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We narrowed to 413 results for: vent

Showing: 1 - 20 of 413 results

  1. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...compared vented and non-vented tubes to controls that were not exposed to dry ice. Does venting help? We... shown). What we did next: Venting the tubes We then tested whether venting the tubes prior to thaw released...stored samples on dry ice for 72 hours and then vented the tubes by completely removing the caps, incubating... We found that 30 seconds of venting attenuated the pH change caused by storage on dry ice (Fig. 3). However...pH (from 7.4 to 6.8). It’s possible that a longer vent time would be sufficient to return the sample back...However, if this is not possible, we recommend both venting the tube for at least 30 seconds prior to thaw,... effect is reversible and the pH shift can be prevented. Keep this information in mind if you’re planning...

  2. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    Published
    Aug. 3, 2023, 1:15 p.m.
    ...requires special considerations. Conventional flow cytometry During conventional flow cytometry, light emitted...up to 64 markers in a single panel! Although conventional flow cytometry and spectral flow cytometry are... series of mirrors and filters. Importantly, conventional flow cytometry uses one detector to detect one...emission spectra and filters for FITC and PE on a conventional flow cytometer.   Spectral flow cytometry...fluorophores that cannot be distinguished using conventional flow can (sometimes) be distinguished using ...Using spectral flow When deciding whether to use conventional or spectral flow cytometry for a given experiment...negative populations, Careful panel design In both conventional and spectral flow cytometry, it is important...

  3. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    Published
    Oct. 8, 2020, 1:15 p.m.
    ...detection of mycoplasmas difficult (Ligasová, 2019). Prevention of mycoplasma contamination The best and the ...humans are the major source of contamination, the prevention steps start from you. If you are careful enough...contamination but it’s a very good practice for prevention. Prepare a small quantity of media stock that...that would be used for the experiment, preventing contamination of the rest. Avoid long exposure of the ...only be used to remove mycoplasma and not for preventing mycoplasma growth. Also, prolonged use of a particular...Conclusion To sum up, it is always important to prevent contamination rather than eradicating it later....can and help them understand the importance of preventing contamination. Be aware and cautious rather than...

  6. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...furiosus, a hyperthermophile that exists in sea vents at the boiling point of water. Dr. Terns gave a ...studying how Argonaute pathways may be involved in preventing silencing of self-genes. Dr. Mello presented ...

  7. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ... and make sure that clean air is flowing in. The vent for the in-flowing air is normally visible and you...before entering the hood is another great way to prevent contamination. This sounds rather trivial, but ...closed when they aren’t in immediate use. This will prevent spills, but, if any spills do occur, they should...

  8. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...blunt-ended PCR products (e.g. Phusion, Pfu, and Vent polymerases). Note that certain polymerases such...tailored manner. The approach can be used in conventional cloning (to introduce or remove restriction ...

  9. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...Laura’s Top 10 Event Planning Tips! 1. Take suggestions Some of Addgene’s best events have been at the...way Fun events don't have to cost a lot, or anything at all really! We've had plenty of events that included...doing an event that requires people to be in groups, organize your troops prior to the event, and let ...feast and play lawn games. In previous years, this event was held at Executive Director Joanne Kamen’s home...the work vicinity over the weekend, even for an event where there is food and beer. Ha! We had over 60...rain towards the end, it was another successful event! Addgene company culture At Addgene we organically...effort to continue the tradition and plan enjoyable events for employees. We now have a team of three (myself...

  10. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ... approach to making the event interesting and engaging. Long before the event... Choose a moderator with...discussions are becoming a ubiquitous format for events and sessions in scientific conferences. They can... a different role so her ability to control the event is what is important. Choose 4 panelists (maybe ...and organizers should develop a list of 4-6 pre-event interview questions. The moderator (possibly with...and it will be impossible to schedule. In the pre-event interviews, the moderator should be taking good ...develop a final list of questions for the actual event, based on these interviews. The idea is to ask questions...and parking information and remind them about the event as it approaches. Room set-up and logistics To...

  11. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...off-target events There are several strategies to lower the predicted frequency of off-target events, many ... editing events will be on target. Thus, if you isolate/sequence/whatever your editing event outcome is...the way of ‘good enough’. Predicting off-target events As mentioned in the above section, there are gRNA...score candidate gRNAs for on-target and off-target event efficiencies. In addition to these tools generating... but also score their probability of off-target events: CRISPOR, Cas-OFFinder, CCTop, and many more. .... Of note, most of these optimized Cas enzymes prevent cutting at mismatched guide sites, but not necessarily... result in a DNA double strand break (DSB), the event required for frameshift and knock-in mutations. ...

  12. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ... - organic solvents and detergents.  Organic Solvents  As mentioned above, organic solvents can be used...with this type of fixation*. Organic Solvents Organic solvents, generally acetone, methanol, or ethanol...fixatives - chemical cross-linkers and organic solvents.  Chemical Cross-Linkers Chemical cross-linkers...cross-linking can block the epitope of some targets, preventing your primary antibody from binding (Fig.1). The...well as a cross-linker. This is because organic solvents can be quite harsh - lipids and soluble proteins...your target epitope (Fig. 1). So, while organic solvents may improve antibody binding for some targets,... can destroy it for others. That said, organic solvents’ impact on lipids means that they are able to ...

  13. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...ligated, resulting in the reciprocal crossover event. This event can have three general outcomes based on the...present; intramolecular events happen with greater frequency than intermolecular events, but any two sites ...can be used to induce site-specific recombination events. The system consists of two components derived ...recombinase catalyzes the site specific recombination event between two loxP sites, which can be located either... are on separate DNA molecules, a translocation event is generated at the loxP sites. How can I use Cre-lox... cassette) upstream of a gene of interest will prevent gene expression in the absence of Cre. In the presence...disrupted or deleted. Selection marker removal – in conventional mouse targeting, targeted clones are selected...

  14. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...output is generated through measurement of events. An event is something that passes through the machine...once you realize events are plotted on an invisible Cartesian plane (right.) Each event is plotted by the...it could be debris or unbound antibodies.) Each event read by the machine is plotted as a dot on an X,...axis to generate a dot plot (Figure 1). Clustered events are called a population.                           ...populations; this indicates the percentage of total events contained in the gated population. Heat maps are...on elevation maps, are used to show frequency of events, instead of relying on clustered dots. In a contour...contour plot, each line contains the same number of events. Thus, contour lines that are close together show...

  15. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    Published
    Nov. 17, 2015, 3:30 p.m.
    ...answer is probably no, but these fun and informative events are one of the main ways budding young scholars...societies. Freshers Fairs tend to be used as welcome events for new students that provide ways for students...opportunity for the societies to promote their upcoming events and intro sessions, and give out useful items and...research. The society also organises PhD information events and summer internship information sessions to help...organizes numerous recreational and educational events throughout the year, including a Christmas Chemistry...scientific disciplines, as well as social and networking events. Imperial Synthetic Biology Society, England The...for both students and the general public, career events, and networking meetups. The society hopes to help...

  16. Science Communication Snapshot: DayCon 2015

    Type
    Blog Post
    Published
    June 17, 2015, 9:04 p.m.
    ...News (SITN), we recently sponsored a first-time event called DayCon. DayCon is a one-day conference aimed...student volunteers worked hard to make this Saturday event a success, a true testament to the commitment of...HILS) made it possible to offer DayCon as a free event! Organizing a science conference for the public...DayCon Executive Board, I was fortunate to see the event planning unfold from the very beginning. The board...biomedical sciences and decided to offer a varied format event to keep the audience engaged. The day kicked off.... The future of DayCon DayCon and other similar events show how meaningful science outreach can be, both...scientific resources. SITN hopes to make DayCon a yearly event and to expand the conference to accommodate a larger...

  17. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    Published
    June 17, 2021, 1:17 p.m.
    ... for any wash…” 🎶- Barbara Di VenturaIf you follow Barbara Di Ventura on Twitter, you might have seen...graphical abstract, it’s a musical abstract,” says Di Ventura, a professor at the University of Freiburg. This...limit. pic.twitter.com/j6rhCCby2m — Barbara Di Ventura (@barbara_synbio) May 13, 2021 The original AraC...biotechnology, and almost every microbiology lab,” says Di Ventura. It relies on the sugar arabinose to activate transcription...reversibility that you cannot do with AraC,” says Di Ventura. BLADE activates transcription with the flick of... one of the several optogenetic tools that Di Ventura’s lab and Khammash's lab have created and shared...You can find all the plasmids deposited from Di Ventura's lab, including the popular light-inducible nuclear...

  18. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...cells, the single cell gate to exclude duplicate events, and the Live/Dead gate to focus your analysis ...my lymphocyte population (10.7% of all recorded events) based on their expected size. The strong signals...here. I am not worried about including irrelevant events in my analysis, as these will get excluded through...the next plot! Pro tip! If you only get very few events at the end of your gating pipeline, it might be..., you will likely need to exclude all duplicate events (two cells stuck together) from your single cells...while H and W do not; see Figure 2), single cell events will show up in a straight line one the plot, while...FSC-A we can gate on single cells and exclude any events where multiple cells were clumped together. (B)...

  19. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ...Testing AAV inventory for the entire collection! We are expanding our entire control AAV inventory to give...addition to our inventory of Cre-dependent GABA sensors. See all our GABA sensor AAV inventory here. pAAV.hSynap.iGABASnFR...service in 2016, we’ve added many new tools to our inventory of ready-to-use viral vectors. Here are some of...few months. You can also browse our entire AAV inventory. Our new AAVs include: EGFP-expressing AAV for...Encoded Calcium Sensors). We are expanding the inventory of these vectors to more serotypes. We currently... GCaMP7f and GCaMP7s. See all our biosensors inventory, and check out our latest vectors below: pGP-...growing field that we aim to support by growing our inventory of recombinases and recombinase-dependent vectors...

  20. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ...service in 2016, we’ve added many new tools to our inventory of ready-to-use viral vectors. Here are some of...few months. You can also browse our entire AAV inventory. Our new AAVs include: Control AAVs for serotype...always excited to add new control AAVs to our inventory since we know that scientists need these tools...popular control AAV encoding GFP. Check out our inventory of serotype testing AAV New control AAVs Plasmid...Dana et al., 2018). See our full biosensor AAV inventory New glutamate sensors Plasmid Serotype Name.../ Chemogenetics AAV We have also expanded our inventory of chemogenetic AAV for activating and inhibiting...be activated with FDA-approved drugs. See our inventory of DREADDs / Chemogenetics AAVs New DREADDs ...
Showing: 1 - 20 of 413 results