We narrowed to 5,749 results for: actin

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ERoxBFP

Plasmid

#68126

Purpose

mammalian expression of ER localized oxBFP

Depositor

Erik Snapp

Article

Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

Insert

oxBFP

Tags

KDEL ER retention sequence and bovine prolactin s…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only
E407V Nuclear Myosin I YFP

Plasmid

#60242

Purpose

Expresses a E407V mutant of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding and motor activities

Depositor

Primal de Lanerolle

Article

Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.

Insert

Nuclear Myosin 1 (Myo1c Mouse)

Tags

EYFP and NLS

Expression

Mammalian

Mutation

E407V

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only
pAAV.CAG.GCaMP6m.WPRE.SV40

Plasmid

#100840

Purpose

AAV expression of ultrasensitive protein calcium sensor from CAG promoter

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Has Service

AAV1

Insert

GCaMP6m

Use

AAV

Expression

Mammalian

Mutation

GCaMP3-T302L R303P M378G K379S D380Y T381R S383T …

Promoter

CAG

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pLPC-MYC-hTRF1

Plasmid

#64164

Purpose

Retroviral vector expressing human TRF1 with N-terminal MYC tag

Depositor

Titia de Lange

Article

Zimmermann et al Genes Dev. 2014 Nov 15;28(22):2477-91. d

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223-RIPK2

Plasmid

#23846

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RIPK2 (RIPK2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-HIPK2

Plasmid

#23481

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

HIPK2 (HIPK2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pCMV4-HA

Plasmid

#27553

Depositor

Shao-Cong Sun

Article

Xiao et al J Biol Chem. 2000 Jul 14. 275(28):21081-

Type

Empty backbone

Tags

3x HA

Expression

Mammalian

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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GFP-α-cateninV-

Plasmid

#229696

Purpose

Transient expression of GFP-alpha-cateninV- in mammalian cells

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

CTNNA1 (alpha-catenin) Human (CTNNA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

L344P

Promoter

CMV promoter

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgABI2-1

Plasmid

#210139

Purpose

knock out ABI2 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Abl interactor 2 (ABI2 Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgCYFIP1-1

Plasmid

#210137

Purpose

knock out CYFIP1 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Cytoplasmic FMR1-interacting protein 1 (CYFIP1 Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgABI2-2

Plasmid

#210140

Purpose

knock out ABI2 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Abl interactor 2 (ABI2 Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgHSPC300-1

Plasmid

#210141

Purpose

knock out HSPC300 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Protein BRICK1 (BRK1 Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgHSPC300-2

Plasmid

#210142

Purpose

knock out HSPC300 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Protein BRICK1 (BRK1 Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-SPN_ARR N52R (Shank3)

Plasmid

#175255

Purpose

SPN-ARR fragment with structure opening N52R mutation in EGFP backbone for mammalian expression

Depositor

Johanna Ivaska

Article

Salomaa et al Curr Biol. 2021 Sep 29. pii: S0960-9822(

Insert

SPN-ARR fragment with N52R mutation from SHANK3

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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human TLNRD1-4H pET151

Plasmid

#159386

Purpose

Expresses human TLNRD1 4-helix domain (residues 143-273) in bacterial cells

Depositor

Ben Goult

Article

Cowell et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

Talin Rod Domain Containing Protein 1 (TLNRD1 Human)

Tags

his-tag, TEV cleavage site

Expression

Bacterial

Mutation

TLNRD1 4-helix domain (residues 143-273)

Promoter

T7

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pHD-dsRed-attP_dCrk_flankingHRs

Plasmid

#131141

Purpose

Contains ~1kb homology regions flanking Drosophila crk locus. Used to make crk null mutant. Homology regions cloned into AarI and SapI sites of pHD-dsRed-attP (Harrison, O'Connor-Giles, Wildonger).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Inserts

Crk Upstream Homology Region (Crk Fly)
Crk Downstream Homology Region (Crk Fly)

Use

CRISPR and Cre/Lox; Homology directed repair temp…

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-chiRNA_dCrkgRNA1

Plasmid

#131139

Purpose

Encodes gRNA targeting Drosophila crk locus. Cuts 110bp upstream of the transcription start site. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

dCrk gRNA1 (Crk Fly)

Use

CRISPR

Expression

Insect

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-chiRNA_dCrkgRNA2

Plasmid

#131140

Purpose

Encodes gRNA targeting Drosophila crk locus. Cuts 99bp beyond the 3' UTR. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

dCrk gRNA1 (Crk Fly)

Use

CRISPR

Expression

Insect

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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hROSA26 CRISPR-pX330

Plasmid

#105927

Purpose

hROSA26 targeting CRISPR plasmid

Depositor

Masato Kanemaki

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

hROSA26 gRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pclbw-opa1(isoform 1)-myc

Plasmid

#62845

Purpose

Mammalian Expression of myc-tagged Opa1 isoform1

Depositor

David Chan

Article

Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

Insert

mmOPA1isoform1-myc (Opa1 Mouse)

Use

Retroviral

Tags

9xMyc

Expression

Mammalian

Promoter

cmv-bactin

Available Since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only