We narrowed to 2,171 results for: Xpa

1. pTECH-MmAcK3RS(IPYE)

   Plasmid

   #104071

   Purpose

   Expression plasmid encoding MmAcK3RS(IPYE) and pylT

   Depositor

   David Liu

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   Plpp MmAcK3RS(IPYE); PproK pyl
   

   Expression

   Bacterial

   Mutation

   Contains activating mutations V31I, T56P, H62Y, A…

   Promoter

   Plpp and PproK

   Available Since

   Dec. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMC-1-BeYDV-LS-L-11

   Plasmid

   #197717

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   BeYDV LSL in c-sense with GG expansion to v-sense ORF position
   

   Expression

   Plant

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMC-1-BeYDV-LRS-L-11

   Plasmid

   #197718

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   BeYDV LRSL in c-sense with GG expansion to v-sense ORF position
   

   Expression

   Plant

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRF0G-IodoY

   Plasmid

   #113645

   Purpose

   Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging iodotyrosine at amber stop codons.

   Depositor

   Jeffrey Barrick

   Article

   Monk et al ACS Synth Biol. 2016 Sep 20.

   Insert

   IodoYRS and tRNA gene
   

   Use

   Synthetic Biology

   Available Since

   July 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pMC-1-WDV-LRS-L-11

   Plasmid

   #197720

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   WDV LRSL in c-sense with GG expansion to v-sense ORF position
   

   Expression

   Plant

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Mut2-RS

   Plasmid

   #182882

   Purpose

   A pEvol-based plasmid containing one inducible (by arabinose) copy of the Mut2-RS aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.

   Depositor

   Miriam Amiram

   Article

   Hadar et al Chembiochem. 2021 Apr 16;22(8):1379-1384

   Insert

   Mut2-RS
   

   Expression

   Bacterial

   Available Since

   July 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. UAS-Cre-T2A-miRFP670

   Plasmid

   #193581

   Purpose

   Fluorescent reporter for Gal4-induced Cre recombination

   Depositor

   Satoru Takahashi

   Article

   Yoshihara et al Exp Anim. 2022 Aug 5;71(3):385-390. doi:

   Insert

   miRFP670
   

   Use

   Cre/Lox

   Tags

   T2A

   Promoter

   5X UAS

   Available Since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMC-1-WDV-LS-L-11

   Plasmid

   #197719

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   WDV LSL in c-sense with GG expansion to v-sense ORF position
   

   Expression

   Plant

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAWP78-PVCpnf_pvc13-Ad5RGDPK7_Δpvc10

   Plasmid

   #198449

   Purpose

   Produces PVCs retargeted with expanded tropism hAd5 knob (RGD and PK7 insertions); also contains truncated spike tip protein (Pvc10).

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   PVCpnf_pvc13-Ad5RGDPK7_Δpvc10
   

   Expression

   Bacterial

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKPY197

    Plasmid

    #62599

    Purpose

    Encodes Thr412Gly-CePheRS Alpha Subunit

    Depositor

    David Tirrell

    Article

    Yuet et al Proc Natl Acad Sci U S A. 2015 Feb 17. p

    Insert

    Thr412Gly-CePheRS Alpha Subunit
    

    Tags

    HIS

    Expression

    Worm

    Mutation

    Thr412Gly

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAcBac1-3nitroY-A7-RS

    Plasmid

    #141173

    Purpose

    Expression of orthogonal tRNA and tRNA synthetase for incorporation of unnatural amino acids

    Depositor

    Ryan Mehl

    Article

    Porter et al ACS Chem Biol. 2019 Jun 21;14(6):1328-13

    Insert

    A7 3nY tRNA synthetase
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    P186T L274R N311G C313N

    Promoter

    CMV

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRSF-ChPylTMSK

    Plasmid

    #163915

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of N6-(((Trimethylsilyl)methoxy)carbonyl)-L-lysine (TMSK), into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Abdelkader et al J Am Chem Soc. 2021 Jan 5. doi: 10.1021/

    Insert

    tRNA synthetase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0

    Plasmid

    #174081

    Purpose

    Expression of orthogonal Mb tRNA and tRNA synthetase for incorporation of tetrazine3.0 in eukaryotic cells.

    Depositor

    Ryan Mehl

    Article

    Jang et al J Am Chem Soc. 2020 Apr 22;142(16):7245-

    Insert

    NES-Flag-R2-84 M. barkeri tRNA synthetase for tetrazine3.0
    

    Tags

    NES-Flag

    Expression

    Mammalian

    Mutation

    L270G, Y271L, N311G, C313A

    Promoter

    CMV

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIDTSmart-MbPylRS

    Plasmid

    #217361

    Purpose

    Expresses Methanosarcina barkeri aminoacyl-tRNA synthetases; can be packaged into bacmid

    Depositor

    Abhishek Chatterjee

    Article

    Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

    Insert

    M. barkeri pyrrolysyl-tRNA synthetase
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEVOL-pylT-N346A/C348A

    Plasmid

    #127411

    Purpose

    Double mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethers

    Depositor

    Wenshe Liu

    Article

    Wang et al J Am Chem Soc. 2012 Feb 15;134(6):2950-3

    Insert

    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    

    Expression

    Bacterial

    Mutation

    N346A-C348A

    Promoter

    araBAD

    Available Since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIRE4-Azi

    Plasmid

    #105829

    Purpose

    Plasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cells

    Depositor

    Irene Coin

    Article

    Seidel et al Elife. 2017 Aug 3;6. doi: 10.7554/eLife.

    Inserts

    Humanized EAziRS
    4 copies of the U6-BstYam tRNA cassette
    

    Expression

    Mammalian

    Mutation

    (Y37L, D182S, F183M, L186A, D265R)TyrRS

    Promoter

    PGK and U6

    Available Since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pANAP

    Plasmid

    #48696

    Purpose

    Allows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRS

    Depositor

    Peter Schultz

    Article

    Chatterjee et al J Am Chem Soc. 2013 Aug 28;135(34):12540

    Inserts

    8 copies of the amber suppressor tRNACUA-Leu
    AnapRS
    

    Expression

    Mammalian

    Mutation

    mutant E.coli leucyl synthetase specific for Anap…

    Promoter

    CMV and human H1 promoter

    Available Since

    Nov. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSUP-(2xCouRS-tRNAopt)

    Plasmid

    #197930

    Purpose

    Machinery plasmid expressing constitutive and inducible 7HCou synthetase and cognate amber suppressing tRNA. Incorporates (7-hydroxy-4-coumarin-yl) ethylglycine

    Depositor

    Kenneth Johnson

    Article

    Dangerfield et al J Biol Chem. 2020 Dec 11;295(50):17265-1

    Inserts

    CouRS
    tRNA opt
    

    Expression

    Bacterial

    Promoter

    glnS' and lambda(ts) and proK

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMAH-POLY-eRF1(E55D)

    Plasmid

    #157925

    Purpose

    Encodes a polyspecific aminoacyl-tRNA synthetase fused to a modified releasing factor and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells

    Depositor

    Huiwang Ai

    Article

    Chen et al Cell Chem Biol. 2021 Jan 27. pii: S2451-

    Insert

    POLY-eRF1(E55D), Polyspecific aminoacyl-tRNA Synthetase fused to a modified releasing factor through 2A peptide
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDule-tfmF A65V S158A

    Plasmid

    #85484

    Purpose

    Machinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNA

    Depositor

    Ryan Mehl

    Article

    Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

    Insert

    tri-fluoromethyl-phenylalanine Mj synthetase A65V S158A
    

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32V F108W Q109M I159M with 65Val and 158Ala

    Promoter

    lpp (constitutive)

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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