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We narrowed to 481 results for: vent

Showing: 1 - 20 of 481 results

  1. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...compared vented and non-vented tubes to controls that were not exposed to dry ice. Does venting help? We... shown). What we did next: Venting the tubes We then tested whether venting the tubes prior to thaw released...stored samples on dry ice for 72 hours and then vented the tubes by completely removing the caps, incubating... We found that 30 seconds of venting attenuated the pH change caused by storage on dry ice (Fig. 3). However...pH (from 7.4 to 6.8). It’s possible that a longer vent time would be sufficient to return the sample back...However, if this is not possible, we recommend both venting the tube for at least 30 seconds prior to thaw,... effect is reversible and the pH shift can be prevented. Keep this information in mind if you’re planning...

  2. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...requires special considerations. Conventional flow cytometry During conventional flow cytometry, light emitted...up to 64 markers in a single panel! Although conventional flow cytometry and spectral flow cytometry are... series of mirrors and filters. Importantly, conventional flow cytometry uses one detector to detect one...emission spectra and filters for FITC and PE on a conventional flow cytometer.   Spectral flow cytometry...fluorophores that cannot be distinguished using conventional flow can (sometimes) be distinguished using ...Using spectral flow When deciding whether to use conventional or spectral flow cytometry for a given experiment...negative populations, Careful panel design In both conventional and spectral flow cytometry, it is important...

  3. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...detection of mycoplasmas difficult (Ligasová, 2019). Prevention of mycoplasma contamination The best and the ...humans are the major source of contamination, the prevention steps start from you. If you are careful enough...contamination but it’s a very good practice for prevention. Prepare a small quantity of media stock that...that would be used for the experiment, preventing contamination of the rest. Avoid long exposure of the ...only be used to remove mycoplasma and not for preventing mycoplasma growth. Also, prolonged use of a particular...Conclusion To sum up, it is always important to prevent contamination rather than eradicating it later....can and help them understand the importance of preventing contamination. Be aware and cautious rather than...

  6. CRISPR Library Amplification

    Type
    Protocol
    ...using a new vented falcon tube for every two transformations. Each of the four 14 mL Vented Falcon Tubes...use 350 mL of LB Agar per bioassay plate. 14 mL Vented Falcon Tubes (BD Biosciences, 352059) 3X LB Agar...freezer. Aliquot 3 mL SOC into each of four 14 mL Vented Falcon Tubes and have 1 mL SOC per electroporation...Remove all liquid from cuvette and add to 14 mL vented Falcon Tube containing 3 mL SOC. Repeat for each...transformed DNA-Endura from cuvette. Shake four 14 mL Vented Falcon Tubes at 30-37 ℃, 225 rpm for 1 hour. After...

  8. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ... and make sure that clean air is flowing in. The vent for the in-flowing air is normally visible and you...before entering the hood is another great way to prevent contamination. This sounds rather trivial, but ...closed when they aren’t in immediate use. This will prevent spills, but, if any spills do occur, they should...

  9. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...HEK293 cells Recombinant antibody plasmid DNA 500 mL vented flask 50 mL conical tubes Microcentrifuge tubes...at a density of 0.9 x 10 6 cells/mL in a 500 mL vented flask.Incubate in a 37 °C, 5% CO 2 incubator on...

  10. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...blunt-ended PCR products (e.g. Phusion, Pfu, and Vent polymerases). Note that certain polymerases such...tailored manner. The approach can be used in conventional cloning (to introduce or remove restriction ...

  11. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...separate DNA molecules (in trans), a translocation event is generated between the target sites. Created with...cassette) placed upstream of a gene of interest will prevent gene expression in the absence of Cre. In the presence...combined in multiple, independent recombination events in a single experimental system, enabling multi-layered... the target site cassette in a double-crossover event. Toxicity Cre-lox recombination is a very useful...lineage-specific drivers can also help reduce off-target events and the systemic effects of Cre toxicity. Leakiness...iCre variant, reduces the number of recombination events but can improve specificity in targeted neuronal...

  12. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...hU6 none S. pyogenes Ventura pDonor_mU6 69350 Mammalian mU6 none S. pyogenes Ventura pDonor_sU6 69351 Mammalian...64073 Mammalian BbsI and BsaI yes, cut S. pyogenes Ventura PX851 62883 Mammalian BbsI yes, cut, N-terminal...Mammalian sU6 none S. pyogenes Ventura pCRISPRyl 70007 Other/Yarrowia lipolytica SCR1'-tRNA yes, cut S...independent U6 promoters and Cas9 from CBh promoter. Ventura A CRISPR/Cas9 toolkit for multiplex genome editing...

  13. Brain Initiative Collection

    Type
    Collection
    ...will provide novel ways to treat, cure, and even prevent brain disorders. The BRAIN Initiative supports ... coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f. Edward Boyden 158757-AAV9 pAAV-CAG-... coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f. Edward Boyden 163505-PHPeB CN1390-rAAV-DLX2.0...

  14. Serotype Testing AAV

    Type
    Collection
    ...experiments. For more information, see our full AAV inventory or our AAV production page . Note: For a list ...100 µL sized sample, please see our Control AAV Inventory . The catalog ID for the 20 µL sized testing sample...

  15. CRISPR Guide

    Type
    Collection
    ...RuvC and HNH, can be altered to reduce cleavage events to a single cut (nick) or no cuts. Cas9 nickase...the donor template, incorporating mutations that prevent further Cas9 cleavage will improve the accuracy...and NGS (other genomic regions). Compared to conventional methods for genomic purification, CRISPR-based...Rekhtman, N., De Stanchina, E., Lowe, S. W., & Ventura, A. (2014). In vivo engineering of oncogenic chromosomal...

  16. Neurodegeneration Research Collection

    Type
    Collection
    ...understood, and there are no effective therapies to prevent, treat, or cure most of these disorders. An integrated...age of 60. There is presently no cure or way to prevent progression of PD. Treatment consists mainly of...

  17. CRISPR Plasmids - Tagging

    Type
    Collection
    ...principle of the PITCh system is different from the conventional HDR-mediated gene knock-in. The detailed procedure...and a repair template for an individual tagging event. The kit contains 26 plasmids; 21 of the plasmids...

  18. Bacterial Expression Systems

    Type
    Collection
    ...Plasmids Reporter plasmids can be used to detect events and molecules in and around a bacterium. Plasmids...genetic inversion, deletions, and translocations events. Visit our TALEN Collection page to find plasmids...

  19. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...using various delivery methods including intracerebroventricular (ICV) delivery in newborns, and retro-orbital...minBG-SYFP2-WPRE3-BGHpA AiP14470 AiE0027m_3xC3 SYFP2 Ventromedial hypothalamic nucleus (VMH) Hypothalamus 230531...

  20. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...macropinocytosis by lowering submembranous pH and preventing Rac1 and Cdc42 signaling. J Cell Biol. 2010 Feb...Single action potentials and subthreshold electrical events imaged in neurons with a fluorescent protein voltage...
Showing: 1 - 20 of 481 results