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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8 results for: vent

Showing: 1 - 8 of 8 results

  1. Lentiviral Vector Guide

    Type
    Guide
    ...lentiviruses has a long incubation period that eventually leads to serious disease and occurs in many species...regions, regions recently involved in translocation events, and other “fragile” genomic locations, and that...packaging plasmids. Unless multiple recombination events occur between the packaging, envelope, and transfer... incorporation into a host cell, this deletion prevents further transcription of the full-length virus...family. Characterized by long incubation and the eventual cause of serious diseases. Provirus The genetic...vectors, can occur from a spontaneous crossover event between the transfer plasmid and genes provided ...

  2. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...provided by the chosen cell line to ensure safety and prevent the potential formation of replication-competent...insertional mutagenesis. However, random integration events can occur at a very low frequency. As with most... Blood-brain barrier (BBB) Layer of cells that prevent the entry of toxins and pathogens from the blood...uses the cell machinery to produce new viruses, eventually lysing the cell to release them. Optogenetics...host cells as a result of a spontaneous crossover event between the rAAV vector and the adenoviral genes...

  3. Promoters

    Type
    Guide
    ... package DNA into nucleosomes. Histone binding prevents the initiation of transcription whereas transcription...are normally bound by a repressor protein that prevents transcription. When an inducer molecule binds ...to the repressor, it changes its conformation, preventing its binding to the operator and thus allowing...a repressor/corepressor binds to the operator preventing transcription. The trp operon involved in the...

  4. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...packaging plasmids. Unless multiple recombination events occur between the packaging, envelope, and transfer... incorporation into a host cell, this deletion prevents further transcription of the full-length virus...family. Characterized by long incubation and the eventual cause of serious diseases. Immunogenicity The ...vectors, can occur from a spontaneous crossover event between the transfer plasmid and genes provided ...

  5. Adenovirus Guide

    Type
    Guide
    ...purposes. The system consists of two plasmids that eventually recombine to form one: Shuttle/transfer plasmid...risk of oncogenesis. However, random integration events can occur at a very low frequency. As with most...Adenoviral vector immunity: its implications and circumvention strategies . Current Gene Therapy, 11 (4), 307...host cells as a result of a spontaneous crossover event between the adenoviral vector and the adenoviral...

  6. CRISPR Guide

    Type
    Guide
    ...RuvC and HNH, can be altered to reduce cleavage events to a single cut (nick) or no cuts. Cas9 nickase...the donor template, incorporating mutations that prevent further Cas9 cleavage will improve the accuracy...and NGS (other genomic regions). Compared to conventional methods for genomic purification, CRISPR-based...Rekhtman, N., De Stanchina, E., Lowe, S. W., & Ventura, A. (2014). In vivo engineering of oncogenic chromosomal...

  7. Chemogenetics Guide

    Type
    Guide
    ...23303063 (Link opens in a new window) Gomez JL, Bonaventura J, Lesniak W, Mathews WB, Sysa-Shah P, Rodriguez...Link opens in a new window) Magnus CJ, Lee PH, Bonaventura J, Zemla R, Gomez JL, Ramirez MH, Hu X, Galvan...

  8. Antibody Guide

    Type
    Guide
    ...block the membrane to remove excess antibody and prevent unwanted binding. Incubate the membrane with a ...
Showing: 1 - 8 of 8 results