We narrowed to 29,405 results for: des.2

1. pLV-mScarlet-SARS-CoV-2-5'UTR

   Plasmid

   #184638

   Purpose

   Fluorescent reporter for mammalian cells: Expression of mScarlet with SARS-CoV-2 5'UTR

   Depositor

   Hao Wu

   Article

   Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

   Insert

   mScarlet
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 2, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pET21-pelB-LaG-41-4GS-LaG-2

   Plasmid

   #172766

   Purpose

   Bacterial expression of dimerized anti-GFP nanobodies LaG-41 and LaG-2 (4GS linker)

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-41-4GS-LaG-2
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSb_init containing the TM287/288 nanobody Nb_TM#2

   Plasmid

   #132698

   Purpose

   expresses the TM287/288 nanobody Nb_TM#2 in E.coli, to use as positive control in ELISA

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

   Insert

   nanobody Nb_TM#2
   

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3_SARS-CoV-Spike[SARS-CoV-2 RBD]

   Plasmid

   #190086

   Purpose

   express chimeric SARS-CoV spike with the SARS-CoV-2 receptor binding domain

   Depositor

   Kenneth Matreyek

   Article

   Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

   Insert

   SARS-CoV S (S Synthetic)
   

   Tags

   Rhodopsin C9

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTY153 EGFP-T2A-ORF6 SARS-CoV-2

   Plasmid

   #204975

   Purpose

   Co-express EGFP and SARS-CoV-2 ORF6

   Depositor

   Tim Mitchison

   Article

   Yoo et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

   Insert

   Open Reading Frame 6 (ORF6 SARS-CoV-2)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEnt R3L2 TetO(sh) mCh-Rs1-2

   Plasmid

   #24417

   Depositor

   Edward Hsiao

   Article

   Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

   Insert

   Rs1
   

   Use

   Entry vector

   Tags

   FLAG and mCherry

   Mutation

   Human 5HT4B receptor with D100A mutation, generat…

   Available Since

   March 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET51b(+)-NADPH-LUCID-2 (C50 29 nM)

   Plasmid

   #117909

   Purpose

   Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 29 nM

   Depositor

   Kai Johnsson

   Article

   Yu et al Science. 2018 Sep 14;361(6407):1122-1126

   Insert

   SNAP_Pro30_[cpNLuc23_24]eDHFR(R98A)
   

   Tags

   His-tag and Strep-tag

   Expression

   Bacterial

   Mutation

   R98A on eDHFR

   Promoter

   T7

   Available Since

   Oct. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. p2Flag CMV2-ZO-2-1st-PDZ-mut

   Plasmid

   #27416

   Depositor

   Marius Sudol

   Article

   Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

   Insert

   Zonula Occludens 2 (TJP2 Human)
   

   Tags

   2xFlag

   Expression

   Mammalian

   Mutation

   K38E, F44H, I46E and V48E

   Available Since

   April 12, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pXD70EN19-Pct5-GPA_25810/25800-(+)-Eldh1/2(GpDSM19378)

   Plasmid

   #225305

   Purpose

   Expresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 and (+)-Eldh2 in Eggerthella lenta DSM 2243 with a cumate inducible promoter

   Depositor

   Emily Balskus

   Article

   Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

   Insert

   (+)-Eldh1, (+)-Eldh2
   

   Expression

   Bacterial

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. CA-0211 CMVTO-hIL2-rens-del.2

    Plasmid

    #231228

    Purpose

    CleaveIL-2_loop3_del

    Depositor

    Xiaojing Gao

    Article

    Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

    Insert

    hIL2-rens-del.2 (IL2 Human)
    

    Expression

    Mammalian

    Mutation

    L80F, R81D, L85V, I86V, I92F

    Available Since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. TL1 alphaDdCBE 1-2 (right, DddA11-N)

    Plasmid

    #222000

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alpha-1/2 right)-TALE CTD-DddA11 1397N-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    TALE: Q231R, W232G, S233A | TALE: S1330I, A1341V,…

    Promoter

    CMV

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pVH321-2-Tier1-PhCMV-NLS-dCas9-3xNLS

    Plasmid

    #169596

    Purpose

    Tier-1 vector encoding PhCMV-driven NLS-dCas9-3xNLS expression (PhCMV-NLS-dCas9-3xNLS-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    dead S.pyogenes Cas9
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pU6-sgRNA EF1Alpha-puro-T2A-BFP.PARN-2

    Plasmid

    #128760

    Purpose

    Expresses PARN gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    PARN sgRNA #2 (PARN Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-2

    Plasmid

    #128757

    Purpose

    Expresses PAPD4 gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    PAPD4 sgRNA #1 (TENT2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZCS36 (Phsp16.41::Cas9(dpiRNA)::tbb-2 ‘3UTR)

    Plasmid

    #193048

    Purpose

    Heatshock expressed Cas9

    Depositor

    Patrick Phillips

    Article

    Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

    Insert

    hsp16.41p::Cas9(dpiRNA)::tbb-2 ‘3UTR
    

    Use

    CRISPR

    Expression

    Worm

    Promoter

    hsp16.41p

    Available Since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. 3341 pcDNA3 Bcl-2 T69A S70A S87A

    Plasmid

    #13340

    Depositor

    Stanley Korsmeyer

    Article

    Yamamoto et al Mol Cell Biol 1999 Dec;19(12):8469-78.

    Insert

    Bcl-2 (BCL2 Human)
    

    Expression

    Mammalian

    Mutation

    changed Threonine 69 to Alanine; changed Serine 7…

    Available Since

    Nov. 9, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pLV-SARS-CoV-2 SL1 alone-Luciferase

    Plasmid

    #191482

    Purpose

    Luciferase reporter for SARS-CoV-2 5' UTR SL1 alone

    Depositor

    Hao Wu

    Article

    Shi et al bioRxiv. 2020 Sep 18. doi: 10.1101/2020.

    Insert

    SARS-CoV-2 5' UTR SL1 alone
    

    Use

    Lentiviral and Luciferase

    Mutation

    Contains only the stem-loop1 region of SARS-CoV-2…

    Available Since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. ATF4 17: 5’ATF4.uORF1stopm1&2.GFP

    Plasmid

    #21863

    Depositor

    David Ron

    Article

    Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

    Insert

    ATF4 (Atf4 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Stop codons between the colinear uORF1 and uORF2 …

    Available Since

    Sept. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-2)

    Plasmid

    #159657

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Promoter

    Ef1a

    Available Since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZLCv2-gD4Z4-2-3xFLAG-dCas9-HA-2xNLS

    Plasmid

    #106353

    Purpose

    CRISPR/Cas9 engineered chromatin immunoprecipitation of D4Z4 locus

    Depositor

    Stephen Tapscott

    Article

    Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

    Insert

    D4Z4 gRNA-2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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