165,775 results

1. pHRm-NLS-dCas9-GFP11x7-NLS

   Plasmid

   #70224

   Purpose

   Expresses NLS-dCas9-GFP11x7-NLS in mammalian cells

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Insert

   NLS-dCas9-GFP11x7-NLS
   

   Tags

   GFP11x7

   Expression

   Mammalian

   Available Since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. VP12

   Plasmid

   #72247

   Purpose

   Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A and Q926A

   Promoter

   CMV

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. Flag-neo-PP1-Alpha

   Plasmid

   #171267

   Purpose

   Mammalian expression of 3x-Flag-tagged human PP1 alpha catalytic subunit

   Depositor

   Steven Balk

   Article

   Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

   Insert

   3x-Flag-tagged human PP1 alpha catalytic subunit (PPP1CA Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTriEx-HTNC

   Plasmid

   #13763

   Purpose

   For expression and purification of a His-TAT-NLS-Cre fusion; HIV-TAT promotes cellular uptake of recombinant Cre into mammalian cells.

   Depositor

   Klaus Rajewsky

   Article

   Peitz et al Proc Natl Acad Sci U S A. 2002 Apr 2. 99

   Insert

   His-TAT-NLS-Cre
   

   Use

   Baculovirus

   Tags

   His Tag, NLS, and TAT

   Expression

   Bacterial

   Promoter

   p10 promoter

   Available Since

   April 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. AB-GFP

   Plasmid

   #72203

   Purpose

   screen AB42 variants for aggregation

   Depositor

   Michael Hecht

   Article

   Wurth et al J Mol Biol. 2002 Jun 21;319(5):1279-90.

   Insert

   AB42 (APP Human)
   

   Tags

   GFP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti HRE-Luc pGK Hygro

   Plasmid

   #118706

   Purpose

   reporter plasmid HIF-responsive promoter (3xHRE) wt- fused to firefly luciferase

   Depositor

   William Kaelin

   Article

   Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

   Insert

   HRE (HIF-responsive promoter (3xHRE)-luc
   

   Use

   Lentiviral

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-PGK-EGFP-ALDH1A2-Neo

   Plasmid

   #189743

   Purpose

   The construct was used to express GFP tagged human ALDH1A2 in mammalian cells for the analysis of the subcellular location of this protein.

   Depositor

   Robert Sobol

   Article

   Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

   Insert

   ALDH1A2 (ALDH1A2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-PE-Plus

   Plasmid

   #214079

   Purpose

   Mammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and IRES-human MLH1dn

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Insert

   PE-Plus
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-RIαΔ(D/D+linker)-EGFP

   Plasmid

   #181843

   Purpose

   GFP-tagged PKA RIα subunit lacking the docking/dimerization (D/D) and linker regions; for mammalian expression.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   RIα-(D/D+linker)EGFP (PRKAR1A Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Deletion of residues 12-112 in PKA RIα.

   Promoter

   CMV

   Available Since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. PEA1-Puro

    Plasmid

    #171991

    Purpose

    Delivers all prime editing nickase components in a single, puromycin selectable plasmid

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    

    Expression

    Mammalian

    Promoter

    CMV for Cas9, U6 for gRNAs

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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