et al Mol Cell. 2017 Sep 7;67(5):799-811.e8. d

   Inserts

   Rpn11 (RPN11 Budding Yeast)
   rpn8 (RPN8 Budding Yeast)
   

   Use

   Tags

   His-Prescission

   Expression

   Bacterial

   Mutation

   1-179 and aa 2-239, G77P

   Promoter

   T7

   Available Since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pYAMTr2GCsgSeLEU2

   Plasmid

   #224870

   Purpose

   Disruption of S. eubayanus type LEU2 genes

   Depositor

   Katsunori Suzuki

   Article

   Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

   Insert

   pYAMTr2GC having the guide sequence SeLEU2 (DI49_0736 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pYAMTrGCsgScLEU2

   Plasmid

   #224869

   Purpose

   Disruption of S. cerevisiae type LEU2 genes

   Depositor

   Katsunori Suzuki

   Article

   Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

   Insert

   pYAMTrGCas having the guide sequence ScLEU2 (LEU2 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBWB507

   Plasmid

   #209325

   Purpose

   p15A-tetR-tet-ME-RBS-mRFP-ME-U1-Barcode-U2-dbI terminator-oriT-Cm. Plasmid backbone for fragment expression.

   Depositor

   Adam Arkin

   Article

   Biggs et al Mol Syst Biol. 2024 Oct 7. doi: 10.1038/

   Insert

   mRFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tet

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pYAMTr2GCsgScLEU2

   Plasmid

   #224868

   Purpose

   Disruption of S. cerevisiae type LEU2 gene

   Depositor

   Katsunori Suzuki

   Article

   Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

   Insert

   pYAMTr2GC having the guide sequence ScLEU2 (LEU2 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAG426-GAL-APP-CTF

   Plasmid

   #181732

   Purpose

   Galactose inducible expression of APP-CTF

   Depositor

   Alejandro Chavez

   Insert

   APP-CTF (APP Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   C-terminal fragment (aa 671-770)

   Promoter

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. ClhN-pADH3-GFP-Pex3-4V5-APEX2-URA

   Plasmid

   #207064

   Purpose

   Expressing GFP-Pex3-4V5-APEX2 under ADH3 promoter. Insufficient signal for EM.

   Depositor

   Zhiping Xie

   Article

   Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

   Insert

   Pex3
   

   Use

   Tags

   4xV5-APEX2 and GFP

   Expression

   Yeast

   Mutation

   Promoter

   pADH3

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. BcC _pET29b

   Plasmid

   #215403

   Purpose

   Expression construct for BcC gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   BcC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pITESceIcmd

    Plasmid

    #218288

    Purpose

    Introduction of a cyanamide-inducible I-SceI expression cassette, use in combination with pITEdv1

    Depositor

    Claudia Vickers

    Article

    McDonnell et al bioRxiv 2024.03.26.586389

    Insert

    HO(-253, -1)-tSynth3

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. OoH_pET29b

    Plasmid

    #215424

    Purpose

    Expression construct for OoH gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    OoH
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Ph-NylC_pET28a

    Plasmid

    #215425

    Purpose

    Expression construct for Ph-NylC gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    Ph-NylC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ReU_pET29b

    Plasmid

    #215426

    Purpose

    Expression construct for ReU gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    ReU
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ThcCut2 _pET29b

    Plasmid

    #215430

    Purpose

    Expression construct for ThcCut2 gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    ThcCut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TtA_pET29b

    Plasmid

    #215432

    Purpose

    Expression construct for TtA gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    TtA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MsmegA _pET29b

    Plasmid

    #215411

    Purpose

    Expression construct for MsmegA gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    MsmegA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Nc-NylC_pET28a

    Plasmid

    #215412

    Purpose

    Expression construct for Nc-NylC gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    Nc-NylC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AoC_pET29b

    Plasmid

    #215402

    Purpose

    Expression construct for AoC gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    AoC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. M-NylC_pET28a

    Plasmid

    #215409

    Purpose

    Expression construct for M-NylC gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    M-NylC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ClhN-pADH3-Snf7-4V5-APEX2-URA

    Plasmid

    #207065

    Purpose

    Moderate overexpression of Snf7-4V5-APEX2 under ADH3 promoter. Marker for late endosomes. Only partially functional.

    Depositor

    Zhiping Xie

    Article

    Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

    Insert

    Snf7
    

    Use

    Tags

    4xV5-APEX2

    Expression

    Yeast

    Mutation

    Promoter

    pADH3

    Available Since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. TagBFP2-Mss4D636K-Kina

    Plasmid

    #202725

    Purpose

    expresses fluorescent protein-tagged enzyme

    Depositor

    Gerry Hammond

    Article

    Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

    Insert

    mTagBFP2:S. cerevisiae Mss4(377-756)(D636K) (MSS4 Budding Yeast)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEM.T02R

    Plasmid

    #198654

    Purpose

    Easy-MISE toolkit pEM-plasmid containing CYC1 terminator with IL protruding ends

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    tCYC1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEM.T01L

    Plasmid

    #198653

    Purpose

    Easy-MISE toolkit pEM-plasmid containing ADH1 terminator with BC protruding ends

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    tADH1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEM.P06R

    Plasmid

    #198652

    Purpose

    Easy-MISE toolkit pEM-plasmid containing PDA1 promoter with FG protruding ends

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    pPDA1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEM.P06L

    Plasmid

    #198651

    Purpose

    Easy-MISE toolkit pEM-plasmid containing PDA1 promoter with EF protruding ends

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    pPDA1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEM.P05R

    Plasmid

    #198650

    Purpose

    Easy-MISE toolkit pEM-plasmid containing CYC1 promoter with FG protruding ends

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    pCYC1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEM.P05L

    Plasmid

    #198649

    Purpose

    Easy-MISE toolkit pEM-plasmid containing CYC1 promoter with EF protruding ends

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    pCYC1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEM.P04R

    Plasmid

    #198648

    Purpose

    Easy-MISE toolkit pEM-plasmid containing TPI1 promoter with FG protruding ends

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    pTPI1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEM.P04L

    Plasmid

    #198647

    Purpose

    Easy-MISE toolkit pEM-plasmid containing TPI1 promoter with EF protruding ends

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    pTPI1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEM.P03R

    Plasmid

    #198646

    Purpose

    Easy-MISE toolkit pEM-plasmid containing PGK1 promoter with FG protruding ends

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    pPGK1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pEM.P03L

    Plasmid

    #198645

    Purpose

    Easy-MISE toolkit pEM-plasmid containing PGK1 promoter with EF protruding ends

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    pPGK1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pEM.P02R

    Plasmid

    #198644

    Purpose

    Easy-MISE toolkit pEM-plasmid containing ENO2 promoter with FG protruding ends

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    pENO2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pEM.P02L

    Plasmid

    #198643

    Purpose

    Easy-MISE toolkit pEM-plasmid containing ENO2 promoter with EF protruding ends

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    pENO2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pEM.P01R

    Plasmid

    #198642

    Purpose

    Easy-MISE toolkit pEM-plasmid containing TDH3 promoter with FG protruding ends

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    pTDH3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pEM.P01L

    Plasmid

    #198641

    Purpose

    Easy-MISE toolkit pEM-plasmid containing TDH3 promoter with EF protruding ends

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    pTDH3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pOBD2

    Plasmid

    #196826

    Purpose

    Yeast two-hybrid ; Gal4 DNA binding domain

    Depositor

    Stan Fields

    Article

    Uetz et al Nature. 2000 Feb 10;403(6770):623-7. doi

    Type

    Empty backbone

    Use

    Tags

    Gal4 DNA binding domain

    Expression

    Yeast

    Mutation

    Promoter

    ADH

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGEX-6P-1-Cys-HA-Ubiquitin

    Plasmid

    #185356

    Purpose

    In vitro ubiquitination assays

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    Ubiquitin
    

    Use

    Tags

    GST, HA(x2)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. LT2_32_PyruEth_X2site

    Plasmid

    #194428

    Purpose

    Plasmid for genome integration in X2 site expressing PyruEth (Pyruvate and Ethanol consumption sensors)

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

    Insert

    PyruEth
    

    Use

    Used as donor dna in genome integration after lin…

    Tags

    Expression

    Yeast

    Mutation

    NA

    Promoter

    Available Since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. LT1_75_Donor_Tag_RPL13A-mTurq2

    Plasmid

    #194415

    Purpose

    Plasmid for genome integration of mTurquoise2 CDS to tag native RPL13A for biosensors RibPro and UPRpro

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

    Insert

    mTurquoise2 CDS
    

    Use

    Synthetic Biology; Used as donor dna in genome in…

    Tags

    Expression

    Yeast

    Mutation

    NA

    Promoter

    Available Since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pnYC-NpM-SpDcp2opt_1-242-E147Q_AD

    Plasmid

    #148546

    Purpose

    Bacterial Expression of SpDcp2opt_1-242-E147Q

    Depositor

    Elisa Izaurralde

    Article

    Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

    Insert

    SpDcp2opt_1-242-E147Q (dcp2 Fission Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pILGFP4D42

    Plasmid

    #185840

    Purpose

    Testing the TEF1 promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1-PTEF1+[Z268]>yEGFP>TPGK1-TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pILGFP10CF71

    Plasmid

    #185860

    Purpose

    Testing the SkGAL2 promoter inserted with 4 Z268 elements using a green fluorescent protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1- PSkGAL2+[Z268]>(BamHI)yEGFP>TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pILGFP10CF72 (B9)

    Plasmid

    #185858

    Purpose

    Testing the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1- PSkGAL2M5(5*PZ4)>(BamHI)yEGFP>TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pILGFP10CF72

    Plasmid

    #185857

    Purpose

    Testing the SkGAL2 promoter inserted with 4 PZ4 elements using a green fluorescent protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1- PSkGAL2M4(4*PZ4)>(BamHI)yEGFP>TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pILGFP10E1B

    Plasmid

    #185856

    Purpose

    Testing the SkGAL2 promoter inserted with 3 PZ4 elements using a green fluorescent protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1- PSkGAL2M2(3*PZ4)>(BamHI)yEGFP>TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pILGFP10E1A

    Plasmid

    #185855

    Purpose

    Testing the SkGAL2 promoter inserted with 2 PZ4 elements using a green fluorescent protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1- PSkGAL2M1(2*PZ4)> (BamHI)yEGFP>TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pILGFP3A2 (1)

    Plasmid

    #185851

    Purpose

    Testing the TEF1 promoter appended with 2 tetracycline riboswitch using a green fluorescence protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1-PTEF1>2*TcRb>yEGFP>TPGK1-TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. KBB441

    Plasmid

    #185131

    Purpose

    Swi6 full length with L->A mutated in nuclear localization signal

    Depositor

    Ken Belanger

    Article

    Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

    Insert

    SWI6
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    SWI6 deletion of amino acids 450-458

    Promoter

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pCfB9341 (pgRNA_IX-1_NatMX)

    Plasmid

    #161593

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site IX-1

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Insert

    guiding RNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pCfB9343 (pgRNA_XV-1_NatMX)

    Plasmid

    #161595

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XV-1

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Insert

    guiding RNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge