We narrowed to 1,249 results for: vent

1. pEMS1132

   Plasmid

   #29053

   Purpose

   High copy homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving an EGFP/Cre fusion reporter

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

   Insert

   Ple67 (FEV Human)
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   EGFP-Cre-NLS

   Expression

   Mutation

   Promoter

   Available since

   Jan. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated

   Plasmid

   #215769

   Purpose

   encodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locus

   Depositor

   Dimitrios Wagner

   Article

   Glaser et al Genome Biol. 2023 Apr 24;24(1):89. doi:

   Inserts

   TRAC-5' homology arm (TRAC Human)
   P2A-CD19.28.zeta-bgH (CD19 Human, Mouse, Bovine)
   TRAC-3' homology arm (TRAC Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Cas12a PAM mutated

   Promoter

   Available since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3-Flag-SPRTN-wt

   Plasmid

   #110214

   Purpose

   Expression in mammalian cells of full-length wild-type SPRTN protein with a Flag-tag at N-terminus.

   Depositor

   Kristijan Ramadan, Ivana Marinovic-Terzic

   Article

   Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:

   Insert

   SPRTN (SPRTN Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   P296L (see depositor comments below)

   Promoter

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCI AscI MR1 R9H res

   Plasmid

   #214753

   Purpose

   expresses human MR1 R9H resistant to CRISPR editing with a specific sgRNA in mammalian cells

   Depositor

   David Lewinsohn

   Article

   Kulicke et al Commun Biol. 2024 Feb 24;7(1):228. doi:

   Insert

   MHC class I-related protein 1 (MR1 Human)
   

   Use

   Tags

   IRES eGFP

   Expression

   Mammalian

   Mutation

   silent mutations to prevent editing by CRISPR/Cas…

   Promoter

   CMV

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTL

   Plasmid

   #203176

   Purpose

   Centromeric yeast expression vector, leucine selection

   Depositor

   William Sessa

   Article

   Galosi et al Brain. 2022 Mar 29;145(1):208-223. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHD-dsRed-attP_dCrk_flankingHRs

   Plasmid

   #131141

   Purpose

   Contains ~1kb homology regions flanking Drosophila crk locus. Used to make crk null mutant. Homology regions cloned into AarI and SapI sites of pHD-dsRed-attP (Harrison, O'Connor-Giles, Wildonger).

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Inserts

   Crk Upstream Homology Region (Crk Fly)
   Crk Downstream Homology Region (Crk Fly)
   

   Use

   CRISPR and Cre/Lox; Homology directed repair temp…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3-Flag-SPRTN-E112A

   Plasmid

   #110215

   Purpose

   Expression in mammalian cells of of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, and Flag-tag at N-terminus.

   Depositor

   Kristijan Ramadan, Ivana Marinovic-Terzic

   Article

   Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:

   Insert

   SPRTN (SPRTN Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   E112A, P296L (see depositor comments below)

   Promoter

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTL-DHDDS

   Plasmid

   #203177

   Purpose

   Centromeric yeast expression vector for hDHDDS

   Depositor

   William Sessa

   Article

   Galosi et al Brain. 2022 Mar 29;145(1):208-223. doi:

   Insert

   DHDDS (DHDDS Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   RER2

   Available since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUb FLAG-tws

   Plasmid

   #195068

   Purpose

   Expresses FLAG-tagged Drosophila tws protein

   Depositor

   Jeremy Wilusz

   Article

   Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

   Insert

   twins (tws Fly)
   

   Use

   Tags

   3xFLAG

   Expression

   Insect

   Mutation

   Promoter

   Ubi-p63e

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGB-GST-3C-mCherry-ATG14/Vps34/Vps15/BECN1

    Plasmid

    #187936

    Purpose

    Plasmid for the expression and purification of GST-3C-mCherry-ATG14/Vps34/Vps15/BECN1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1327

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Inserts

    ATG14 (ATG14 Human)
    VPS34 (PIK3C3 Human)
    VPS15 (PIK3R4 Human)
    BECN1 (BECN1 Human)
    

    Use

    Tags

    GST-mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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