We narrowed to 5,304 results for: pcDNA3.1(+)

1. pcDNA3.1-FLAG-SBP-STAG2 (V181M)

   Plasmid

   #73971

   Purpose

   STAG2 cDNA (CCDS43990) V181M

   Depositor

   Todd Waldman

   Article

   Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

   Insert

   STAG2 (STAG2 Human)
   

   Use

   Tags

   FLAG-SBP

   Expression

   Mammalian

   Mutation

   V181M

   Promoter

   Available Since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

2. NC11 pCDNA3.1 VENUS WTX K292N

   Plasmid

   #36966

   Depositor

   Randall Moon

   Article

   Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

   Insert

   WTX K292N (AMER1 Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   K292N

   Promoter

   CMV

   Available Since

   June 20, 2013

   Availability

   Academic Institutions and Nonprofits only

3. NC8 pCDNA3.1 VENUS WTX S286D

   Plasmid

   #36963

   Depositor

   Randall Moon

   Article

   Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

   Insert

   WTX S286D (AMER1 Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   S286D

   Promoter

   CMV

   Available Since

   Sept. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

4. Bcr del oligo pCDNA3.1/HisB

   Plasmid

   #36423

   Depositor

   Nora Heisterkamp, John Groffen

   Article

   Yi et al BMC Biochem. 2011 Feb 10;12:8.

   Insert

   BCR (BCR Human)
   

   Use

   Tags

   Xpress

   Expression

   Mammalian

   Mutation

   Deletion of amino acid residues 2-63

   Promoter

   Available Since

   Aug. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

5. NC7 pCDNA3.1 VENUS WTX S286A

   Plasmid

   #36962

   Depositor

   Randall Moon

   Article

   Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

   Insert

   WTX S286A (AMER1 Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   S286A

   Promoter

   CMV

   Available Since

   Aug. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

6. NC10 pCDNA3.1 VENUS WTX T289A

   Plasmid

   #36965

   Depositor

   Randall Moon

   Article

   Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

   Insert

   WTX T289A (AMER1 Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   T289A

   Promoter

   CMV

   Available Since

   July 24, 2012

   Availability

   Academic Institutions and Nonprofits only

7. NC9 pCDNA3.1 VENUS WTX S286E

   Plasmid

   #36964

   Depositor

   Randall Moon

   Article

   Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

   Insert

   WTX S286E (AMER1 Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   S286E

   Promoter

   CMV

   Available Since

   July 24, 2012

   Availability

   Academic Institutions and Nonprofits only

8. Kv7.4 (1-645, Δ368-492)/pcDNA3.1

   Plasmid

   #111454

   Purpose

   Electrophysiology in HEK cell

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Kv7.4 (1-645, Δ368-492), C643A

   Promoter

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-Cerulean-Epac2(R53A)-Venus

   Plasmid

   #138197

   Purpose

   FRET-based Epac2 activation sensor with R53A mutation in Epac2 coding sequence.

   Depositor

   Jin Zhang

   Article

   Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

   Insert

   Cerulean-Epac2(R53A)-Venus (Rapgef4 Synthetic, Mouse)
   

   Use

   Tags

   Cerulean and Venus

   Expression

   Mammalian

   Mutation

   Contains arginine-to-alanine substitution at resi…

   Promoter

   CMV

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-Cerulean-Epac2(R466A)-Venus

    Plasmid

    #138199

    Purpose

    FRET-based Epac2 activation sensor with R466A mutation in Epac2 coding sequence.

    Depositor

    Jin Zhang

    Article

    Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

    Insert

    Cerulean-Epac2(R466A)-Venus (Rapgef4 Synthetic, Mouse)
    

    Use

    Tags

    Cerulean and Venus

    Expression

    Mammalian

    Mutation

    Contains arginine-to-alanine substitution at resi…

    Promoter

    CMV

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-Cerulean-Epac2(L426W)-Venus

    Plasmid

    #138200

    Purpose

    FRET-based Epac2 activation sensor with L426W mutation in Epac2 coding sequence.

    Depositor

    Jin Zhang

    Article

    Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

    Insert

    Cerulean-Epac2(L426W)-Venus (Rapgef4 Synthetic, Mouse)
    

    Use

    Tags

    Cerulean and Venus

    Expression

    Mammalian

    Mutation

    Contains leucine-to-tryptophan substitution at re…

    Promoter

    CMV

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-dCas9-Dnmt3a-Dnmt3l-P2A-eGFP

    Plasmid

    #128424

    Purpose

    Human Codon Optimized dCas9-DNMT3A3L

    Depositor

    Bradley Bernstein

    Article

    Tarjan et al Nat Commun. 2019 Sep 18;10(1):4258. doi:

    Insert

    dCas9m4-sv40NLS-DNMT3A-3L-sv40NLS-P2A-eGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    T7 Promoter

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3.1(+) miniTurboID-6xGGSG linker-FKBPF36V-2xHA

    Plasmid

    #200641

    Purpose

    Expresses miniTurboID-FKBPF36V-2xHA in mammalian cells.

    Depositor

    Fleur Ferguson, Justin English

    Article

    Tao et al Nat Commun. 2023 Dec 4;14(1):8016. doi:

    Insert

    miniTurboID-6xGGSG linker-FKBPF36V-2xHA
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-EF1A-mNeonGreen2x-SREBP1-P2A-Puro

    Plasmid

    #228401

    Purpose

    Encodes a N-terminal truncated version of SREBP1 lacking part of the transcriptional activation domain and fused to a tandem of mNeongreen to visualize the import of SREBP1

    Depositor

    Dirk Daelemans

    Article

    Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

    Insert

    SREBP1 (SREBF1 Human)
    

    Use

    Tags

    mNeonGreen2x

    Expression

    Mammalian

    Mutation

    deleted N terminal activation domain (amino acids…

    Promoter

    EF1a

    Available Since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

    Plasmid

    #182653

    Purpose

    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Inserts

    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    mutant eukaryotic release factor 1
    

    Use

    Tags

    HA tag and nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    E55D and Y306A/Y384F (AF) double mutant of Methan…

    Promoter

    CMV and U6

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-HaloAKAR2.1-NES-T2A-EGFP-CAAX

    Plasmid

    #216491

    Purpose

    Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) and PM targeted EGFP in mammalian cells

    Depositor

    Jin Zhang

    Article

    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

    Insert

    HaloAKAR2.1
    

    Use

    Tags

    NES, T2A-EGFP-CAAX

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(+)_4x(U6 tRNA M15)_CMV NESPylRS(AF)

    Plasmid

    #182652

    Purpose

    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & 4x M15 tRNACUA used for amber codon suppression in mammalian cells

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Inserts

    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    4xU6-tRNAM15
    

    Use

    Tags

    nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    Y306A/Y384F (AF) double mutant of Methanosarcina …

    Promoter

    CMV and U6

    Available Since

    May 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(+)-myc-OGA(GS-544)C181-D174N

    Plasmid

    #193999

    Purpose

    plasmid encoding myc-tagged OGA(GS-544)C181-D174N

    Depositor

    Christina Woo

    Article

    Ge et al ACS Chem Biol. 2023 Jan 4. doi: 10.1021/

    Insert

    myc-OGA(GS-544)C181-D174N
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. (114) pcDNA3.1-GFP-Flag-JunB-EPEA

    Plasmid

    #160743

    Purpose

    Expresses a GFP tagged JunB with Flag and EPEA tags.

    Depositor

    Christina Woo

    Article

    Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

    Insert

    GFP-Flag-JunB-EPEA (JUN Human)
    

    Use

    Tags

    EPEA, Flag, and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available Since

    May 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-COXIV-COX8-dL5-2XG4S-mCer3

    Plasmid

    #73208

    Purpose

    Expresses dL5(E52D)-mCer3 fusion protein in mitochondria of mammalian cells. (MBIC5, dL5**, FAP)

    Depositor

    Marcel Bruchez

    Article

    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46

    Insert

    COXIV-COX8-dL5-2XG4S-mCer3 (COX8A Synthetic, Human)
    

    Use

    Tags

    The FAP and mCerulean3 are fused with 2 copies of…

    Expression

    Mammalian

    Mutation

    The dL5** FAP is E50D, L89S, also known as E52D/L…

    Promoter

    CMV

    Available Since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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