We narrowed to 4,126 results for: Alk

1. pCAG-mScarlet-Giantin

   Plasmid

   #196872

   Purpose

   Expresion of Giantin-fused to mScarlet. Used as Golgi marker

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   mScarlet-Giantin (GOLGB1 Synthetic, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Quimeric CAG

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNK2657

   Plasmid

   #219741

   Purpose

   MoClo-compatible Level 0 promoterless vector encoding hispidin-synthase from Mycena citricolor codon-optimised for expression in Nicotiana benthamiana, Pichia pastoris, Homo sapiens

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   hispidin- synthase from Mycena citricolor
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNK115

   Plasmid

   #219745

   Purpose

   MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 codon-optimised for expression in Nicotiana benthamiana

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   mutant of fungal luciferase
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mutation

   I3S, N4T, F11L, I63T, T99P, T192S, A199P

   Promoter

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNK116

   Plasmid

   #219742

   Purpose

   MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Nicotiana benthamiana

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   mutant of fungal hispidin-3-hydroxylase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   D37E, V181I, S323M, M385K

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. R619-M63-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2

   Plasmid

   #166010

   Purpose

   mammalian expression of SARS-CoV-2 soluble spike trimer protein

   Depositor

   Dominic Esposito

   Article

   Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

   Insert

   SARS-CoV-2 S (spike) (S )
   

   Use

   Tags

   3C-His8-Strep2x2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV51

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. R619-M64-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2 D614G

   Plasmid

   #166011

   Purpose

   mammalian expression of SARS-CoV-2 soluble spike trimer protein (D614G mutant)

   Depositor

   Dominic Esposito

   Article

   Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

   Insert

   SARS-CoV-2 S (spike) (S )
   

   Use

   Tags

   3C-His8-Strep2x2

   Expression

   Mammalian

   Mutation

   D614G

   Promoter

   CMV51

   Available since

   May 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBabe 3XFLAG-wtGATA6-3XAU1 puro

   Plasmid

   #72607

   Purpose

   Expresses an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human wtGATA6 in mamalian cells

   Depositor

   Kevin Janes

   Article

   Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

   Insert

   GATA binding protein 6 (GATA6 Human)
   

   Use

   Retroviral

   Tags

   3XAU1 and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-TRE-V5-TurboID-T2A-SARS-CoV2-NSP7-bGH polyA > mPGK-BlastR-SV40 polyA

   Plasmid

   #218914

   Purpose

   PiggyBac transposition

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Insert

   V5-TurboID-T2A-SARS-CoV2-NSP7/BlastR (ORF1ab )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   2363G>C and 4330T>C

   Promoter

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEN_TT 3XFLAG-wtGATA6-3XAU1

   Plasmid

   #72612

   Purpose

   Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged human wtGATA6

   Depositor

   Kevin Janes

   Article

   Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

   Insert

   GATA binding protein 6 (GATA6 Human)
   

   Use

   Entry vector for gateway cloning

   Tags

   3XAU1 and 3XFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   TRE tight promoter

   Available since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. LCV2_LacZ_sgRNA_2

    Plasmid

    #155093

    Purpose

    lentiviral plasmid expressing Cas9 and gRNA targeting LacZ

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    LacZ_sgRNA_2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPB-TRE-V5-TurboID-linker-T2A-GR-bGH polyA > mPGK-BlastR-SV40 polyA

    Plasmid

    #218910

    Purpose

    PiggyBac transposition

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    V5-TurboID-T2A-huGR/BlastR (NR3C1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    2363G>C and 4155C>T and 4729T>G and 4731…

    Promoter

    Available since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBactin-mOrange2-NEDD1-gTBD

    Plasmid

    #196858

    Purpose

    Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mOrange2-N-gTBD (NEDD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLRC1-NEK10p:NEK10-3XFLAG

    Plasmid

    #137030

    Purpose

    Lentiviral vector for expressing NEK10 under the control of the endogenous NEK10 promoter

    Depositor

    David Sabatini

    Article

    Chivukula et al Nat Med. 2020 Jan 20. pii: 10.1038/s4159

    Insert

    NIMA-like kinase 10 (NEK10 Human)
    

    Use

    Lentiviral

    Tags

    3XFLAG

    Expression

    Mutation

    Promoter

    human NEK10 endogenous promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRetroX-mCRT-GPI-IRES-DsRed

    Plasmid

    #223711

    Purpose

    Mammalian expression of mouse CRT-GPI

    Depositor

    Judy Lieberman

    Article

    Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

    Insert

    Calreticulin-GPI (Calr Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-CRT-Fc FL

    Plasmid

    #221853

    Purpose

    Express human Calreticulin (full-length) with C-terminal human IgG1Fc tag in mammalian cell.

    Depositor

    Judy Lieberman

    Article

    Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

    Insert

    calreticulin (CALR Human)
    

    Use

    Tags

    Human IgG1Fc tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBactin-EGFP-NEDD1-gTBD

    Plasmid

    #196857

    Purpose

    Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to enhanced (E) GFP. Used to displace endogenous gamma-TuRC from the centrosome.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    EGFP-N-gTBD (NEDD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUB6-HuMGF

    Plasmid

    #190782

    Purpose

    Expresses membrane-bound form of human SCF in mammalian cells

    Depositor

    Jakob Reiser

    Article

    Zhang et al Retrovirology. 2010 Jan 25;7:3. doi: 10.

    Insert

    human stem cell factor (KITLG Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. EphA2 430-976 pcDNA3.1

    Plasmid

    #102738

    Purpose

    Expression of c terminal EphA2 in mammalian cells

    Depositor

    Jin Chen

    Article

    Amato et al Cancer Res. 2016 Jan 15;76(2):305-18. do

    Insert

    EphA2 N terminal (430-976aa) (Epha2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. BE3-NLS-NLS-P2A-BLAST

    Plasmid

    #135348

    Purpose

    Expression of BE3 (Base editor generation three) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    BE3-2XNLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET51b-Strep-hAGT-His

    Plasmid

    #167276

    Purpose

    Overexpression of the human o_-alkylguanine-dna alkyltransferase in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    hAGT (AGT Human)
    

    Use

    Tags

    His x10 and Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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