We narrowed to 4,276 results for: GCA

1. pTX198

   Plasmid

   #89262

   Purpose

   Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsPDS gene, OsPDS-gRNA01

   Depositor

   Daniel Voytas

   Article

   Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

   Insert

   OsPDS-gRNA01
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Plant

   Mutation

   Promoter

   Maize Ubiquitin1 promoter

   Available since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-hU6-mU6-Cre2GO

   Plasmid

   #136907

   Purpose

   Lentivirus for base editing activatable Cre recombinase expression in mammalian cells. All in one vector with sgCre2GO.

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   BlasCyGO
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   ATG>ACG

   Promoter

   SFFV

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV hUbC-Cas9-P2A-Puro_NFATC2-sgRNA

   Plasmid

   #188704

   Purpose

   Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and 4 sgRNAs targeting human NFATC2

   Depositor

   David Tobin

   Article

   Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

   Insert

   NFATC2 sgRNAs
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. CROP_C9_Puro_GART

   Plasmid

   #183296

   Purpose

   All-in-One CRISPRko system with a guide RNA that targets GART gene

   Depositor

   Prashant Mali

   Article

   Hu et al Biomaterials. 2022 Jan;280:121276. doi:

   Insert

   GART
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBSU6-shTim23/CMV-eGFP

   Plasmid

   #89795

   Purpose

   Expresses shRNA against TIM23 with a separate promoter for expression of GFP.

   Depositor

   Robert Friedlander

   Article

   Yano et al Nat Neurosci. 2014 Jun;17(6):822-31. doi

   Insert

   shRNA Tim23 (Timm23 Mouse)
   

   Use

   Tags

   eGFP under separate promoter

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pColE1_sgRNA_M7NS_AmpR

   Plasmid

   #119157

   Purpose

   Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with seven mismatches in the non-seed region

   Depositor

   Vincent Noireaux

   Article

   Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

   Insert

   sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with seven mismatches in the non-seed region
   

   Use

   Crispr

   Tags

   Expression

   Mutation

   Promoter

   J23119

   Available since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOC5 Tubb3-GFP KI

   Plasmid

   #183440

   Purpose

   FlpOFF knock-in for beta3-tubulin GFP (amino acid position: stop codon)

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   gRNA and GFP donor
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pABM-D4-miR30-CypA

   Plasmid

   #132934

   Purpose

   Lentivector for CypA knockdown and blasticidin resistance

   Depositor

   Jeremy Luban

   Article

   Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

   Insert

   CypA shRNA
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTorPE-G-GECO1.1

   Plasmid

   #32467

   Depositor

   Robert Campbell

   Article

   Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

   Insert

   G-GECO1.1
   

   Use

   Lab constructed

   Tags

   6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

   Expression

   Bacterial

   Mutation

   GCaMP3 K69E/N77Y/D86G/K119I/L173Q/K380N/S404G/E43…

   Promoter

   Available since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-Flag-Tpr-Si

    Plasmid

    #60918

    Purpose

    Expresses Flag tagged nucleoporin Tpr resistant to siRNA GCACAACAGGATAAGGTTA

    Depositor

    Vinay Nandicoori

    Article

    Rajanala et al PLoS One. 2012;7(1):e29921. doi: 10.1371

    Insert

    TPR (TPR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Silent point mutations in siRNA target region; E1…

    Promoter

    CMV

    Available since

    Jan. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. double_sgRNA targeting e3 and e7

    Plasmid

    #190689

    Purpose

    sgRNAs targeting enhancer 3 and 7 of MYC separately

    Depositor

    Stanley Qi

    Article

    Lin et al Science. 2022 Aug 11:eabk3512. doi: 10.1

    Insert

    sgRNAs targeting enhancer 3 and 7 of MYC separately
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    BFP and Puromycin

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. CROP_C9_Puro_FGFR1

    Plasmid

    #183289

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets FGFR1 gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    FGFR1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSR9.1347

    Plasmid

    #39540

    Depositor

    Katharina Strub

    Article

    Mary et al RNA. 2010 May;16(5):969-79. Epub 2010 Ma

    Insert

    SRP9 (SRP9 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pORANGE Tubb3-4xGFP KI

    Plasmid

    #183445

    Purpose

    GFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and 4xGFP donor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Geph UTR shRNA

    Plasmid

    #121070

    Purpose

    shRNA targeting the 3'-untranslated region (UTR) of the gephyrin mRNA

    Depositor

    Angel de Blas

    Article

    Yu et al Mol Cell Neurosci. 2007 Dec;36(4):484-50

    Insert

    GPHN shRNA (Gphn Rat)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. CROP_C9_Puro_ESR1

    Plasmid

    #183288

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets ESR1 gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    ESR1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSR-puro-Sec5

    Plasmid

    #31117

    Depositor

    Christopher Counter

    Article

    Issaq et al Mol Cancer Res. 2010 Feb . 8(2):223-31.

    Insert

    Sec5 shRNA (EXOC2 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pSuper.Retro.Puro-PKN1 shRNA 4

    Plasmid

    #59978

    Purpose

    Knockdown of PKN1

    Depositor

    Debu Chakravarti

    Article

    Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

    Insert

    PKN1 shRNA
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

19. MS2_adRNA

    Plasmid

    #124624

    Purpose

    Expresses MS2_adRNA targeting the RAB7A transcript along with GFP

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

    Insert

    MS2_adRNA_RAB7A
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBbdCas9S_Psyn-sgRNAmreB

    Plasmid

    #149653

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi mreB

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAmreB
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, Psyn

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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